A5021743666- Spectroscopy Techniques in Biomedical and Chemical Research
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Spectroscopy and Chemometric Analyses
- Cell Image Analysis Techniques
- Photoreceptor and optogenetics research
- Metabolomics and Mass Spectrometry Studies
- Thermography and Photoacoustic Techniques
- Cancer, Stress, Anesthesia, and Immune Response
- Nanoplatforms for cancer theranostics
- Gold and Silver Nanoparticles Synthesis and Applications
- Cancer, Lipids, and Metabolism
- Advanced Biosensing Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Optical Coherence Tomography Applications
- 3D Printing in Biomedical Research
- Malaria Research and Control
- AI in cancer detection
- Brain Tumor Detection and Classification
- Microfluidic and Capillary Electrophoresis Applications
- Protein Interaction Studies and Fluorescence Analysis
- Plasmonic and Surface Plasmon Research
- Digital Imaging for Blood Diseases
- Immune cells in cancer
- Luminescence and Fluorescent Materials
Cygnal Therapeutics (United States)
2021-2022
Boston University
2018-2022
Purdue University West Lafayette
2016-2017
Weldon City Schools
2016
National Taiwan University
2014-2015
Osaka University
2013
Abstract Solid tumours are innervated by nerve fibres that arise from the autonomic and sensory peripheral nervous systems 1–5 . Whether neo-innervation of pain-initiating neurons affects cancer immunosurveillance remains unclear. Here we show melanoma cells interact with nociceptor neurons, leading to increases in their neurite outgrowth, responsiveness noxious ligands neuropeptide release. Calcitonin gene-related peptide (CGRP)—one such nociceptor-produced neuropeptide—directly exhaustion...
Abstract Increased glycolysis is considered as a hallmark of cancer. Yet, cancer cell metabolic reprograming during therapeutic resistance development under-studied. Here, through high-throughput stimulated Raman scattering imaging and single analysis, we find that cisplatin-resistant cells exhibit increased fatty acids (FA) uptake, accompanied by decreased glucose uptake lipogenesis, indicating reprogramming from to FA dependent anabolic energy metabolism. A index incorporating derived...
Flow cytometry is one of the most important technologies for high-throughput single-cell analysis. Fluorescent labeling acts as primary approach cellular analysis in flow cytometry. Nevertheless, fluorescent tags are not applicable to all cases, especially small molecules, which may significantly perturb biological functionality. Spontaneous Raman scattering offers capability non-invasively detect chemical contents cells but suffers from slow data acquisition. In order achieve label-free...
In situ measurement of cellular metabolites is still a challenge in biology. Conventional methods, such as mass spectrometry or fluorescence microscopy, would either destroy the sample introduce strong perturbations to target molecules. Here, we present multiplex stimulated Raman scattering (SRS) imaging cytometry label-free single-cell analysis platform with chemical specificity and high-throughput capabilities. Using SRS cytometry, studied metabolic responses human pancreatic cancer cells...
Stimulated Raman scattering (SRS) microscopy is an emerging platform for vibrational imaging of living systems. We present microsecond-scale SRS spectroscopic by temporally tuning two spectrally focused pulses through a resonant delay line. Our able to acquire spectrum in 42 μs and form spectral image 40,000 pixels within 3.3 s. Spectroscopic identification single bacteria fungi blood 4-D (x–y–z–λ) intracellular organelles live C. elegans are demonstrated.
Abstract Volumetric imaging allows global understanding of three-dimensional (3D) complex systems. Light-sheet fluorescence microscopy and optical projection tomography have been reported to image 3D volumes with high resolutions at speeds. Such methods, however, usually rely on fluorescent labels for chemical targeting, which could perturb the biological functionality in living We demonstrate Bessel-beam-based stimulated Raman (SRP) label-free volumetric imaging. Our SRP microscope enables...
Abstract Development of molecular probes holds great promise for early diagnosis aggressive prostate cancer. Here, 2‐[3‐(1,3‐dicarboxypropyl) ureido] pentanedioic acid (DUPA)‐conjugated ligand and bis‐isoindigo‐based polymer (BTII) are synthesized to formulate semiconducting nanoparticles (BTII‐DUPA SPN) as a prostate‐specific membrane antigen (PSMA)‐targeted probe cancer imaging in the NIR‐II window. Insights into interaction with biological targets from single cell whole organ obtained by...
Abstract High‐sensitivity chemical imaging offers a window to decipher the molecular orchestra inside living system. Based on vibrational fingerprint signatures, coherent Raman scattering microscopy provides label‐free approach map biomolecules and drug molecules cell. Yet, by near‐infrared (NIR) pulse excitation, sensitivity is limited millimolar concentration for endogenous biomolecules. Here, of stimulated (SRS) significantly boosted retinoid 34 micromolar via electronic preresonance in...
Graphene grain boundaries (GBs) and other nanodefects can deteriorate electronic properties. Here, using transient absorption (TA) microscopy we directly visualized GBs by TA intensity increase due to change in density of state. We also observed a faster decay defect-accelerated carrier relaxation the GB area. By line-illumination parallel detection, increased imaging speed 1000 frames per second, which is 6 orders magnitude than Raman microscopy. Combined with resonant optical delay tuner...
