A5102951521- RNA Research and Splicing
- Nuclear Structure and Function
- Genomics and Chromatin Dynamics
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Metabolism, Diabetes, and Cancer
- Cancer-related Molecular Pathways
- Acute Ischemic Stroke Management
- FOXO transcription factor regulation
- Pancreatic function and diabetes
- Diabetes Management and Research
- Data-Driven Disease Surveillance
- Pituitary Gland Disorders and Treatments
- Adrenal Hormones and Disorders
- Diabetes Treatment and Management
- Caveolin-1 and cellular processes
- Ophthalmology and Eye Disorders
- Nutritional Studies and Diet
- Data Quality and Management
- Estrogen and related hormone effects
- Mesenchymal stem cell research
- Wound Healing and Treatments
- Diet and metabolism studies
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Peroxisome Proliferator-Activated Receptors
- Renin-Angiotensin System Studies
Tel Aviv Sourasky Medical Center
2014-2025
Tel Aviv University
2001-2025
Herzliya Medical Center
2021
Sheba Medical Center
2001-2014
Rabin Medical Center
2005
Weizmann Institute of Science
2005
Nuclear-envelope proteins have been implicated in diverse and fundamental cell functions, among them transcriptional regulation. Gene expression at the territory of nuclear periphery is known to be repressed by epigenetic modifications such as histone deacetylation methylation. However, mechanism which nuclear-envelope are involved still obscure. We previously shown that LAP2β, an integral protein contains chromatin-binding LEM domain, was able repress activity E2F5-DP3 heterodimer. Here, we...
LAP2beta is an integral membrane protein of the nuclear envelope involved in chromatin and architecture. Using yeast two-hybrid system, we have cloned a novel LAP2beta-binding protein, mGCL, which contains BTB/POZ domain mouse homologue Drosophila germ-cell-less (GCL) protein. In embryos, GCL was shown to be essential for germ cell formation localized envelope. Here, show that, mammalian cells, co-localized with Nuclear fractionation studies reveal that mGCL acts as matrix component not...
Context: Some clinical resemblance may exist between obesity, particularly abdominal and Cushing’s syndrome. This has stimulated ongoing interest in the role of cortisol's secretion pattern, control metabolism obesity. Goals: To investigate whether basal levels cortisol differ healthy people with obesity individuals normal weight Design: Total, free, salivary were tested at baseline state after 1 g ACTH stimulation 60 subjects 54 lean controls. Results: Baseline total was lower compared to...
Abstract This study was designed to improve blood glucose level predictability and future hypoglycemic hyperglycemic event alerts through a novel patient‐specific supervised‐machine‐learning (SML) analysis of based on continuous‐glucose‐monitoring system (CGM) that needs no human intervention, minimises false‐positive alerts. The CGM data over 7 50 non‐consecutive days from 11 type‐1 diabetic patients aged 18 39 with mean HbA1C 7.5% ± 1.2% were analysed using four SML models. algorithm...
Polyploidy has been recognized for many years as an important hallmark of cancer cells. Polyploid cells can arise through cell fusion, endoreplication and abortive cycle. The inner nuclear membrane protein LAP2β plays key roles in envelope breakdown reassembly during mitosis, initiation replication transcriptional repression. Here we studied the function maintenance ploidy state, a role which not yet assigned to this protein.By knocking down expression LAP2β, using both viral non-viral RNAi...
Background Cerebrovascular disease is a leading cause of mortality and disability. Common risk assessment tools for stroke are based on the Framingham equation, which relies traditional cardiovascular factors to predict an acute event in near decade. However, no currently available near/impending stroke, might alert patients at seek immediate preventive action (eg, anticoagulants atrial fibrillation, control hypertension). Objective Here, we propose that algorithm internet search queries can...
Abstract Estradiol-17β (E2) and the Foxo1 transcription factor have each been implicated in regulation of β-cell proliferation. Interaction between Foxo1and estrogen receptor alpha (ERα), effecting cell cycle, has demonstrated breast cancer cells, but not studied thus far β-cells. Using human islets INS1-E line, this study investigated contribution to E2-mediated replication. expression was knocked down cells using siRNA activity inhibited with a specific inhibitor (AS1842856). Cells were...
