A5088892989- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Cellular Mechanics and Interactions
- Cancer-related gene regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetics and Neurodevelopmental Disorders
- Reproductive System and Pregnancy
- RNA Research and Splicing
- Microtubule and mitosis dynamics
- Silk-based biomaterials and applications
- RNA modifications and cancer
- Protist diversity and phylogeny
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Chromosomal and Genetic Variations
- DNA Repair Mechanisms
- Single-cell and spatial transcriptomics
- Skin and Cellular Biology Research
- RNA Interference and Gene Delivery
- Biocrusts and Microbial Ecology
- Epigenetics and DNA Methylation
- Genomics and Phylogenetic Studies
University of California, San Francisco
2023-2024
Broad Center
2023
Weizmann Institute of Science
2017-2022
Austrian Academy of Sciences
2014-2016
Institute of Molecular Biotechnology
2014
The N6-methyladenosine (m 6 A) modification is the most prevalent post-transcriptional mRNA modification, regulating decay and splicing. It plays a major role during normal development, differentiation, disease progression. regulated by set of writer, eraser, reader proteins. YTH domain family proteins consists three homologous m A-binding proteins, Ythdf1, Ythdf2, Ythdf3, which were suggested to have different cellular functions. However, their sequence similarity tendency bind same targets...
Isolating human MEK/ERK signaling-independent pluripotent stem cells (PSCs) with naive pluripotency characteristics while maintaining differentiation competence and (epi)genetic integrity remains challenging. Here, we engineer reporter systems that allow the screening for defined conditions induce molecular functional features of pluripotency. Synergistic inhibition WNT/β-CATENIN, protein kinase C (PKC), SRC signaling consolidates induction teratoma-competent PSCs, capacity to differentiate...
The recent derivation of human trophoblast stem cells (TSCs) from placental cytotrophoblasts and blastocysts opened opportunities for studying the development function placenta. Recent reports have suggested that naïve, but not primed, pluripotent (PSCs) retain an exclusive potential to generate TSCs. Here we report that, in absence WNT stimulation, transforming growth factor β (TGF-β) pathway inhibition leads direct robust conversion primed PSCs into resulting PSC-derived TSC lines exhibit...
iPSCs derived from fibroblasts with mutant type VII collagen were genetically corrected and used as cell therapy in mice recessive dystrophic epidermolysis bullosa.
The ability to reprogram somatic cells into induced pluripotent stem (iPSCs) with four transcription factors Oct4, Sox2, Klf4 and cMyc (abbreviated as OSKM) 1 has provoked interest define the molecular characteristics of this process 2-7 . Despite important progress, dynamics epigenetic reprogramming at high resolution in correctly reprogrammed iPSCs throughout entire remain largely undefined. This gap understanding results from inefficiency conventional methods coupled difficulty...
Abstract Different conditions have been devised to isolate MEK/ERK signalling independent human naïve pluripotent stem cells (PSCs) that are distinct from conventional primed PSCs and better correspond pre-implantation developmental stages. While the described thus far endow with different extents of naivety features, isolating retain characteristics ground state pluripotency while maintaining differentiation potential genetic integrity, remains a major challenge. Here we engineer reporter...
The naked mole-rat (NMR; Heterocephalus glaber ) is a eusocial subterranean rodent with highly unusual set of physiological traits that has attracted great interest amongst the scientific community. However, genetic basis most these not been elucidated. To facilitate our understanding molecular mechanisms underlying NMR physiology and behaviour, we generated long-read chromosomal-level genome assembly NMR. This was subsequently annotated incorporated into multiple whole alignments in Ensembl...
Abstract The Nucleosome Remodeling and Deacytelase (NuRD) complex is a co-repressive involved in many pathological physiological processes the cell. Previous studies have identified one of its components, Mbd3, as potent inhibitor for reprogramming somatic cells to pluripotency. Following OSKM induction, early partial depletion Mbd3 protein followed by applying naïve ground-state pluripotency conditions, results highly efficient near-deterministic generation mouse iPS cells. Increasing...
Summary Cells of the trophoblast lineage constitute major part placental tissues in higher mammals. Recent derivation human stem cells (TSC) from cytotrophoblasts (CT) and naïve PSCs opens new opportunities for studying development function placenta. Several recent reports have suggested that retain an exclusive potential to give rise bona fide TSCs. Here we report inhibition TGFβ pathway avoiding WNT stimulation, leads direct robust conversion primed pluripotent into Systematic side by...
The epigenetic dynamics of iPSC reprogramming in correctly reprogrammed cells at high resolution and throughout the entire process, remain largely undefined. This gap understanding results from inefficiency conventional methods coupled with difficulty prospectively isolating rare that eventually reprogram into iPSCs. Here we characterize cell fate conversion fibroblast to using radically efficient murine systems. comprehensive characterization provides single day dynamic changes levels gene...
The Nucleosome Remodeling and Deacytelase (NuRD) complex is a corepressive involved in many pathological physiological processes the cell. Previous studies have identified one of its components, Mbd3, as potent inhibitor for reprogramming somatic cells to pluripotency. Following OSKM induction, early partial depletion Mbd3 protein followed by applying naïve ground-state pluripotency conditions, results highly efficient near-deterministic generation mouse iPS cells. Increasing evidence...