Left-right (LR) asymmetry is ubiquitous in animal development. Cytoskeletal chirality was recently reported to specify LR embryogenesis, suggesting that tissue morphogenesis coordinated by single- or multi-cell organizers. Thus, organize at multiscale levels of morphogenesis, cells with must also be present adequate numbers. However, observation rarely cultured cells.

Abstract Malignant melanoma (MM) is the most aggressive form of skin cancer. The delay in treatment will induce metastasis, resulting a poor prognosis and even death. Here, two‐step strategy for on‐site diagnosis MM developed based on extraction direct visual quantification S100A1, biomarker melanoma. First, swellable microneedle utilized to extract S100A1 interstitial fluid (ISF) with minimal invasion. After elution, antibody‐conjugated magnetic microparticles (MMPs) polystyrene (PMPs) are...

Abstract High-throughput laser micro-machining demands precise control of the beam position to achieve optimal efficiency, but existing methods can be both time-consuming and cost-prohibitive. In this paper, we demonstrate a new high-throughput technique based on rapidly scanning focal point along optical axis using an acoustically driven variable length lens. Our results show that method enables higher machining rates over range defocus distances effect becomes more significant as energy is...

Lead contamination in drinking water is a primary concern public health, but it difficult to monitor by end-users. Here, we provide rapid and power-free microfluidic particle dam which enables visual quantification of lead ions (Pb2+) the naked eye. GR-5 DNAzyme with extended termini can connect magnetic microparticles (MMPs) polystyrene (PMPs) DNA hybridization, forming "MMPs-GR-5-PMPs". When Pb2+ present, cleaved, resulting an increasing number free PMPs. To visually count PMPs, solution...

ABSTRACT Influenza virus hemagglutinin (HA) protein consists of two components, i.e., a globular head region and stem that are folded within six disulfide bonds, plus several N-linked glycans produce homotrimeric complex structure. While glycosylation sites on the variable among different strains subtypes, in mostly well conserved various influenza strains. Targeting highly HA regions has been proposed as useful strategy for designing universal vaccines. Since is constituted by an HA1...

Various COVID-19 vaccines are currently deployed, but their immunization varies and decays with time. Antibody level is a potent correlate to immune protection, its quantitation relies on intensive laboratory techniques. Here, we report decentralized, instrument-free microfluidic device that directly visualizes SARS-CoV-2 antibody levels. Magnetic microparticles (MMPs) polystyrene (PMPs) can bind antibodies simultaneously. In chip, this binding reduces the incidence of free PMPs escaping...

Recent experimental work has described an elegant pattern of branching in the development lung. Multiple forms have been identified, including side and tip bifurcation. A particularly interesting feature is phenomenon 'orthogonal rotation plane'. The lung must fill 3D space with essentially 2D branching. It accomplishes this by rotating plane 90° each generation. mechanisms underlying are not understood. In general, programmes that underlie hypothetically attributed to genetic 'subroutines'...

Cellular force regulates many types of cell mechanics and the associated physiological behaviors. Recent evidence suggested that motion with left-right (LR) bias may be origin LR asymmetry in tissue architecture. As actomyosin activity was found essential process, it predicts a type cellular coordinates development formation. However, due to lack appropriate platform, has not yet been found. Here we report nanowire magnetoscope reveals rotating force-torque-exerted by cells. Ferromagnetic...

Microcontact printing (μCP) is widely used to create patterns of biomolecules essential for studies cell mechanics, migration, and tissue engineering. However, different types μCPs may micropatterns with varied protein–substrate adhesion, which change behaviors pose uncertainty in result interpretation. Here, we characterize two μCP methods coating extracellular matrix (ECM) proteins (stamp-off covalent bond) demonstrate the first time important role adhesion determining behavior. We found...

Abstract Self-incompatibility (SI) in plants has evolved independently multiple times and S -RNase-based gametophytic self-incompatibility (GSI) is most common. The Rosaceae family possesses both self-recognition ( Prunus ) nonself-recognition Malus GSI systems, the latter widespread flowering plants. Gillenia trifoliata a species related to , providing utility for understanding SI evolution. sister taxon but unlike not undergone polyploidisation. In addition, common ancestor of close origin...

Abstract While genetic engineering has offered new strategies for regulating stem cell differentiation, the efficacy varies in cells with different phenotypes or lineage commitments, leading to inconsistent differentiation outcomes and uncertainty regenerative medicine. To address this issue, we employ a Y-shaped DNA (Y-DNA) as nanomaterial phenotype-specifically regulate of human mesenchymal (hMSCs). Y-DNA is composed three strands complementary sequences roles. The designed present study...

This paper presents a built-in self-test (BIST) design for fault detection and diagnosis of static-RAM (SRAM)-based field-programmable gate arrays (FPGAs). The proposed FPGA BIST structure can test both the interconnect resources [wire channels programmable switches (PSs)] lookup tables (LUTs) in configurable logic blocks (CLBs). pattern generator output response analyzer are configured by existing CLBs FPGAs; thus, no extra area overhead is needed structure. target detection/diagnosis...

When a sessile droplet evaporates, coffee-ring effect drives the suspended particulate matters to edge, eventually forming ring-shaped deposition. Because it causes non-uniform distribution of solid contents, which is undesired in many applications, attempts have been made eliminate effect. Recent reports indicated that can be suppressed by mixture spherical and non-spherical particles with enhanced particle-particle interaction at air-water interface. However, model comprehend...

Point-of-care devices offering quantitative results with simple steps would allow great useability for untrained end-users. Here, we report a ready-to-use chemosensor integrating automatic sample metering, on-chip reaction, gravitational–magnetic separation, and distance-based readout visual quantification of multiple heavy metal ions. Deoxyribozymes (DNAzymes), probe-modified magnetic microparticles (MMPs), polystyrene (PMPs) are preloaded into microfluidic chip freeze-dried. After the...

Abstract Cell chirality is observed with diverse forms and coordinates various left‐right (LR) asymmetry in tissue morphogenesis. To give rise to such diversity, cell may be coupled differentiation. Here, using micropatterned human mesenchymal stem cells (hMSCs), an early committed clockwise (CW) that can itself upregulate the adipogenic differentiation reported. hMSC enables a positively tilted chiral orientation on stripes. When cultured as single circular micropatterns, anticlockwise...

This paper presents the design method of very low-frequency current-mode low-pass filters for biomedical applications. The double-output operational transconductance amplifiers and grounded capacitors (DO-OTA-Cs) are used to realize proposed filters. theory focuses mainly on establishing a relationship between cascaded DO-OTA-Cs multiple-loop feedback connections, which make formulas. For implementation large time constants filters, both linearized DO-OTAs with reduced impedance scaler...