Abstract Lipid deposition inside the arterial wall is a key indicator of plaque vulnerability. An intravascular photoacoustic (IVPA) catheter considered promising device for quantifying amount lipid wall. Thus far, IVPA systems suffered from slow imaging speed (~50 s per frame) due to lack suitable laser source high-speed excitation molecular overtone vibrations. Here, we report an improvement in by two orders magnitude, 1.0 frame, enabled custom-built, 2-kHz master oscillator power...

Abstract Confronted with the severe situation that pace of resistance acquisition is faster than clinical introduction new antibiotics, health organizations are calling for effective approaches to combat methicillin‐resistant Staphylococcus aureus (MRSA) infections. Here, an approach treat MRSA through photolysis staphyloxanthin, antioxidant residing in microdomain S. membrane, reported. This photochemistry process uncovered transient absorption imaging and quantitated by spectroscopy, Raman...

Abstract Photonic technologies have made enormous impacts on modern medicine, advancing disease diagnostics and treatments as well health monitoring. A long‐standing challenge in the use of light its widespread effects photomedicine is finite penetration tissues. However, judiciously engineered optical fibers helped overcome this advance delivery to deep tissues with spatial precision desired accessibility. In recent years, development photonic including biomaterials, fiber...

Abstract A highly sensitive catheter probe is critical to catheter-based intravascular photoacoustic imaging. Here, we present a design on the basis of collinear alignment incident optical wave and photoacoustically generated sound within miniature housing for first time. Such with an outer diameter 1.6 mm provided efficient overlap between acoustic waves over imaging depth >6 in D 2 O medium. Intravascular lipid-laden atherosclerotic plaque perivascular fat was demonstrated, where...

Intravascular photoacoustic-ultrasound (IVPA-US) imaging is an emerging hybrid modality for the detection of lipid-laden plaques, as it provides simultaneous morphological and lipid-specific chemical information artery wall. Real-time display at video-rate speed are critical clinical utility IVPA-US technology. Here, we demonstrate a portable system capable up to 25 frames per second in real-time mode. This unprecedented was achieved by concurrent innovations excitation laser source, rotary...

Intravascular photoacoustic tomography is an emerging technology for mapping lipid deposition within arterial wall the investigation of vulnerability atherosclerotic plaques to rupture. By converting localized laser absorption in lipid-rich biological tissue into ultrasonic waves through thermoelastic expansion, intravascular uniquely capable imaging entire with chemical selectivity and depth resolution. However, technical challenges, including catheter sufficient sensitivity a functional...

Abstract Confronted with the rapid evolution and dissemination of antibiotic resistance, there is an urgent need to develop alternative treatment strategies for drug‐resistant pathogens. Here, unconventional approach presented restore susceptibility methicillin‐resistant S. aureus (MRSA) a broad spectrum conventional antibiotics via photo‐disassembly functional membrane microdomains. The microdomains based on effective photolysis staphyloxanthin, golden carotenoid pigment that gives its...

Label-free stimulated Raman scattering microscopy resolves amphotericin B orientation in fungal cell membrane.

Bacteria have evolved to cope with the detrimental effects of ROS using their essential molecular components. Catalase, a heme-containing tetramer protein expressed universally in most aerobic bacteria, plays an indispensable role scavenging excess hydrogen peroxide (H2O2). Here, through use wild-type and catalase-deficient mutants, we identified catalase as endogenous therapeutic target 400-420 nm blue light. Catalase residing inside bacteria could be effectively inactivated by light,...

Lipid deposition inside the arterial wall is a hallmark of plaque vulnerability. Based on overtone absorption C-H bonds, intravascular photoacoustic (IVPA) catheter promising technology for quantifying amount lipid and its spatial distribution wall. Thus far, clinical translation IVPA limited by slow imaging speed due to lack high-pulse-energy high-repetition-rate laser source lipid-specific first excitation at 1.7 μm. Here, we demonstrate potassium titanyl phosphate (KTP)-based optical...

