A5077594104- Photodynamic Therapy Research Studies
- Nanoplatforms for cancer theranostics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Antifungal resistance and susceptibility
- Bacterial Identification and Susceptibility Testing
- Bacterial biofilms and quorum sensing
- Bacteriophages and microbial interactions
- Ocular Infections and Treatments
- Genomics and Phylogenetic Studies
- Biosensors and Analytical Detection
- Essential Oils and Antimicrobial Activity
- Microfluidic and Capillary Electrophoresis Applications
- Antimicrobial Peptides and Activities
- Antimicrobial Resistance in Staphylococcus
- Gold and Silver Nanoparticles Synthesis and Applications
- Laser Applications in Dentistry and Medicine
- Cancer Research and Treatments
- Neonatal and Maternal Infections
- bioluminescence and chemiluminescence research
- Advanced Fluorescence Microscopy Techniques
- Streptococcal Infections and Treatments
- Medical and Biological Ozone Research
- Advanced Nanomaterials in Catalysis
- Protein Interaction Studies and Fluorescence Analysis
- Force Microscopy Techniques and Applications
Harvard University
2022-2024
Massachusetts General Hospital
2024
American Dental Association
2024
Novant Health Forsyth Medical Center
2024
Boston University
2017-2023
Photonics (United States)
2019-2022
Rapid antimicrobial susceptibility testing (AST) is urgently needed for treating infections with appropriate antibiotics and slowing down the emergence of antibiotic-resistant bacteria. Here, a phenotypic platform that rapidly produces AST results by femtosecond stimulated Raman scattering imaging deuterium oxide (D2 O) metabolism reported. Metabolic incorporation D2 O into biomass in single bacterium metabolic response to are probed as short 10 min after culture 70% medium, fastest among...
Abstract Confronted with the severe situation that pace of resistance acquisition is faster than clinical introduction new antibiotics, health organizations are calling for effective approaches to combat methicillin‐resistant Staphylococcus aureus (MRSA) infections. Here, an approach treat MRSA through photolysis staphyloxanthin, antioxidant residing in microdomain S. membrane, reported. This photochemistry process uncovered transient absorption imaging and quantitated by spectroscopy, Raman...
Significance The Candidate Phyla Radiation (CPR) is a large monophyletic lineage with poorly understood biology. Saccharibacteria are ultrasmall parasitic CPR bacteria highly reduced genomes that have made the transition from an environmental origin to mammals. We tested function and impact of arginine deiminase system (ADS), catabolism pathway likely acquired by mammal-associated during their environment-to-mammal niche transition. showed ADS not only helped facilitate Saccharibacterial...
Bacteriophages (phages) play critical roles in modulating microbial ecology. Within the human microbiome, factors influencing long-term coexistence of phages and bacteria remain poorly investigated. Saccharibacteria (formerly TM7) are ubiquitous members oral microbiome. These ultrasmall form episymbiotic relationships with their host impact physiology. Here, we showed that during surface-associated growth, a isolate (named TM7x) protects its bacterium, Schaalia odontolytica strain XH001)...
Abstract Confronted with the rapid evolution and dissemination of antibiotic resistance, there is an urgent need to develop alternative treatment strategies for drug‐resistant pathogens. Here, unconventional approach presented restore susceptibility methicillin‐resistant S. aureus (MRSA) a broad spectrum conventional antibiotics via photo‐disassembly functional membrane microdomains. The microdomains based on effective photolysis staphyloxanthin, golden carotenoid pigment that gives its...
Label-free stimulated Raman scattering microscopy resolves amphotericin B orientation in fungal cell membrane.
Bacteria have evolved to cope with the detrimental effects of ROS using their essential molecular components. Catalase, a heme-containing tetramer protein expressed universally in most aerobic bacteria, plays an indispensable role scavenging excess hydrogen peroxide (H2O2). Here, through use wild-type and catalase-deficient mutants, we identified catalase as endogenous therapeutic target 400-420 nm blue light. Catalase residing inside bacteria could be effectively inactivated by light,...
Abstract Host mucosal barriers possess an arsenal of defense molecules to maintain host-microbe homeostasis such as antimicrobial peptides and immunoglobulins. In addition these well-established molecules, we recently reported small RNAs (sRNAs)-mediated interactions between human oral keratinocytes Fusobacterium nucleatum (Fn), pathobiont with increasing implications in extra-oral diseases. Specifically, upon Fn infection, released Fn-targeting tRNA-derived sRNAs (tsRNAs), emerging class...
Abstract Saccharibacteria (formerly TM7) are a group of widespread and genetically diverse ultrasmall bacteria with highly reduced genomes that belong to Candidate Phyla Radiation, large monophyletic lineage poorly understood biology. Nanosynbacter lyticus type strain TM7x is the first member isolated from human oral microbiome. With restrained metabolic capacities, lives on surface of, forms an obligate episymbiotic relationship its bacterial host, Schaalia odontolytica XH001. The symbiosis...
Abstract High‐sensitivity chemical imaging offers a window to decipher the molecular orchestra inside living system. Based on vibrational fingerprint signatures, coherent Raman scattering microscopy provides label‐free approach map biomolecules and drug molecules cell. Yet, by near‐infrared (NIR) pulse excitation, sensitivity is limited millimolar concentration for endogenous biomolecules. Here, of stimulated (SRS) significantly boosted retinoid 34 micromolar via electronic preresonance in...
