- Milk Quality and Mastitis in Dairy Cows
- Probiotics and Fermented Foods
- Microbial infections and disease research
- Streptococcal Infections and Treatments
- Neonatal and Maternal Infections
- Animal Behavior and Welfare Studies
- Infant Nutrition and Health
- Bacteriophages and microbial interactions
- Animal Genetics and Reproduction
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Enterobacteriaceae and Cronobacter Research
- Genetic and phenotypic traits in livestock
- Meat and Animal Product Quality
- Vector-borne infectious diseases
- Effects of Environmental Stressors on Livestock
- Microbial Metabolism and Applications
- Transgenic Plants and Applications
- Enzyme Production and Characterization
- Antimicrobial Resistance in Staphylococcus
- Infective Endocarditis Diagnosis and Management
- Fungal Biology and Applications
- Respiratory viral infections research
- Agriculture and Farm Safety
- Viral gastroenteritis research and epidemiology
DNA Diagnostic (Denmark)
2015-2021
Knowledge Centre for Agriculture
2011-2015
Sygehus Sønderjylland
2006
Results of a commercial real-time PCR analysis for 11 mastitis pathogens from bulk tank milk (BTM) samples all 4,258 Danish dairy herds in November 2009 to January 2010 were compared with somatic cell count (SCC) and total bacteria (TBC) estimates BTM. For Streptococcus agalactiae, dysgalactiae, uberis, low cycle threshold (Ct) value (corresponding high bacterial DNA quantity) was correlated higher SCC TBC. Staphylococcus aureus, Ct values only SCC. the environmental Klebsiella spp.,...
Streptococcus agalactiae is an emerging pathogen of nonpregnant human adults worldwide and a reemerging dairy cattle in parts Europe. To learn more about interspecies transmission this bacterium, we compared contemporaneously collected isolates from humans Finland Sweden. Multilocus sequence typing identified 5 types (STs) (ST1, 8, 12, 23, 196) shared across the 2 host species, suggesting possible transmission. More than 54% belonged to those STs. Molecular serotyping pilus island did not...
Abstract Intramammary infections (IMI) with Staphylococcus aureus are a common cause of bovine mastitis and can result in both clinical (CM) or subclinical (SCM). Although bacterial isolates S. differ their virulence potential it is largely unclear which factors responsible for increased severity. We performed genome wide association study used generalized linear mixed model to investigate the correlation between gene carriage, lineage outcome IMI collection from cattle CM (n = 125) SCM 151)...
Evidence for the efficacy of antimicrobial treatments in Escherichia coli mastitis is limited. The aim this double-blinded field trial was to investigate enrofloxacin compared with placebo, a special focus on survival, dairy cows acute clinical caused by E. coli. Dairy (n=116) were included study. A examination performed and milk sample from affected udder quarter collected investigation somatic cell count (SCC) bacteriology first day treatment (day 0) at 3 (clinical only), 22 28. Data...
Streptococcus agalactiae (Strep. agalactiae) and Staphylococcus aureus (Staph. aureus) are originally regarded as contagious mastitis pathogens, however, both pathogens have recently been isolated from extramammary environmental sites, indicating that other sites than the udder might contribute to spread of these potentially causing intramammary infections. Diagnostic tools identify at available but still needs be validated. The objective this cross-sectional field study was estimate...
Milk quality in bulk tank milk (BTM) is measured by flow cytometry technology as total bacterial count (TBC) and somatic cell (SCC). To investigate SCC problems, culture or PCR can be used to identify mastitis causing bacteria, e.g., Mastit 4, a commercially available qPCR test. TBC BTM investigated further using culture-based methods such standard plate count, laboratory pasteurization coliform spore counts. our knowledge, no addressing the bacteria involved has been introduced. The aim of...
Background Coliform mastitis is an acute and potentially lethal type of in bovine practice. The therapeutical approach to coliform frequently discussed. While generally accepted that supportive therapy, such as fluid necessary, the administration antibiotics questionned. This due mainly two reasons. Firstly, clinical ill effects observed are often thought be effect accompanying endotoxicosis, which would not influenced positively by killing non-invasive E. coli. Secondly, effective against...
To primarily estimate the sensitivity (Se) and specificity (Sp) of commercially available Mastit4 quantitative PCR (qPCR) assay bacterial culture (BC) for diagnosis intramammary infections (IMI) teat apex colonization (TAC) with coagulase‐negative staphylococci (CNS) at different cut‐offs qPCR cycle threshold values using Bayesian latent class analysis. A secondary objective was to evaluate two BC IMI TAC CNS. We randomly selected 13–20 cows subclinical mastitis from eight dairy herds. Teat...