As a stable and accurate biomarker, glycated hemoglobin (HbA1c) is clinically used to diagnose diabetes with threshold of 6.5% among total (Hb). Current methods such as boronate affinity chromatography involve complex processing large-volume blood samples. Moreover, these cannot measure HbA1c fraction at single-red cell (RBC) level, thus unable separate the contribution from other factors RBC lifetime. Here, we demonstrate spectroscopic transient absorption imaging approach that able...
Abstract Microscopy based on non-fluorescent absorption dye staining is widely used in various fields of biomedicine for 400 years. Unlike its fluorescent counterpart, microscopy lacks proper methodologies to realize vivo applications with a sub-femtoliter 3D resolution. Regardless the most advanced high-resolution photoacoustic microscopy, spatial resolution still unattainable and imaging speed relatively slow. In this paper, two-photon mechanism, we demonstrated label free laser-scanning...
Voltage imaging allows mapping of the membrane potential in living cells. Yet, current intensity-based approaches are limited to relative changes, missing important information conveyed by absolute value voltage. This challenge arises from various factors affecting signal intensity, such as concentration, illumination and photobleaching. Here, we demonstrate electronic preresonance hyperspectral stimulated Raman scattering (EPR-hSRS) for spectroscopic detection voltage using a...
Hemozoin, the heme detoxification end product in malaria parasites during their growth red blood cells (RBCs), serves as an important marker for diagnosis and treatment target of disease. However, current method hemozoin-targeted drug screening mainly relies on vitro β-hematin inhibition assays, which may lead to false-positive events due under-representation real hemozoin crystal. Quantitative situ imaging is highly desired high-throughput antimalarial drugs elucidating mechanisms drugs. We...
Crystal engineering is a practical approach for tailoring material properties. This has been widely studied modulating optical and electrical properties of semiconductors. However, the organic molecular crystals are difficult to control following similar route. In this Letter, we demonstrate that engineered Alq3 Ir(ppy)3 complexes, which commonly used in light-emitting technologies, possess intriguing functional Specifically, these structures not only process efficient low-energy induced...
We demonstrate an ambient light coherent anti-Stokes Raman scattering microscope that allows CARS imaging to be operated under environmental for field use. The signal is modulated at megahertz frequency and detected by a photodiode equipped with lab-built resonant amplifier, then extracted through lock-in amplifier. filters in both the spectral domain effectively blocked room contamination of image. In situ hyperspectral tumor tissue demonstrated.
Tumor innervation has recently been documented and characterized in various settings tumor types. However, the role that nerves innervating tumors play pathogenesis of cancer not clarified. In this study, we searched for neural signaling from bulk RNA sequencing The Cancer Genome Atlas (TCGA) dataset looked patterns interactions between different cell types within environment. Using a presynapse signature (PSS) as probe, showed multiple stromal crosstalk and/or contribute to signals. Based...
We report microsecond-scale acquisition of simulated Raman spectra by resonant delay-line tuning. Our scheme improved the spectral speed 100 times compared to previous works motorized translational-stage 4-D images (x-y-z-λ) from highly dynamic organelles in live C. elegans was demonstrated.
Voltage imaging has become an emerging technique to record membrane potential change in living cells. Yet, compared the conventional electrophysiology, approaches are still limited relative changes, losing important information conveyed by absolute value of voltage. This challenge comes from several factors affecting signal intensity, such as concentration, illumination and photobleaching. Spectroscopy is a quantitative method that shows report state molecules situ. Here, we apply electronic...
Development of specific molecular probes holds great promise for early diagnosis aggressive prostate cancer (PCa). Here, 2-[3-(1,3-dicarboxypropyl) ureido] pentanedioic acid (DUPA)-conjugated ligand and bis-isoindigo-based polymer (BTII) are synthesized to formulate semiconducting nanoparticles (BTII-DUPA SPN) as a prostate-specific membrane antigen (PSMA)-targeted probe PCa imaging in the NIR-II window. Insights into interaction with biological targets from single-cell whole-organ levels...
Trade-off between resolution and penetration restricts performances of microscopes. We realize a hybrid modality multiphoton fluorescence photoacoustic microscopy via an optical modulation technique. The new features both high-resolution deep-penetration.
ABSTRACT Increased aerobic glycolysis is widely considered as a hallmark of cancer. Yet, cancer cell metabolic reprograming during development therapeutic resistance under-studied. Here, through high-throughput stimulated Raman scattering imaging and single analysis, we found that cisplatin-resistant cells exhibit increased uptake exogenous fatty acids, accompanied with decreased glucose de novo lipogenesis, indicating reprogramming from dependent to acid beta-oxidation anabolic energy...
Stimulated Raman scattering (SRS) microscopy is a promising technique for label-free imaging of living systems. We demonstrate microsecond-scale SRS spectral by tuning two spectrally focused pulses temporally through resonant delay-line. Our platform acquired an spectrum within 42 microseconds and formed image composed 40,000 pixels in real-time.
A hyperspectral image corresponds to a data cube with two spatial dimensions and one spectral dimension. Through linear un-mixing, images can be decomposed into signatures of pure components as well their concentration maps. Due this distinct advantage on component identification, imaging becomes rapidly emerging platform for engineering better medicine expediting scientific discovery. Among various techniques, stimulated Raman scattering (HSRS) microscopy acquires in pixel-by-pixel scanning...
We present a stimulated Raman scattering imaging system which acquires spectrum within 20 µs. A U-Net deep learning network is applied to maintain the sensitivity at high speeds, enabling high-throughput label-free spectroscopic of cells and tissues. © 2019 The Author(s)