A5100407083- Biochemical and Molecular Research
- HIV/AIDS drug development and treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- RNA Interference and Gene Delivery
- Mosquito-borne diseases and control
- Enzyme Production and Characterization
- Neuropeptides and Animal Physiology
- Advanced biosensing and bioanalysis techniques
- Computational Drug Discovery Methods
- DNA and Nucleic Acid Chemistry
- Enzyme Structure and Function
- Trypanosoma species research and implications
- Inflammatory mediators and NSAID effects
- Malaria Research and Control
- Marine Invertebrate Physiology and Ecology
- thermodynamics and calorimetric analyses
- Phytase and its Applications
- Drug Transport and Resistance Mechanisms
- Fractal and DNA sequence analysis
- Animal Genetics and Reproduction
- Machine Learning in Bioinformatics
- Protease and Inhibitor Mechanisms
- Synthesis and bioactivity of alkaloids
- Polyamine Metabolism and Applications
- Synthesis of heterocyclic compounds
MRC Laboratory of Molecular Biology
2025
University of Groningen
2018-2024
Peking University
2001-2024
Xihua University
2022
Jiangnan University
2019
Hefei University of Technology
2018
Qilu University of Technology
2017
Tianjin Medical University
2016
Changchun Institute of Applied Chemistry
2016
Chinese Academy of Sciences
2016
A novel intestinal “Trojan horse” for potential gene therapy of disorders.
The SARS-CoV-2 viral spike protein S receptor-binding domain (S-RBD) binds ACE2 on host cells to initiate molecular events, resulting in intracellular release of the genome. Therefore, antagonists this interaction could allow a modality for therapeutic intervention. Peptides can inhibit S-RBD:ACE2 by interacting with protein-protein interface. In study, contact atlas data and dynamics simulations were used locate hotspots secondary structure elements α1, α2, α3, β3, β4 ACE2. We designed...
Lipid derivatives of nucleoside analogs have been highlighted for their potential effective gene delivery. A novel class nucleobase-lipids are rationally designed and readily synthesized, comprising thymine/cytosine, an ester/amide linker oleyl lipid. The diversity four termed DXBAs (DOTA, DNTA, DOCA DNCA) is investigated. Besides, DNCA demonstrated to be neutral transfection material nucleic acid delivery, which enbles bind oligonucleotides via H-bonding π-π stacking with reduced toxicity...
To explore the epidemiological changes of Japanese encephalitis (JE) in a long-time span and evaluate impact mass vaccination. Data JE cases from hospitals county-level Centers for Disease Control Prevention Guizhou Province were collected between 2005 2021. The analyzed according to series policy implementation coronavirus disease 2019 (COVID-19). A total 5138 152 deaths reported during 2005–2021. average incidence case fatality rate 0.83/100,000 2.96%, respectively. prevalence demonstrated...
The discovery and development of new drugs against malaria remain urgent. Aspartate transcarbamoylase (ATC) has been suggested to be a promising target for antimalarial drug development. Here, we describe series small-molecule inhibitors P. falciparum ATC with low nanomolar binding affinities that selectively bind previously unreported allosteric pocket, thereby inhibiting activation. We demonstrate the buried pocket is located close traditional active site reported compounds maintain PfATC...
Aspartate transcarbamoylase catalyzes the second step of de-novo pyrimidine biosynthesis. As malarial parasites lack salvage machinery and rely on production for growth proliferation, this pathway is a target drug discovery. Previously, an apo crystal structure aspartate from Plasmodium falciparum (PfATC) in its T-state has been reported. Here we present structures PfATC liganded R-state as well complex with novel inhibitor, 2,3-napthalenediol, identified by high-throughput screening. Our...
A method of analyzing DNA correlation structure is introduced. Density fluctuations nucleotides are shown to display an extended self-similarity scaling when the scale varies between 100 and 8000 base pairs. The accurately described by a hierarchical model She Leveque [Phys. Rev. Lett. 72, 336 (1994)]. derived parameter $\ensuremath{\beta}$ able quantify moderately large-scale correlations which exist in true sequence but absent its randomly shuffled simulated evolution Hsieh et al. 90,...
N-(1-Deoxy-d-fructos-1-yl)-l-amino acids isolated from hog liver are endogenous lead decorporation substances with low toxicity and cell membrane crossing ability. To simulate the effect of natural N-(1-deoxy-d-fructos-1-yl)-l-amino on decorporation, a series epimerically pure (6a−eβ) were synthesized, their usefulness as antagonists intoxication was investigated. The results suggest that after treatment 6a−eβ liver, kidney, bone, brain, levels mice significantly reduced in comparison...
Abstract β-Ureidopropionase (βUP) deficiency is an autosomal recessive disease caused by abnormal changes in the pyrimidine-degradation pathway. This study aimed to investigate mutation of β-ureidopropionase gene ( UPB1 ) and clinical features 7 Chinese patients with βUP deficiency. We reported who were admitted at Tianjin Children's Hospital. Urine metabolomics was detected gas chromatography–mass spectrometry (GC–MS). Then genetic testing conducted polymerase chain reaction (PCR) method....
Aspartate transcarbamoylase (ATCase) plays a key role in the second step of de novo pyrimidine biosynthesis eukaryotes and has been proposed to be target suppress cell proliferation E. coli, human cells malarial parasite. We hypothesized that library ATCase inhibitors developed for (PfATCase) may also contain tubercular provide similar inhibition cellular proliferation. Of 70 compounds screened, 10 showed single-digit micromolar an vitro activity assay were tested their effect on M....
The appearance of multi-drug resistant strains malaria poses a major challenge to human health and validated drug targets are urgently required. To define protein's function in vivo thereby validate it as target, highly specific tools required that modify protein with minimal cross-reactivity. While modern genetic approaches often offer the desired level target specificity, applying these techniques is frequently challenging-particularly most dangerous parasite, Plasmodium falciparum. Our...
A novel and potentially active dihydroorotate dehydrogenase (DHODH) inhibitor, namely 3-({( E )-[( )-1-(biphenyl-4-yl)ethylidene]hydrazinylidene}methyl)-1 H -indole (BEHI) acetonitrile disolvate, C 23 19 N 3 ·2CH CN, has been designed synthesized. The structure of BEHI was characterized by elemental analysis, Q-TOF (quadrupole time-of-flight) MS, NMR, UV–Vis single-crystal X-ray diffraction. antitumour activity the target molecule evaluated MTT method. Results indicated that exhibited rather...