Inhibition of endothelial nitric oxide synthase (eNOS) accelerates atherosclerosis in ApoE-null mice by impairing the balance between angiotensin II (AII) and NO. Our previous data suggested a role for PPAR α deleterious effect renin-angiotensin system (RAS). We tested hypothesis that lacking (DKO mice) would be resistant to proatherogenic NOS inhibition. DKO fed Western diet were immune 23% worsening aortic sinus plaque area seen animals under 12 weeks inhibition with subpressor dose...
We assessed the accuracy of FreeStyle Mini (FSM) meter for use in glycemic clamp and meal protocols comparison with HemoCue Glucose 201 DM Analyzer (HemoCue) YSI 2300 STAT Oxidase (YSI).Seven volunteers type 2 diabetes mellitus, 35-69 years old, underwent a frequently sampled test graded hyperglycemic test, on two separate days, one undergoing each twice. Samples glucose measurements were obtained from arterialized venous blood. A total 420 samples (with levels ranging 63 to 388 mg/dl)...
Abstract Disclosure: Y. Sofer: None. E. Osher: Greenman: K.M. Tordjman: G. Shenkerman: M. Yaron: Marcus-Perlman: Moshe: S. Shaklai: R. Limor: abiri: D. Cantrell: N. Stern: Objective: Some clinical resemblance may exist between obesity, particularly abdominal and Cushing’s syndrome. This has stimulated ongoing interest in the role of cortisol's secretion pattern, control metabolism obesity. Goals: To compare basal dynamic cortisol response to an intravenous bolus dose 1 ug ACTH healthy obese...
Background: Estradiol 17-beta (E2) enhances the function and survival of pancreatic beta-cells but its clinical use has been questioned due to concerns regarding oncogenic potential feminizing effects in males. The G-protein coupled estrogen receptor (GPER), expressed islets, exhibits estrogenic beta-cell protective effects, without nuclear ERs. Here, we examine outcome selective activation three receptors ERα, ERβ GPER on replication, human islets INS1-E rodent β-cell line, under...
Abstract Introduction Factors influencing development and course of ophthalmopathy, the most common extrathyroidal manifestation Graves’ disease, remain poorly defined. Clinical Case A 46 year old healthy, non-smoking, medical secretary, presented with disease. Laboratory investigation showed thyrotoxicosis high thyroid stimulation immunoglobulin (TSI) levels (1608 %, normal <140%). At presentation there was no evidence eye disease on clinical exam orbital MRI. Treatment Methimazol...
Abstract Disclosure: S. Shaklai: None. Y. Greenman: E. Yom-Tov: Background: Hot flashes, a hallmark of the menopausal transition, frequently coincides with CNS manifestations disturbed sleep, cognition, and mood. How these relate is an active area research. Objective: To evaluate association hot flashes specific manifestations, as expressed by women on one largest social media platforms (Reddit). Methods: We downloaded 4772 posts from Reddit’s Menopause group. 586 randomly sampled were...
Estrogen exerts its effects through genomic and rapid extra-nuclear signaling pathways. The classic estrogen receptors, ERα ERβ have demonstrated favorable on beta-cell function survival but their clinical utility has been questioned owing to oncogenic potential concerns regarding feminizing in men. Recent work indicates, however, that the G-protein coupled receptor (GPER), expressed pancreatic islet cells retains estrogenic protective effects, without shortcomings of ERs. GPER activation...
Background: With the growing prevalence of obesity, there is also a rise in incidence metabolic syndrome (MS). It characterizes by hyperinsulinemia, increased fasting glucose, hypertriglyceridemia, low HDL, waist circumference and hypertension.Insulin-degrading enzyme (IDE) major responsible for insulin degradation among other proteins linked to glucose metabolism such as glucagon. Using genome wide associated studies, IDE was identified diabetes susceptibility gene. Furthermore, inhibition...
Abstract Cerebrovascular disease is a leading cause of mortality and disability an immense global burden. It partly related to aging in metabolic syndrome-promoting environment. Prevention strategies are insufficient: they rely on intermittent screening predominantly high-risk individuals, while most cases occur the intermediate risk population. Screening algorithms like Framingham Risk Score predict events next decade using traditional factors (age, diabetes, hypertension, obesity,...