A5084066716- Neonatal Respiratory Health Research
- Respiratory Support and Mechanisms
- Neuroscience of respiration and sleep
- Airway Management and Intubation Techniques
- Developmental Biology and Gene Regulation
- Bacteriophages and microbial interactions
- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Congenital Diaphragmatic Hernia Studies
- Genetic Associations and Epidemiology
- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Mechanical Circulatory Support Devices
- Infant Development and Preterm Care
- Monoclonal and Polyclonal Antibodies Research
- Metabolism, Diabetes, and Cancer
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Mass Spectrometry Techniques and Applications
- RNA Interference and Gene Delivery
- Diabetes, Cardiovascular Risks, and Lipoproteins
University of Washington
2008-2025
Duke University Hospital
2024-2025
Duke University
2020-2025
Duke Medical Center
2024-2025
Living Systems (United States)
2025
Seattle Children's Hospital
2008-2020
University of Utah
1978
Library screening and selection methods can determine the binding activities of individual members large protein libraries given a physical link between nucleotide sequence, which enables identification functional molecules by DNA sequencing. However, solution properties cannot be probed using such approaches because they are completely altered attachment. Mass spectrometry parallel evaluation amenable to fractionation as solubility oligomeric state, but current limited 1,000 or fewer...
Elongation of the vertebrate embryonic axis necessitates rapid expansion epidermis to accommodate growth underlying tissues. Here, we generated a toolkit visualize and quantify signaling in entire cell populations periderm, outermost layer epidermis, live developing zebrafish. We find that oriented divisions facilitate early periderm during axial elongation rather than addition from basal layer. Activity levels Extracellular signal-regulated kinase (ERK), downstream effector MAPK pathway,...
<h3>BACKGROUND:</h3> Bubble CPAP is frequently used in spontaneously breathing infants with lung disease. Often bubble systems lack pressure alarms and pressure-release valves. We observed a large volume of condensate the exhalation limb patient circuit conducted series experiments to test hypothesis that accumulated could affect delivered pressures. <h3>METHODS:</h3> An anatomically accurate nasal airway model preterm infant was attached model. A system bi-nasal short prongs, rate fluid...
<h3>BACKGROUND:</h3> High-frequency pressure oscillations created by gas bubbling through an underwater seal during bubble CPAP may enhance ventilation and aid in lung recruitment premature infants. We hypothesized that there are no differences the magnitude of volume (ΔV) a preterm neonatal model when different systems used. <h3>METHODS:</h3> An anatomically realistic replica infant nasal airway was attached to Silastic test sealed within calibrated plethysmograph. Nasal prongs were affixed...
Background Almost one million prematurely born infants die annually from respiratory insufficiency, predominantly in countries with limited access to support for neonates. The primary hypothesis tested the present study was that a modified device bubble nasal continuous positive airway pressure (Bn-CPAP) would provide lower work of spontaneous breathing, estimated by esophageal pressure-rate products. Methods Infants <32 weeks gestation and stable on Bn-CPAP FiO2 <0.30 were studied within 72...
Controlling the biodistribution of protein- and nanoparticle-based therapeutic formulations remains challenging. In vivo library selection is an effective method for identifying constructs that exhibit desired distribution behavior; variants can be selected based on their ability to localize tissue or compartment interest despite complex physiological challenges. Here, we describe further development in platform self-assembling protein nanoparticles encapsulating own mRNA genomes (synthetic...
Abstract Objective To test the hypothesis that infants born <30 weeks’ gestation supported by Seattle-PAP will have lower rates of continuous positive airway pressure (CPAP) failure than with conventional, Fisher&Paykel-CPAP (FP-CPAP). Study design Randomized trial (3/2017-01/2019) at 5 NICUs. The primary outcome was CPAP failure; subgroup analyses (gestational age, receipt antenatal corticosteroids) were performed. Results A total 232 randomized. Infants in and FP-CPAP groups had...
The frequent use of continuous positive airway pressure and end-expiratory in newborn infants with pulmonary disease has prevented the conventional methods for measuring oxygen uptake (VO2) intensive-care units. A feed-back replenishment technique determination these been developed. An instrument utilizing this method designed built permitting VO2 monitoring without interfering routine ventilatory therapy critically ill infant. Theoretical, bench, animal experiments using room air as an...
Abstract Metabolic syndrome (MetS) is multifaceted. Risk factors include visceral adiposity, dyslipidemia, hyperglycemia, hypertension and environmental stimuli. MetS leads to an increased risk of cardiovascular disease, type 2 diabetes stroke. Comparative studies, however, have identified heterogeneity in the pathology across groups though etiology these differences has yet be elucidated. The Syndrome Research Resource (MetSRR) described this report a curated database that provides access...
Elongation of the vertebrate embryonic axis necessitates rapid expansion epidermis to accommodate growth underlying tissues. Here, we generated a toolkit visualize and quantify signaling in entire cell populations periderm, outermost layer epidermis, live developing zebrafish. We find that oriented divisions facilitate early periderm during axial elongation rather than addition from basal layer. Activity levels ERK, downstream effector MAPK pathway, gauged by biosensor, predicts cycle entry,...