A5065576253- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant Pathogens and Fungal Diseases
- Plant Physiology and Cultivation Studies
- Berry genetics and cultivation research
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Horticultural and Viticultural Research
- Helicobacter pylori-related gastroenterology studies
- Plant Pathogens and Resistance
- Plant Molecular Biology Research
- Fungal Plant Pathogen Control
- Reproductive biology and impacts on aquatic species
- Plant and animal studies
- Gastric Cancer Management and Outcomes
- Veterinary medicine and infectious diseases
- Eosinophilic Esophagitis
- Chromosomal and Genetic Variations
- Botanical Studies and Applications
- Plant biochemistry and biosynthesis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant-Microbe Interactions and Immunity
- CRISPR and Genetic Engineering
- Plant and Fungal Species Descriptions
- Gastroesophageal reflux and treatments
Plant & Food Research
2019-2025
Institute of Agrifood Research and Technology
2017-2022
James Hutton Institute
2017-2019
Hospital General Universitario de Elche
2012-2015
Consejo Superior de Investigaciones Científicas
2014
East Malling Research (United Kingdom)
2009-2012
The International Strawberry Sequencing Consortium reports the draft genome of woodland strawberry (Fragaria vesca). this diploid species should serve as a reference for Fragaria genus, cultivated × ananassa) is an octoploid where F. vesca predicted to be subgenome donor. strawberry, (2n = 2x 14), versatile experimental plant system. This diminutive herbaceous perennial has small (240 Mb), amenable genetic transformation and shares substantial sequence identity with other economically...
Abstract Background Comparative genome mapping studies in Rosaceae have been conducted until now by aligning genetic maps within the same genus, or closely related genera and using a limited number of common markers. The growing body genomics resources sequence data for both Prunus Fragaria permits detailed comparisons between these recently released Malus × domestica sequence. Results We generated comparative analysis 806 molecular markers that are anchored genetically to and/or reference...
A high-quality genome sequence of any model organism is an essential starting point for genetic and other studies. Older clone-based methods are slow expensive, whereas faster, cheaper short-read-only assemblies can be incomplete highly fragmented, which minimizes their usefulness. The last few years have seen the introduction many new technologies assembly. These associated algorithms typically benchmarked on microbial genomes or, if they scale appropriately, larger (e.g., human) genomes....
The aim of this study was to determine the diagnostic usefulness quantification H. pylori genome in detection infection patients with upper gastrointestinal bleeding (UGB). A total 158 consecutive digestive disorders, 80 whom had clinical presentation UGB, were studied. number microorganisms quantified using a real-time PCR system which amplifies urease gene an internal control for eliminating false negatives. biopsy sample from antrum and corpus each patient processed. rapid test, culture,...
Genomic methods for identifying causative variants trait loci applicable to a wide range of germplasm are required plant biologists and breeders understand the genetic control variation.
We assembled the genome of Leptospermum scoparium 'Crimson Glory' using a combination Illumina paired-end sequencing, high-throughput chromosome conformation capture (Hi-C) and high density genetic mapping. As is variety mānuka, this first assembly for plant species culturally recognised as treasure (taonga) by indigenous Māori Aotearoa New Zealand. The mānuka spans total 297 Mbp organised in 11 pseudo-chromosomes that are syntenic with Eucalyptus genome. A large proportion corresponds to...
Abstract Self-incompatibility (SI) in plants has evolved independently multiple times and S -RNase-based gametophytic self-incompatibility (GSI) is most common. The Rosaceae family possesses both self-recognition ( Prunus ) nonself-recognition Malus GSI systems, the latter widespread flowering plants. Gillenia trifoliata a species related to , providing utility for understanding SI evolution. sister taxon but unlike not undergone polyploidisation. In addition, common ancestor of close origin...
In peach, the flat phenotype is caused by a partially dominant allele in heterozygosis (Ss), fruits from homozygous trees (SS) abort few weeks after fruit setting. Previous research has identified SSR marker (UDP98-412) highly associated with trait, found suitable for assisted selection (MAS). Here we report ∼10 Kb deletion affecting gene PRUPE.6G281100, 400 upstream of UDP98-412, co-segregating trait. This leucine-rich repeat receptor-like kinase (LRR-RLK) orthologous to Brassinosteroid...
