A5015467519- Immune Cell Function and Interaction
- Gut microbiota and health
- Drug Transport and Resistance Mechanisms
- IL-33, ST2, and ILC Pathways
- Clostridium difficile and Clostridium perfringens research
- Immune Response and Inflammation
- Parasites and Host Interactions
- Toxoplasma gondii Research Studies
- Parasitic Infections and Diagnostics
- Gastrointestinal motility and disorders
- Helicobacter pylori-related gastroenterology studies
- T-cell and B-cell Immunology
- Eosinophilic Esophagitis
- Parasitic infections in humans and animals
- Probiotics and Fermented Foods
- Herpesvirus Infections and Treatments
- Digestive system and related health
- Antimicrobial Peptides and Activities
- Diabetes and associated disorders
- Phagocytosis and Immune Regulation
- Whipple's Disease and Interleukins
- Inflammatory Bowel Disease
- Immunodeficiency and Autoimmune Disorders
- Estrogen and related hormone effects
- Immune cells in cancer
The University of Texas Health Science Center at Houston
2023-2025
Baylor College of Medicine
2019-2023
Texas Children's Hospital
2019-2022
The University of Texas Medical Branch at Galveston
2016-2020
German Institute for Disaster Medicine
2018
University of Tübingen
2011-2018
Jilin University
2011-2013
Bipar
2011
Innate lymphoid cells (ILCs) and CD4+ T produce IL-22, which is critical for intestinal immunity. The microbiota central to IL-22 production in the intestines; however, factors that regulate by ILCs are not clear. Here, we show microbiota-derived short-chain fatty acids (SCFAs) promote through G-protein receptor 41 (GPR41) inhibiting histone deacetylase (HDAC). SCFAs upregulate promoting aryl hydrocarbon (AhR) hypoxia-inducible factor 1α (HIF1α) expression, differentially regulated mTOR...
T-cells are crucial in maintanence of intestinal homeostasis, however, it is still unclear how microbiota metabolites regulate T-effector cells. Here we show gut microbiota-derived short-chain fatty acids (SCFAs) promote antigen-specific Th1 cell IL-10 production, mediated by G-protein coupled receptors 43 (GPR43). Microbiota Gpr43-/- CBir1 transgenic (Tg) cells, specific for antigen flagellin, induce more severe colitis compared with wide type (WT) Tg cells Rag-/- recipient mice. Treatment...
Intestinal IgA, which is regulated by gut microbiota, has a crucial role in maintenance of intestinal homeostasis and protecting the intestines from inflammation. However, means microbiota promotes IgA responses remain unclear. Emerging evidence suggests that host can sense bacterial metabolites addition to pathogen-associated molecular patterns recognition these small molecules influences immune response beyond. We reported here metabolite short-chain fatty acid acetate promoted responses,...
How the gut microbiota regulates intestinal homeostasis is not completely clear. Gut metabolite short-chain fatty acids (SCFAs) have been reported to regulate T-cell differentiation. However, mechanisms underlying SCFA regulation of differentiation and function remain be investigated.CBir1, an immunodominant antigen, transgenic T cells were treated with butyrate under various polarization conditions investigate mechanism involved. Transfer butyrate-treated CBir into Rag1-/- mice was...
The gut microbiota has been shown critical for mucosal adjuvant activity of cholera toxin (CT), a potent adjuvant. However, the mechanisms involved remain largely unknown. In this study, we report that depletion bacteria significantly decreased and systemic Ab responses in mice orally immunized with OVA CT. Feeding short-chain fatty acids (SCFAs) promoted elicited by CT, and, more importantly, rescued antibiotic-treated mice. addition, deficient GPR43, receptor SCFAs, showed impaired...
Neutrophils are the first responders to sites of inflammation when intestinal epithelial barrier is breached and gut microbiota invade. Despite current efforts in understanding role neutrophils homeostasis, complex interactions between cells (IECs) still not well characterized. In this study, we demonstrated that enhanced production amphiregulin (AREG), a member EGFR ligand family, by IECs, which promoted IEC function tissue repair. Depletion resulted more severe colitis mice because...
Background & AimsRegenerating islet-derived protein (REG3γ), an antimicrobial peptide, typically expressed by intestinal epithelial cells (IEC), plays crucial roles in homeostasis and controlling gut microbiota. However, the mechanisms that regulate IEC expression of REG3γ are still largely unclear. In this study, we investigated whether how interleukin (IL) 33, alarmin produced response to injury, regulates IEC, thus contributing homeostasis.MethodsIEC were isolated from wild-type IL33-/-...
