A5038615323- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- MicroRNA in disease regulation
- RNA Research and Splicing
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Chromatin Remodeling and Cancer
- Muscle Physiology and Disorders
- Cancer Mechanisms and Therapy
- Fungal and yeast genetics research
- 3D Printing in Biomedical Research
- Neurogenesis and neuroplasticity mechanisms
- Histone Deacetylase Inhibitors Research
- Pluripotent Stem Cells Research
- Amyotrophic Lateral Sclerosis Research
- Mechanisms of cancer metastasis
- RNA modifications and cancer
- Cancer-related gene regulation
The Graduate Center, CUNY
2020-2025
City University of New York
2020-2025
CUNY Advanced Science Research Center
2021-2025
Abstract The complexity of affected brain regions and cell types is a challenge for Huntington’s disease (HD) treatment. Here we use single nucleus RNA sequencing to investigate molecular pathology in the cortex striatum from R6/2 mice human HD post-mortem tissue. We identify type-specific -agnostic signatures suggesting oligodendrocytes (OLs) oligodendrocyte precursors (OPCs) are arrested intermediate maturation states. OL-lineage regulators OLIG1 OLIG2 negatively correlated with CAG length...
Amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) are incurable neurodegenerative disorders sharing pathological genetic features, including mutations in the FUS gene. is an RNA-binding protein that mislocalizes to cytoplasm aggregates ALS/FTD. In a yeast model, proteinopathy connected changes epigenome, reductions levels of H3S10ph, H3K14ac, H3K56ac. Exploiting same we reveal novel connections between aggregation epigenetic dysregulation. We show histone-modifying...
Adult oligodendrocyte progenitors (aOPCs) generate myelinating oligodendrocytes like neonatal (nOPCs), and they also display unique functional features. Here, using unbiased histone proteomics analysis ChIP sequencing of PDGFRα+ OPCs sorted from adult Pdgfra-H2B-EGFP reporter mice, we identify the activating H4K8ac mark as enriched in aOPCs. We detect increased occupancy at chromatin locations corresponding to genes related progenitor state (e.g., Hes5, Gpr17), metabolic processes Txnip,...
Abstract The N‐myc downstream regulated gene family member 1 ( NDRG1 ) is a whose mutation results in peripheral neuropathy with central manifestations. While most of previous studies characterized role Schwann cells, the detection nervous system symptoms and identification as silenced white matter multiple sclerosis brains raise question regarding its oligodendrocytes. Here, we show that enriched oligodendrocytes myelin preparations, characterize expression using novel reporter mouse...
ABSTRACT SUMMARY Dansu et al. identify distinct histone H4 modifications as potential mechanism underlying the functional differences between adult and neonatal progenitors. While H4K8ac favors expression of differentiation genes, their is halted by H4K20me3. Adult oligodendrocyte progenitors (aOPCs) generate myelinating oligodendrocytes, like (nOPCs), but they also display unique features. Here, using RNA-sequencing, unbiased proteomics analysis ChIP-sequencing, we define transcripts marks...
The protein arginine methyl transferase PRMT5 is an enzyme expressed in oligodendrocyte lineage cells and responsible for the symmetric methylation of residues on histone tails. Previous work from our laboratory identified as critical myelination, due to its transcriptional regulation genes involved survival early stages differentiation. However, besides nuclear localization, found at high levels cytoplasm several cell types, including progenitor (OPCs) yet, interacting partners this...
Metabolomics, the study to identify and quantify small molecules metabolites present in an experimental sample, has emerged as important tool investigate biological activities during development diseases. Metabolomics approaches are widely employed of cancer, nutrition/diet, diabetes, other physiological pathological conditions involving metabolic processes. An advantageous that aids metabolomic profiling advocated this paper is matrix-assisted laser desorption/ionization mass spectrometry...
Abstract The complexity of affected brain regions and cell types is a challenge for Huntington’s disease (HD) treatment. Here we used single nucleus RNA sequencing (snRNAseq) to investigate mechanism pathology in the cortex striatum from R6/2 mice at 8 12w three human HD post-mortem tissue. We identified type-specific agnostic signatures found changes suggesting oligodendrocytes (OLs) oligodendrocyte precursors (OPCs) were arrested intermediate maturation states. OL-lineage regulators OLIG1...
Metabolomics, the study to identify and quantify small molecules metabolites present in an experimental sample, has emerged as important tool investigate biological activities during development diseases. Metabolomics approaches are widely employed of cancer, nutrition/diet, diabetes, other physiological pathological conditions involving metabolic processes. An advantageous that aids metabolomic profiling advocated this paper is matrix-assisted laser desorption/ionization mass spectrometry...