The genome sequence of the woodland strawberry (Fragaria vesca L.) is an important resource providing a reference for comparative genomics studies and future sequenced rosaceous species has great utility as model development markers mapping in cultivated Fragaria ×ananassa Duchesne ex Rozier. A set 152 microsatellite simple repeat (SSR) primer pairs was developed mapped, along with 42 previously published but unmapped SSRs, permitting precise assignment 28.2 Mbp unanchored scaffolds (13% F....
Epidemics of obesity and type 2 diabetes drive strong consumer interest in plant-based low-calorie sweeteners. Trilobatin is a sweetener found at high concentrations the leaves range crabapple (Malus) species, but not domesticated apple (Malus × domestica) leaves, which contain trilobatin's bitter positional isomer phloridzin. Variation trilobatin content was mapped to locus on LG 7 segregating population developed from cross between apples crabapples. Phloretin glycosyltransferase2 (PGT2)...
Genome complexity is largely linked to diversification and crop innovation. Examples of regions with duplicated genes relevant roles in agricultural traits are found many crops. In both non-duplicated genes, much the variability agronomic caused by large as well small middle scale structural variants (SVs), which highlights relevance identification characterization complex between genomes for plant breeding.Here we improve demonstrate use CRISPR-Cas9 enrichment combined long-read sequencing...
Background and Aim Several biological epidemiological studies support a relationship between smoking Helicobacter pylori (H. pylori) to increase the risk of pathology. However, there have been few on potential synergistic association specific cagA vacA virulence factors in patients infected by pylori. We studied cagA, i1 bacterial load H. patients. Methods Biopsies gastric corpus antrum from 155 consecutive whom was clinical suspicion infection were processed. In 106 detected. Molecular...
Abstract Background The genetic control of sex determination in teleost species is poorly understood. This partly because the diversity mechanisms that determine this large group vertebrates, including constitutive genes linked to chromosomes, polygenic mechanisms, environmental factors, hermaphroditism, and unisexuality. Here we use a de novo genome assembly New Zealand silver trevally ( Pseudocaranx georgianus ) together with sex-specific whole sequencing data detect sexually divergent...
A high quality genome sequence of your model organism is an essential starting point for many studies. Old clone based methods are slow and expensive, whereas faster, cheaper short read only assemblies can be incomplete highly fragmented, which minimises their usefulness. The last few years have seen the introduction new technologies assembly. These algorithms typically benchmarked on microbial genomes or, if they scale appropriately, human. However, plant much more repetitive larger than...
Abstract Background The genetic control of sex determinism in teleost species is poorly understood. This partly because the diversity determining mechanisms this large group, including constitutive genes linked to chromosomes, polygenic mechanisms, environmental factors, hermaphroditism, and unisexuality. Here we use a de novo genome assembly New Zealand silver trevally ( Pseudocaranx georgianus ) together with whole sequencing detect sexually divergent regions, identify candidate develop...
The cultivated strawberry Fragaria xananassa is one of the most economically-important soft-fruit species. Few structural genomic resources have been reported for and there exists an urgent need development physical mapping genus. first stage in a map construction characterisation high molecular weight bacterial artificial chromosome (BAC) library.A BAC library, consisting 18,432 clones was constructed from vesca f. semperflorens accession 'Ali Baba'. DNA individual library pooled to create...
Knowledge of the genotypes for self-incompatibility locus (<i>S</i>-locus) in apple varieties and being used as parents is critical breeding commercial production. We present a high-throughput set molecular markers identification 13 common <i>S-RN</i>ase alleles (<i>S</i><sub>1</sub>, <i>S</i><sub>2</sub>, <i>S</i><sub>3</sub>, <i>S</i><sub>5</sub>, <i>S</i><sub>7</sub>, <i>S</i><sub>8</sub>, <i>S</i><sub>9</sub>, <i>S</i><sub>10</sub>, <i>S</i><sub>20</sub>, <i>S</i><sub>23</sub>,...
ABSTRACT Genome complexity is largely linked to diversification and crop innovation. Examples of regions with duplicated genes relevant roles in agricultural traits are found many crops. In both non-duplicated genes, much the variability agronomic caused by large as well small middle scale structural variants (SVs), which highlights relevance identification characterization complex between genomes for plant breeding. Here we improve demonstrate use CRISPR-Cas9 enrichment combined long-read...