A5010689909- Bacterial biofilms and quorum sensing
- Escherichia coli research studies
- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- Urinary Tract Infections Management
- Bacteriophages and microbial interactions
- Antimicrobial Peptides and Activities
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Marine Biology and Environmental Chemistry
- Oral microbiology and periodontitis research
- Vibrio bacteria research studies
- Advanced Sensor and Energy Harvesting Materials
- Antimicrobial Resistance in Staphylococcus
- Pediatric Urology and Nephrology Studies
- Oral Health Pathology and Treatment
- Polysaccharides and Plant Cell Walls
- Mycobacterium research and diagnosis
- Biochemical and Structural Characterization
- Polymer Surface Interaction Studies
- Enterobacteriaceae and Cronobacter Research
- Corrosion Behavior and Inhibition
- Diffusion and Search Dynamics
- Phytoestrogen effects and research
- Silk-based biomaterials and applications
University of Copenhagen
2014-2021
Technical University of Denmark
2005-2012
ABSTRACT Escherichia coli strains are responsible for many cases of gastrointestinal disease and represent a serious health problem worldwide. An essential step in the pathogenesis such involves recognition attachment to host intestinal surfaces. TibA is potent bacterial adhesin associated with number enterotoxigenic E. mediates variety human cells; additionally, it promotes invasion cells. This surface-displayed autotransporter protein belongs exclusive group glycoproteins; only...
Abstract For the last decade, chemical control of bacterial virulence has received considerable attention. Ajoene, a sulfur-rich molecule from garlic been shown to reduce expression key quorum sensing regulated factors in opportunistic pathogen Pseudomonas aeruginosa . Here we show that repressing effect ajoene on occurs by inhibition small regulatory RNAs (sRNA) P as well Staphylococcus aureus , another important human employs gene expression. Using various reporter constructs, found...
Glycosylation is a common modulation of protein function in eukaryotes and biologically important. However, bacteria glycosylation rare, relatively few bacterial glycoproteins are known. In Escherichia coli only two have been described to date. Here we introduce novel member this exclusive group, namely, antigen 43 (Ag43), self-recognizing autotransporter protein. By mass spectrometry Ag43 was demonstrated be glycosylated by addition heptose residues at several positions the passenger...
ABSTRACT The virulence determinants of uropathogenic Escherichia coli have been studied extensively over the years, but relatively little is known about what differentiates isolates causing various types urinary tract infections. In this study, we compared genomic profiles 45 strains from a range different clinical backgrounds, i.e., urosepsis, pyelonephritis, cystitis, and asymptomatic bacteriuria (ABU), using comparative hybridization analysis. A microarray based on 31 complete E....
Abstract Background Inflammatory bowel disease (IBD) is used to describe a state of idiopathic, chronic inflammation the gastrointestinal tract. The two main phenotypes IBD are Crohn's (CD) and ulcerative colitis (UC). major cause IBD-associated mortality colorectal cancer. Although both host-genetic exogenous factors have been found be involved, aetiology still not well understood. In this study we characterized thirteen Escherichia coli strains from patients with by comparative genomic...
ABSTRACT Escherichia coli is the most important etiological agent of urinary tract infections (UTIs). Unlike uropathogenic E. , which causes symptomatic infections, asymptomatic bacteriuria (ABU) strains typically lack essential virulence factors and colonize bladder in absence symptoms. While ABU can persist for long periods time, little known about genetic determinants required its growth fitness urine. To identify such genes, we have employed a transposon mutagenesis approach using...
ABSTRACT Food is now recognized as a natural resource of novel antimicrobial agents, including those that target the virulence mechanisms bacterial pathogens. Iberin, an isothiocyanate compound from horseradish, was recently identified quorum-sensing inhibitor (QSI) pathogen Pseudomonas aeruginosa . In this study, we used comparative systems biology approach to unravel molecular effects iberin on QS and factor expression P. Our study shows two methods (i.e., RNA sequencing proteomics)...
In this study we report on a novel structural phenotype in Escherichia coli biofilms: cellular chain formation. Biofilm chaining E. K-12 was found to occur primarily by clonal expansion, but not due filamentous growth. Rather, formation the result of intercellular interactions facilitated antigen 43 (Ag43), self-associating autotransporter (SAAT) protein, which has previously been implicated auto-aggregation and biofilm Immunofluorescence microscopy suggested that Ag43 concentrated at or...
Preconditioning of stainless steel with aqueous cod muscle extract significantly impedes subsequent bacterial adhesion most likely due to repelling effects fish tropomyosin. The purpose this study was determine if other food conditioning films decrease or enhance steel.Attachment Pseudomonas fluorescens AH2 coated water-soluble coatings animal origin reduced as compared noncoated laboratory substrate extracts plant origin. Coating also decreases food-relevant bacteria. manipulation not...
From microbial biofilms to human migrations, spatial competition is central the evolutionary history of many species. The boundary between expanding populations focal point for space and resources particular interest in ecology. For all Escherichia coli strains studied here, these boundaries move a counterclockwise direction even when competing have same fitness. We find that chiral growth bacterial colonies strongly suppressed by expression extracellular features such as adhesive structures...
Urinary tract infections (UTIs) are among the most common infectious diseases of humans, with Escherichia coli being responsible for >80% all cases. Asymptomatic bacteriuria (ABU) occurs when bacteria colonize urinary without causing clinical symptoms and can affect both catheterized patients (catheter-associated ABU [CA-ABU]) noncatheterized patients. Here, we compared virulence properties a collection CA-ABU nosocomial E. isolates in terms antibiotic resistance, phylogenetic grouping,...
Extracellular DNA (eDNA) is a major constituent of the extracellular matrix Pseudomonas aeruginosa biofilms and its release regulated via pseudomonas quinolone signal (PQS) dependent quorum sensing (QS). By screening P. transposon library to identify factors required for release, mutants with insertions in twin-arginine translocation (Tat) pathway were identified as exhibiting reduced eDNA defective biofilm architecture enhanced susceptibility tobramycin. tat showed substantial reductions...
ABSTRACT Microbial adhesion and biofilm formation on surfaces pose major problems risks to human health. One way circumvent this problem is coat (in report stainless steel) with a non-toxic fish extract that generates an abiotic surface less bacterial attachment than uncoated or coated with, for example, tryptone soy broth. The bacteria grow well in the extract; hence general bacteriocidal effect not reason antifouling effect. Bacterial was quantified by different methods including (a)...
Bacterial biofilm formation on inert surfaces is a significant health and economic problem in wide range of environmental, industrial, medical areas. adhesion generally prerequisite for this colonization process and, thus, represents an attractive target the development biofilm-preventive measures. We have previously found that preconditioning several different materials with aqueous fish muscle extract, composed primarily alpha-tropomyosin, significantly discourages bacterial attachment to...
We have recently found that preconditioning of stainless steel surfaces with an aqueous fish muscle extract can significantly impede bacterial adhesion. The purpose this study was to identify and characterize the primary components associated bacteria-repelling effect.The anti-adhesive activity assayed against Escherchia coli K-12, adhesion quantified by crystal violet staining sonication methods. Proteolytic digestion, elution fractionation experiments revealed linked formation a...
Abstract Extracellular DNA (eDNA) is a major constituent of the extracellular matrix Pseudomonas aeruginosa biofilms and its release regulated via pseudomonas quinolone signal (PQS) dependent quorum sensing (QS). By screening P. transposon library to identify factors required for release, mutants with insertions in twin-arginine translocation (Tat) pathway were identified as exhibiting reduced eDNA defective biofilm architecture enhanced susceptibility tobramycin. tat showed substantial...