Metabolic imbalance leading to inflammatory hypoxia and stabilization of hypoxia-inducible transcription factors (HIFs) is a hallmark bowel diseases. We hypothesize that HIF could be stabilized in CD4+ T cells during intestinal inflammation alter the functional responses via regulation microRNAs. Our assays reveal markedly increased cell-intrinsic protein experimental colitis. microRNA screen primary points us towards miR-29a our subsequent studies identify selective role for HIF-2α...
As a key regulator of metabolism and inflammation, the orphan nuclear hormone receptor, liver receptor homolog-1 (LRH-1), has potential as therapeutic target for diabetes, nonalcoholic fatty disease, inflammatory bowel diseases (IBD). Discovery LRH-1 modulators been difficult, in part due to tendency synthetic compounds bind unpredictably within lipophilic binding pocket. Using structure-guided approach, we exploited newly discovered polar interaction lock agonists consistent orientation....
Alveolar Echinococcosis (AE) caused by the cestode Echinococcus multilocularis , is a severe helminth infection of man, where unrestricted parasite growth will ultimately result in organ failure and fatality. The tissue-infiltrative larval metacestode limited efficacy available drugs complicate successful intervention AE; patients often need life-long medication, if possible, surgical resection affected tissues organs. Resistance to AE has been reported, but determinants which confer...
Abstract Background In rural sub-Saharan Africa, endemic populations are often infected concurrently with several intestinal and intravascular helminth protozoan parasites. A specific, balanced and, to an extent, protective immunity will develop over time in response repeated parasite encounters, immune responses initially being poorly adapted non-protective. The cellular production of pro-inflammatory regulatory cytokines chemokines helminth, antigens ubiquitous allergens were studied...
Phosphoinositide-dependent kinase-1 (PDK1) is a master kinase of the protein A, G, and C (AGC) family kinases that play important roles in regulating cancer cell proliferation, survival, metabolism. Besides phosphorylating/activating AKT at membrane PI3K-dependent manner, PDK1 also exhibits constitutive activity on many other AGC for tumor-promoting activity. In latter case, levels dominate its We previously reported MAPK4, an atypical MAPK, can PI3K-independently promote activation tumor...
Toxoplasmosis is a world-wide zoonosis that causes significant public health and veterinary problems. The study of vaccines remains the most promising method for future prevention control toxoplasmosis. Recombinant Toxoplasma gondii cyclophilin has been shown to have potent PPIase IL-12-inducing activities, thus promoting stabilization T. gondii's life cycle maintaining survival its host during evolution. In this study, gene was used construct DNA vaccine (pVAX1-TgCyP). immune response...
Chemokine and antibody response profiles were investigated in children adults with severe or uncomplicated Plasmodium falciparum malaria; the aim was to reveal which are associated disease, as often seen nonimmune children, mild semi-immune adults. Blood samples obtained from under 5 years of age well malaria. Classification malaria performed according parasite densities hemoglobin concentrations. Plasma levels chemokines (IL-8, IP-10, MCP-4, TARC, PARC, MIP-1δ, eotaxins) quantified,...
In sub-Saharan Africa poly-parasite infections are frequently observed in children, and with poly-parasitism modulating immune mechanisms, mediated by cytokines chemokines, required to prevent overwhelming inflammation host tissue damage. We analyzed children co-infected helminthes protozoan parasites their cellular production of regulatory pro-inflammatory chemokines response parasite antigens allergens.Intestinal intravascular were detected stool urines samples. The vitro cytokine...
The role of IL-21, produced mainly by Th17 cells and T follicular helper cells, has been intensively investigated in B cell differentiation Ab class switch. However, how IL-21 regulates memory IgA+ development IgA responses the intestines is still not completely understood. In this study, we found total as well CD38+CD138-IgA+ were significantly increased intestinal lamina propria (LP) TCRβxδ-/- mice after transfer microbiota Ag-specific but Th1 cells. Although IL-21R-/- or IL-17R-/- showed...
// Xiangsheng Huang 1, * , Stefan Wiehr 2, Anna-Maria Wild 2 Patrick Voßberg 1 Wolfgang Hoffmann Beate Grüner 3 Carsten Köhler and Peter T. Soboslay Institute for Tropical Medicine, Eberhard Karls University, Tübingen, Germany Werner Siemens Imaging Center, Department of Preclinical Radiopharmacy, Section Clinical Immunology Infectiology, University Clinics Ulm, These authors contributed equally to this work Correspondence to: Soboslay, email: peter.soboslay@uni-tuebingen.de Keywords:...