Microbes have developed their own specific strategies to cope with reactive oxygen species (ROS). Catalase, a heme-containing tetramer expressed in broad range of aerobic fungi, shows remarkable efficiency degrading hydrogen peroxide (H

As the Internet becomes an increasingly vital source of medical information, quality and reliability brain tumor-related short videos on platforms such as TikTok Bilibili have not been adequately evaluated. Therefore, this study aims to assess these aspects explore factors influencing dissemination videos.

Recent advances in atherosclerotic plaque detection have shown that not only does lipid core size and depth play important roles rupture thrombi formation, but composition, especially cholesterol deposition, is equally determining lesion vulnerability. Here, we demonstrate a spectral analysis assisted photoacoustic imaging approach to differentiate map compositions within an artery wall. The based on the classification of curves obtained from sliding windows along time-of-flight signals via...

The chiral nematic phase structure, formed by the self-assembly of cellulose nanocrystals (CNCs) in an aqueous suspension and maintained a solid film, shows great potential for optical applications. To achieve complex structures devices, it is crucial to subject CNCs specific shearing processes, such as spinning printing. Understanding structural property changes CNC liquid crystal these processes utmost importance. In this study, we investigated effect adding tannic acid (TA) on rheological...

Abstract The prevalence of antibiotic resistance and tolerance in wound infection management poses a serious growing health threat, necessitating the exploration alternative approaches. Antimicrobial blue light therapy offers an appealing, non‐pharmacological solution. However, its practical application has been hindered by requirement for high irradiance levels (50–200 mW/cm 2 ), which particularly raises safety concerns. Here, light‐bathing strategy is introduced that employs prolonged,...

The rise of antibiotic resistant bacteria, e.g., methicillin-resistant Staphylococcus aureus (MRSA), has resulted in a widespread search for alternative treatments not reliant on traditional antibiotics. Silver nanoparticles (AgNPs) have long been known to exhibit antimicrobial activity against wide variety bacterial species. However, the clinical application AgNPs as an antibiotics limited by their toxicity at high concentrations. Here, via blue-light photolysis staphyloxanthin (STX),...

Abstract In the age of antimicrobial resistance, urgency by which novel therapeutic approaches need to be introduced into clinical pipeline has reached critical levels. Antimicrobial blue light (aBL), as an alternative approach, demonstrated promise a stand‐alone method, albeit with limited window activity. Work others indicates that treatment antibiotics increases production reactive oxygen species (ROS) may, in part, contribute bactericidal effects antibiotics. These findings suggest there...

Intravascular photoacoustic-ultrasound (IVPA-US) imaging and near-infrared spectroscopy-intravascular ultrasound (NIRS-IVUS) are two hybrid modalities that detect arterial lipid, with comparison necessary to understand the relative advantages of each. We performed in vivo ex IVPA-US iliac arteries Ossabaw swine metabolic syndrome (MetS) lean investigate sensitivity for early-stage atherosclerosis. repeated NIRS-IVUS histology. Both showed significantly greater lipid MetS vs. swine, but only...

Streptococcus agalactiae, also known as Group B (GBS), is increasingly recognized a major cause of soft tissue and invasive diseases in the elderly diabetic populations. Antibiotics like penicillin are used with great frequency to treat these infections, although antimicrobial resistance increasing among GBS strains underlines need for alternative methods not reliant on traditional antibiotics. granadaene pigment related hemolysin/cytolysin GBS, which critical pathogenesis diseases. Here, we...

“Coal measure gas,” excluding tight (low-permeability) sandstone gas, refers in this study to the self-sourcing unconventional natural gas stored coal, shale, and fine siltstone. It is characterized by various types of occurrences reservoirs, a distinct cyclic succession coal strata, widely distributed mosaic hydrocarbon accumulations, three configurations vertically multiple-stacked gas-bearing reservoirs. Coal from Shan 2 can be divided—according grain size sandstone,...

Abstract To realize online multiaxial fatigue damage assessment for the mechanical components in service, an cycle counting method is proposed coupled with segment processing technique and Wang‐Brow's relative equivalent strain concept. Meanwhile, considering all stress components, which contribute to on critical plane, a model without any weight coefficients also form of shear energy. Then, evaluation random loading developed by combining method. The experimental results showed that can be...