A5084744964- Reproductive Biology and Fertility
- Prenatal Screening and Diagnostics
- Genomics and Chromatin Dynamics
- Reproductive Health and Technologies
- Ovarian function and disorders
- RNA Research and Splicing
- Assisted Reproductive Technology and Twin Pregnancy
- Renal and related cancers
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Reproductive System and Pregnancy
- Genetics and Neurodevelopmental Disorders
- LGBTQ Health, Identity, and Policy
- Cancer-related Molecular Pathways
- 3D Printing in Biomedical Research
- Genomic variations and chromosomal abnormalities
- Heat shock proteins research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- DNA Repair Mechanisms
- Gynecological conditions and treatments
- Ovarian cancer diagnosis and treatment
- MicroRNA in disease regulation
- Single-cell and spatial transcriptomics
- Pregnancy and preeclampsia studies
Tel Aviv Sourasky Medical Center
2014-2024
Tel Aviv University
2001-2024
Weizmann Institute of Science
2001-2019
Kaplan Medical Center
2003
Isolating human MEK/ERK signaling-independent pluripotent stem cells (PSCs) with naive pluripotency characteristics while maintaining differentiation competence and (epi)genetic integrity remains challenging. Here, we engineer reporter systems that allow the screening for defined conditions induce molecular functional features of pluripotency. Synergistic inhibition WNT/β-CATENIN, protein kinase C (PKC), SRC signaling consolidates induction teratoma-competent PSCs, capacity to differentiate...
Binding of transcription factors (TFs) to regulatory sequences is a pivotal step in the control gene expression. Despite many advances characterization sequence motifs recognized by TFs, our ability quantitatively predict TF binding different still limited. Here, we present novel experimental assay termed BunDLE-seq that provides quantitative measurements thousands fully designed 200 bp length within single experiment. Applying this two yeast demonstrate outside core site profoundly affect...
Genetically identical cells exhibit large variability (noise) in gene expression, with important consequences for cellular function. Although the amount of noise decreases and is thus partly determined by mean expression level, extent to which different promoter sequences can deviate away from this trend not fully known. Here, we present a high-throughput method measuring promoter-driven thousands designed synthetic promoters parallel. We use it investigate how encode levels find that...
Despite much research, our understanding of the architecture and cis-regulatory elements human promoters is still lacking. Here, we devised a high-throughput assay to quantify activity approximately 15,000 fully designed sequences that integrated expressed from fixed location within genome. We used this method investigate thousands native preinitiation complex (PIC) binding regions followed by in-depth characterization sequence motifs underlying promoter activity, including core TF sites....
LAP2beta is an integral membrane protein of the nuclear envelope involved in chromatin and architecture. Using yeast two-hybrid system, we have cloned a novel LAP2beta-binding protein, mGCL, which contains BTB/POZ domain mouse homologue Drosophila germ-cell-less (GCL) protein. In embryos, GCL was shown to be essential for germ cell formation localized envelope. Here, show that, mammalian cells, co-localized with Nuclear fractionation studies reveal that mGCL acts as matrix component not...
Abstract Human preimplantation development involves extensive remodeling of RNA expression and splicing. However, its transcriptome has been compiled using short-read sequencing data, which fails to capture most full-length mRNAs. Here, we generate an isoform-resolved early human by performing long- on 73 embryos spanning the zygote blastocyst stages. We identify 110,212 unannotated isoforms transcribed from known genes, including highly conserved protein-coding loci key developmental...
Abstract STUDY QUESTION Can the BlastAssist deep learning pipeline perform comparably to or outperform human experts and embryologists at measuring interpretable, clinically relevant features of embryos in IVF? SUMMARY ANSWER The can measure a comprehensive set interpretable either these features, WHAT IS KNOWN ALREADY Some studies have applied developed ‘black-box’ algorithms predict embryo viability directly from microscope images videos but lack interpretability generalizability. Other...
The coordinated function of the different compartments follicle, oocyte and somatic cumulus/granulosa cells, is enabled by presence a network cell-to-cell communication generated gap junctions. Connexin 43 (Cx43) most abundant junction protein expressed ovarian follicle. expression Cx43 subjected to control gonadotropins as follows: FSH up-regulates, whereas LH down-regulates its levels. aim this study was explore mechanism which reduces levels identify signal transduction pathway involved...
Purpose: To compare the morphokinetic parameters of pre-implantation development between embryos women advanced maternal age (AMA) and young women. Methods: Time-lapse microscopy was used to variables 495 AMA ≥ 42 years 653 patients (<age 38 years) who underwent IVF in our unit. Developmental events annotated analyzed include observed cell divisions correlation timing fertilization, synchrony second (s2) third cycles (s3) duration (cc2) cleavages (cc3). Results: No significant differences...
Advances in molecular cell biology, medical research, and drug development are driving a growing need for technologies that enable imaging the dynamics of physiological processes simultaneously numerous non-adherent living cells. Here we describe platform technology software – CKChip system enables continuous, fluorescence-based thousands individual cells, each held at given position ("address") on chip. The allows sequential monitoring, manipulation kinetic analyses effects drugs,...
Quantitative and qualitative spermatogenic impairments are major causes of men's infertility. Although in vitro fertilization (IVF) is effective, some couples persistently fail to conceive. To identify causal variants patients with severe male infertility factor repeated IVF failures, we sequenced the exome two consanguineous family members who underwent several failed cycles were diagnosed low sperm count motility. We identified a rare homozygous nonsense mutation previously uncharacterized...
Most cases of colorectal cancer (CRC) are initiated by inactivation mutations in the APC gene, which is a negative regulator Wnt-β-catenin pathway. Patients with familial adenomatous polyposis (FAP) inherit germline mutation one allele, and loss second allele leads to development polyps that will turn malignant if not removed. It fully understood molecular mechanisms activated when occurs.Two FAP human embryonic stem cell (hESCs) lines were derived from mutated embryos following...
What is the optimal timing for blastomere biopsy during 8-cell stage, at which embryos will have best implantation potential?Fast-cleaving that are biopsied last quarter (Q4) of stage and less affected by procedure, their potential better than earlier (Q1-Q3).Blastomer from cleavage-stage usually performed on morning Day 3 when 6- to still preferred method preimplantation genetic diagnosis (PGD) monogentic disorders or chromosomal translocations. Human remain a relatively long 'arrest phase'...