A5007513441- Circular RNAs in diseases
- RNA modifications and cancer
- RNA Research and Splicing
- Cancer-related gene regulation
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Plant Molecular Biology Research
- Light effects on plants
- Cancer Risks and Factors
- Vibrio bacteria research studies
- Pregnancy and preeclampsia studies
- Aquaculture disease management and microbiota
- Mitochondrial Function and Pathology
- Acute Myeloid Leukemia Research
- Blood donation and transfusion practices
- Immune Cell Function and Interaction
- Circadian rhythm and melatonin
- Ovarian cancer diagnosis and treatment
- Gastrointestinal disorders and treatments
- Invertebrate Immune Response Mechanisms
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- Reproductive System and Pregnancy
- Hernia repair and management
- Pancreatic function and diabetes
Stanford University
2019-2024
Neurosciences Institute
2022-2024
Institute for Stem Cell Biology and Regenerative Medicine
2024
University of California, San Diego
2020-2022
California State University, Fullerton
2019-2020
Hospital de Base
2008
Armed Forces Medical College
2008
The N6-methyladenosine (m 6 A) modification is the most prevalent post-transcriptional mRNA modification, regulating decay and splicing. It plays a major role during normal development, differentiation, disease progression. regulated by set of writer, eraser, reader proteins. YTH domain family proteins consists three homologous m A-binding proteins, Ythdf1, Ythdf2, Ythdf3, which were suggested to have different cellular functions. However, their sequence similarity tendency bind same targets...
Circular RNAs (circRNAs) are stable, often highly expressed RNA transcripts with potential to modulate other regulatory RNAs. A few circRNAs have been shown bind RNA-binding proteins (RBPs); however, little is known about the prevalence and distribution of these interactions in different biological contexts.We conduct an extensive screen circRNA-RBP ENCODE cell lines HepG2 K562. We profile deep-sequenced total samples analyze using a large set eCLIP data binding sites 150 RBPs. validate for...
RNA-binding proteins (RBPs) are critical regulators of post-transcriptional gene expression, and aberrant RBP-RNA interactions can promote cancer progression. Here, we interrogate the function RBPs in using pooled CRISPR-Cas9 screening identify 57 RBP candidates with distinct roles supporting MYC-driven oncogenic pathways. We find that disrupting YTHDF2-dependent mRNA degradation triggers apoptosis triple-negative breast (TNBC) cells tumors. eCLIP m6A sequencing reveal YTHDF2 interacts mRNAs...
Current perceptions of genetic and environmental vulnerabilities in the developing fetus are biased toward male outcomes. An argument is made that males more vulnerable to gestational complications neurodevelopmental disorders, implication being an understanding disrupted development sufficient understand causal mechanisms assumed be similar but attenuated females. Here we examine this assumption context immune-driven alterations fetal brain related outcomes female mice. Pregnant C57BL/6...
The circadian clock is encoded by a negative transcriptional feedback loop that coordinates physiology and behavior through molecular programs remain incompletely understood. Here, we reveal rhythmic genome-wide alternative splicing (AS) of pre-mRNAs encoding regulators peptidergic secretion within pancreatic β cells are perturbed in
Myotonic dystrophy type 1 (DM1) is a multisystem, autosomal-dominant inherited disorder caused by CTG microsatellite repeat expansions (MREs) in the 3′ untranslated region of dystrophia myotonica-protein kinase ( DMPK ) gene. Despite its prominence as most common adult-onset muscular dystrophy, patients with congenital to juvenile-onset forms DM1 can present debilitating neurocognitive symptoms along autism spectrum, characteristic possible utero cortical defects. However, molecular...
A rare incidence of multiple bacterial infections was reported in Litopenaeus vannamei grow-out ponds India. The pathogenic organisms identified are Aeromonas veronii, Bacillus flexus, Kurthia gibsonii, Shewanella decolorationis, cereus and amazonensis. infected shrimp showed various clinical morphological changes such as mucus secretion the hepatopancreas, spots carapace, red or pink discoloration abdomen with black stripes highly melanized body. Histological studies hepatopancreas revealed...
Abstract The N6-methyladenosine (m 6 A) modification is the most prevalent post-transcriptional mRNA modification, regulating decay, translation and splicing. It plays a major role during normal development, differentiation, disease progression. dynamically regulated by set of writer, eraser reader proteins. YTH-domain family proteins: Ythdf1, Ythdf2, Ythdf3, are three homologous m A binding proteins, which have different cellular functions. However, their sequence similarity tendency to...
Abstract Circular RNAs (circRNAs) are stable, often highly expressed RNA transcripts with potential to modulate other regulatory RNAs. A few circRNAs have been shown bind binding proteins (RBPs), however, little is known about the prevalence and strength of these interactions in different biological contexts. Here, we comprehensively evaluate interplay between RBPs ENCODE cell lines, HepG2 K562, by profiling expression fractionated total RNA-sequencing samples analyzing sites 150 large eCLIP...
ABSTRACT The biological mechanisms that sustain the vast blood production required for healthy life remain incompletely understood. To address this knowledge gap, we developed an in vivo hematopoietic stem cell (HSC)-based large-scale CRISPR knockout screening platform to enable genetic interrogation of hematopoiesis and broad aspects immune function vivo. Targeting ∼7000 genes with methodology, discovered SAGA complex members Tada2b Taf5l as key regulators HSC lineage commitment. Loss or...
Polypyrimidine tract binding protein 1 (PTBP1) is a well-studied RNA that serves as an important model for understanding molecular mechanisms underlying alternative splicing regulation. PTBP1 has four domains (RBDs) connected via linker regions. Additionally, N-terminal unstructured region contains nuclear import and export sequences. Each RBD can bind to pyrimidine rich elements with high affinity mediate activity. Studies support variety of models how regulation on target exons. Obtaining...