Microbes have developed their own specific strategies to cope with reactive oxygen species (ROS). Catalase, a heme-containing tetramer expressed in broad range of aerobic fungi, shows remarkable efficiency degrading hydrogen peroxide (H
Heme, a hydrophobic and cytotoxic macrocycle, is an essential cofactor in large number of proteins important for cell signaling. This must mean that heme mobilized from its place synthesis or entry into the to other parts where hemoproteins reside. However, cellular dynamics movement not well understood, part due inability image noninvasively live biological systems. Here, using high-resolution transient absorption microscopy, we showed storage distribution dynamic Caenorhabditis elegans....
Endodontic infections are known to be caused by pathogenic bacteria. Numerous previous studies found that both
As a stable and accurate biomarker, glycated hemoglobin (HbA1c) is clinically used to diagnose diabetes with threshold of 6.5% among total (Hb). Current methods such as boronate affinity chromatography involve complex processing large-volume blood samples. Moreover, these cannot measure HbA1c fraction at single-red cell (RBC) level, thus unable separate the contribution from other factors RBC lifetime. Here, we demonstrate spectroscopic transient absorption imaging approach that able...
Antimicrobial resistance is a significant concern to public health, and there pressing need develop novel antimicrobial therapeutic modalities.In this study, we investigated the capacity for quinine hydrochloride (Q-HCL) enhance effects of blue light ([aBL] 405 nm wavelength) against multidrug-resistant (MDR) Gram-negative bacteria in vitro vivo.Our findings demonstrated improvement inactivation MDR Pseudomonas aeruginosa Acinetobacter baumannii (planktonic cells biofilms) when aBL was...
Abstract The electrochromism of a water‐soluble naturally oxidized electrochromic polymer, ox‐PPE, is harnessed for rapid and facile bacterial detection, discrimination, susceptibility testing. ox‐PPE solution shows distinct colorimetric spectroscopic changes within 30 min when mixed with live bacteria. For the underlying mechanism, it found that responds to reducing species (e.g., cysteine glutathione) released by metabolically active This reduction reaction ubiquitous among various...
Abstract The prevalence of antibiotic resistance and tolerance in wound infection management poses a serious growing health threat, necessitating the exploration alternative approaches. Antimicrobial blue light therapy offers an appealing, non‐pharmacological solution. However, its practical application has been hindered by requirement for high irradiance levels (50–200 mW/cm 2 ), which particularly raises safety concerns. Here, light‐bathing strategy is introduced that employs prolonged,...
Saccharibacteria are episymbionts that require host-bacteria to grow. They positively associated with inflammatory diseases within the human microbiome, yet their mechanisms for interacting host and contributing remain unknown. This study investigated interactions between a Saccharibacterium (Nanosynbacter lyticus), its (Schaalia odontolytica), oral epithelial cells. The induced proinflammatory cytokines in cells, while were immune silent. Remarkably, dampened cytokine responses during...
The rise of antibiotic resistant bacteria, e.g., methicillin-resistant Staphylococcus aureus (MRSA), has resulted in a widespread search for alternative treatments not reliant on traditional antibiotics. Silver nanoparticles (AgNPs) have long been known to exhibit antimicrobial activity against wide variety bacterial species. However, the clinical application AgNPs as an antibiotics limited by their toxicity at high concentrations. Here, via blue-light photolysis staphyloxanthin (STX),...
Candida albicans (C. albicans), a major fungal pathogen, causes life-threatening infections in immunocompromised individuals. Fluconazole (FLC) is recommended as first-line therapy for treatment of invasive infections. However, the widespread use FLC has resulted increased antifungal resistance among different strains Candida, especially C. albicans, which leading source hospital-acquired Here, by hyperspectral stimulated Raman scattering imaging single cells fingerprint window and...
Streptococcus agalactiae, also known as Group B (GBS), is increasingly recognized a major cause of soft tissue and invasive diseases in the elderly diabetic populations. Antibiotics like penicillin are used with great frequency to treat these infections, although antimicrobial resistance increasing among GBS strains underlines need for alternative methods not reliant on traditional antibiotics. granadaene pigment related hemolysin/cytolysin GBS, which critical pathogenesis diseases. Here, we...
Abstract The prevalence of antibiotic resistance and tolerance in wound infection management poses a serious growing health threat, necessitating the exploration alternative approaches. Antimicrobial blue light therapy offers an appealing, non-pharmacological solution. However, its practical application has been hindered by requirement for high irradiance levels, which particularly raises safety concerns. Here, we introduce light-bathing strategy that employs prolonged, continuous exposure...
Abstract We developed a translational prototype antimicrobial blue light (ABL) device for treating skin wounds with ABL. Partial-thickness surgical were created in live swine (an animal whose is considered the most like human skin), then heavily contaminated and left untreated 24 hours methicillin-resistant Staphylococcus aureus (MRSA). ABL treatment stabilized reduced MRSA infection by greater than 4 orders of magnitude (>99.99%; P < .0001) compared same animal, after only 2...
Hemozoin, the heme detoxification end product in malaria parasites during their growth red blood cells (RBCs), serves as an important marker for diagnosis and treatment target of disease. However, current method hemozoin-targeted drug screening mainly relies on vitro β-hematin inhibition assays, which may lead to false-positive events due under-representation real hemozoin crystal. Quantitative situ imaging is highly desired high-throughput antimalarial drugs elucidating mechanisms drugs. We...