A5002332222- Single-cell and spatial transcriptomics
- Cell Image Analysis Techniques
- Genomics and Phylogenetic Studies
- Diabetes and associated disorders
- Protist diversity and phylogeny
- Extracellular vesicles in disease
- Cancer Genomics and Diagnostics
- Mitochondrial Function and Pathology
- Gene expression and cancer classification
University of Toronto
2021-2024
Abstract Single-cell RNA sequencing (scRNA-seq) has revolutionized our understanding of cell types and tissues. However, empty droplets poor quality cells are often captured in single genomics experiments need to be removed avoid type interpretation errors. Many automated manual methods exist identify or droplets, such as minimum count thresholds comparing the gene expression profile an individual overall background experiment. A versatile approach is use unbalanced splice ratios droplets....
Abstract Background Model organisms allowing for longitudinal examinations of liver disease pathogenesis are pivotal the development new therapeutic modalities. The eastern North American woodchuck develops chronic hepatitis and cancer after virus (WHV) infection, mirroring aspects natural history human B (HBV). However, cellular landscape cell-level relevance WHV infection to HBV is currently uncharacterized. Methods We employed single-cell RNA sequencing (RNA-seq) generate an atlas healthy...
Single-cell transcriptomics can profile thousands of cells in a single experiment and identify novel cell types, states dynamics wide variety tissues organisms. Standard experimental protocols analysis workflows have been developed to create single-cell transcriptomic maps from tissues. This tutorial focuses on how interpret these data other biologically relevant patterns with the objective creating an annotated map cells. We recommend three-step workflow including automatic annotation...
The naked mole-rat (NMR; Heterocephalus glaber ) is a eusocial subterranean rodent with highly unusual set of physiological traits that has attracted great interest amongst the scientific community. However, genetic basis most these not been elucidated. To facilitate our understanding molecular mechanisms underlying NMR physiology and behaviour, we generated long-read chromosomal-level genome assembly NMR. This was subsequently annotated incorporated into multiple whole alignments in Ensembl...