A5084233509- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- DNA Repair Mechanisms
- Hepatocellular Carcinoma Treatment and Prognosis
- PARP inhibition in cancer therapy
- Advanced Chemical Physics Studies
- Hepatitis B Virus Studies
- Monoclonal and Polyclonal Antibodies Research
- Liver Disease Diagnosis and Treatment
- Liver physiology and pathology
- Molecular Junctions and Nanostructures
- Inorganic and Organometallic Chemistry
- Mitochondrial Function and Pathology
- Metal complexes synthesis and properties
- Biochemical and Molecular Research
- ATP Synthase and ATPases Research
- Metabolism, Diabetes, and Cancer
- Cancer Treatment and Pharmacology
- Biopolymer Synthesis and Applications
- Protein purification and stability
- CRISPR and Genetic Engineering
- Hepatitis C virus research
- Mass Spectrometry Techniques and Applications
- Organic Chemistry Cycloaddition Reactions
- Cancer Research and Treatments
University of Bologna
2016-2025
Dublin City University
2024
Italian Institute of Technology
2024
GNA University
2024
National Research Council
2024
Medizinische Hochschule Hannover
2021
University of Catania
2009
Azienda Ospedaliero-Universitaria Policlinico - Vittorio Emanuele
2009
Western General Hospital
2009
University of Edinburgh
2009
One of the most prominent alterations in cancer cells is their strict dependence on glycolytic pathway for ATP generation. This observation led to evaluation glycolysis inhibitors as potential anticancer agents. The inhibition lactate dehydrogenase (LDH) a promising way inhibit tumor cell glucose metabolism without affecting energetic balance normal tissues. However, success this approach depends chiefly availability that display good selectivity. We identified compound (galloflavin, CAS...
The sialyltransferase activities of 10 human colorectal specimens were compared with those the corresponding adjacent normal mucosa. Using asialofetuin as an acceptor we found, in tumor tissues 9 out patients, increased activity towards N-linked chains determined upon peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase (PNGase) treatment. On contrary, O-linked was not significantly changed. When specificity acting on investigated by using N-acetyl-lactosamine (Gal beta 1,4GlcNAc)...
Synthetic lethality is an innovative framework for discovering novel anticancer drug candidates. One example the use of PARP inhibitors (PARPi) in oncology patients with BRCA mutations. Here, we exploit a new paradigm based on possibility triggering synthetic using only small organic molecules (dubbed "fully small-molecule-induced lethality"). We exploited this to target pancreatic cancer, one major unmet needs oncology. discovered dihydroquinolone pyrazoline-based molecule (35d) that...
The expression of the asialoglycoprotein receptor (ASGP-R) on human hepatocellular carcinoma (HCC) cells might be exploited to reduce extrahepatic toxicity DNA synthesis inhibitors by their conjugation with galactosyl-terminating peptides. In present study we first assessed frequency ASGP-R in 60 HCCs. Secondly, investigated whether was maintained plasma membranes synthesizing cancer cells. Needle biopsies HCC were evaluated. Diagnosis and grading performed routine haematoxylin eosin-stained...
<i>Background/Aims:</i>By reducing the number of ATP molecules produced via aerobic glycolysis, inhibition lactic dehydrogenase (LDH) should hinder growth neoplastic cells without damaging normal which do not rely on this metabolic pathway for their energetic needs. Here, we studied effect oxamic and tartronic acids, 2 inhibitors LDH, glycolysis cell replication HepG2 PLC/PRF/5 cells, lines from human hepatocellular carcinomas. <i>Methods:</i>Aerobic was measured by...
Activation of the myc oncogene in cancer cells upregulates lactate dehydrogenase A (LDH-A) expression, leading to a sustained glycolytic flux that is needed produce ATP under hypoxic conditions. We studied effects galloflavin (GF), recently identified LDH inhibitor, on overexpressing Burkitt lymphoma (BL) cells. Epstein-Barr virus-infected lymphoblasts were used as non-neoplastic control. Our results showed overactivation induced two- seven-fold increase LDH-A expression BL compared with...
Abstract The RAD51-BRCA2 interaction is central to DNA repair through homologous recombination. Emerging evidence indicates RAD51 overexpression and its correlation with chemoresistance in various cancers, suggesting inhibition as a compelling avenue for intervention. We previously showed that combining olaparib (a PARP inhibitor (PARPi)) RS -35d BRCA2-RAD51 inhibitor) was efficient killing pancreatic ductal adenocarcinoma (PDAC) cells. However, impaired cell viability even when administered...
beta 1,4-N-Acetylgalactosaminyltransferase (beta 1,4GalNAc-transferase) and alpha 2,3-sialyltransferase are both involved in the biosynthesis of Sda blood group antigen, which is also present cells large intestine. The expression these enzymes 2,6-sialyltransferase activity towards N-acetyl-lactosamine was investigated rat intestinal correlated with cell differentiation extent postnatal maturation. 1,4GalNAc-transferase exclusively found epithelial intestine, preferentially proximal segments...
In BRCA2-defective cells, poly(adenosine diphosphate [ADP]-ribose) polymerase inhibitors can trigger synthetic lethality, as two independent DNA-repairing mechanisms are simultaneously impaired. Here, we have pharmacologically induced which was triggered by combining different small organic molecules. When administered with a BRCA2–Rad51 disruptor in nonmutant Olaparib showed anticancer activity comparable to that shown when alone cells. This strategy could represent an innovative approach...
Intrinsic or acquired drug resistance is one of the major problems compromising success antineoplastic treatments. Several evidences correlated some therapeutic failures with changes in cell metabolic asset and line these findings, hindering glycolytic metabolism cancer cells via lactate dehydrogenase (LDH) inhibition was found to overcome chemotherapeutic agents. Lactate, product LDH reaction, shown be involved epigenetic regulation gene expression. The experiments described this paper were...
The BRCA2-RAD51 interaction remains an intriguing target for cancer drug discovery due to its vital role in DNA damage repair mechanisms, which cells become particularly reliant on. Moreover, RAD51 has many synthetically lethal partners, including PARP1-2, can be exploited induce synthetic lethality cancer. In this study, we established a
Abstract The ESCA spectra of several substituted benzenes and heterocyclic compounds were obtained. Multi peak structures are observed in the various energy regions. effects generally interpreted as due to shake up phenomena involving intramolecular charge-transfer transitions between a "donor" an "acceptor" group molecule. A quantitative model for such kind is given. It rationalizes fact that: 1) These processes are, large extent, specific atoms acceptor groups; 2) energies found donor part...