A5028864969- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- Chromatin Remodeling and Cancer
- Virus-based gene therapy research
- Cancer Mechanisms and Therapy
- Microtubule and mitosis dynamics
- Acute Myeloid Leukemia Research
- RNA and protein synthesis mechanisms
- Hematopoietic Stem Cell Transplantation
- Cutaneous lymphoproliferative disorders research
- Genomic variations and chromosomal abnormalities
- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- Plant Virus Research Studies
- Melanoma and MAPK Pathways
- Immune Cell Function and Interaction
- Epigenetics and DNA Methylation
- Chronic Lymphocytic Leukemia Research
- Mitochondrial Function and Pathology
- Chromosomal and Genetic Variations
University of Rhode Island
2017-2024
Stanford University
2022-2024
Dana-Farber Cancer Institute
2020-2023
Harvard University
2020-2023
CD123, a subunit of the interleukin-3 receptor, is expressed on ∼80% acute myeloid leukemias (AMLs). Tagraxofusp (TAG), recombinant fused to truncated diphtheria toxin payload, first-in-class drug targeting CD123 approved for treatment blastic plasmacytoid dendritic cell neoplasm. We previously found that AMLs with acquired resistance TAG were re-sensitized by DNA hypomethylating agent azacitidine (AZA) and TAG-exposed cells became more dependent antiapoptotic molecule BCL-2. Here, we report...
// David A. Vierra 1 , Jada L. Garzon Meghan Rego 2 Morganne M. Adroved Maurizio Mauro 3 and Niall G. Howlett Department of Cell Molecular Biology, University Rhode Island, Kingston, U.S.A Addgene, Cambridge, Massachusetts, Obstetrics & Gynecology Women's Health, Albert Einstein College Medicine, New York, Correspondence to: Howlett, email: nhowlett@uri.edu Keywords: fanconi anemia, FANCD2, FANCI, monoubiquitination, histone methylation Received: January 02, 2017 Accepted: June 10,...
Fanconi anemia (FA) is an inherited disease characterized by bone marrow failure and increased cancer risk. FA caused mutation of any 1 22 genes, the proteins function cooperatively to repair DNA interstrand cross-links (ICLs). A central step in activation pathway monoubiquitination FANCD2 FANCI proteins, which occurs within chromatin. How are anchored chromatin remains unknown. In this study, we identify characterize a histone-binding domain (HBD) embedded methyl-lysine-binding (MBD)...
Fanconi anemia (FA) is a rare genetic disease characterized by increased risk for bone marrow failure and cancer. The FA proteins function together to repair damaged DNA. A central step in the activation of pathway monoubiquitination FANCD2 FANCI proteins, which occurs upon exposure DNA-damaging agents during S phase cell cycle. regulatory mechanisms governing S-phase monoubiquitination, particular, are poorly understood. In this study, we have identified cyclin-dependent kinase (CDK)...
Abstract SWI/SNF has been shown to have important functions in hypoxia-mediated gene expression through roles of its catalytic and core subunits. Since exists as three distinct assemblies, usage complex specific subunits the can be expected vary within a given cell under changing environmental conditions. It remains an open question compositional makeup individual complexes viability hypoxic environment. In our current study, we find that hypoxia regulates levels unique define each complex....
Recombinant adeno-associated viral vectors (rAAV) hold an intrinsic ability to stimulate homologous recombination (AAV-HR) and are the most used in clinical settings for
Abstract Fanconi anemia (FA) is a rare genetic disease characterized by increased risk for bone marrow failure and cancer. The FA proteins function together to repair damaged DNA. A central step in the activation of pathway monoubiquitination FANCD2 FANCI under conditions cellular stress during S-phase cell cycle. regulatory mechanisms governing monoubiquitination, particular, are poorly understood. In this study, we have identified CDK phospho-site (S592) proximal site monoubiquitination....
Abstract ARID1A, a subunit of SWI/SNF, has been shown to play major role in recruitment the chromatin remodeler enhancers for transcriptional regulation. Mutations inARID1A have found various cancers, many which form solid tumors. Recent studies revealed vulnerabilities cells lacking ARID1A , specifically ARID1B, an ortholog and mutually exclusive subunit, 2D cell culture. However, identification within SWI/SNF loss multicellular tumor spheroid, that mimic vivo condition tumors, not...