A5024941227- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Fibroblast Growth Factor Research
- Bacterial Identification and Susceptibility Testing
- Acute Lymphoblastic Leukemia research
- Diphtheria, Corynebacterium, and Tetanus
- Antimicrobial Resistance in Staphylococcus
- CAR-T cell therapy research
- Mycobacterium research and diagnosis
- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- Clostridium difficile and Clostridium perfringens research
- Microbial infections and disease research
- Sarcoma Diagnosis and Treatment
- Hematopoietic Stem Cell Transplantation
- Cytokine Signaling Pathways and Interactions
- Streptococcal Infections and Treatments
- Escherichia coli research studies
- Cutaneous lymphoproliferative disorders research
- Vector-borne infectious diseases
- Infections and bacterial resistance
- Identification and Quantification in Food
- Veterinary medicine and infectious diseases
- Parasitic Infections and Diagnostics
Dana-Farber Cancer Institute
2021-2024
Harvard University
2023
University Pathologists
1995-2000
University of Utah
1996-2000
University of Washington
1992-1994
Harborview Medical Center
1992-1994
The challenge of eradicating leukemia in patients with acute myelogenous (AML) after initial cytoreduction has motivated modern efforts to combine synergistic active modalities including immunotherapy. Recently, the ETCTN/CTEP 10026 study tested combination DNA methyltransferase inhibitor decitabine together immune checkpoint ipilimumab for AML/myelodysplastic syndrome (MDS) either allogeneic hematopoietic stem cell transplantation (HSCT) or HSCT-naïve setting. Integrative...
"Mycobacterium genavense" is a proposed new species recently reported to cause disseminated infections in 18 patients with AIDS Europe. We have recovered "M. as slowly growing fastidious mycobacteria blood cultures of seven AIDS. In the original studies genavense," organism grew only BACTEC 13A vials. The Seattle, Washington, isolates also failed grow when subcultured from vials routine solid media, but dysgonic colonies were produced on Middlebrook 7H11 agar supplemented mycobactin J....
Two articles in this week’s issue focus on the use of ipilimumab and decitabine for patients with myelodysplasia (MDS) acute myeloid leukemia (AML) before after hematopoietic stem cell transplantation (HSCT) high-risk disease. In first article, Garcia et al report results a phase 1 trial combination 54 patients, demonstrating overall response rate 52% who are HSCT-naïve 20% post-HSCT; responses usually short-lived. second Penter colleagues characterize gene expression to therapy conclude...
CD123, a subunit of the interleukin-3 receptor, is expressed on ∼80% acute myeloid leukemias (AMLs). Tagraxofusp (TAG), recombinant fused to truncated diphtheria toxin payload, first-in-class drug targeting CD123 approved for treatment blastic plasmacytoid dendritic cell neoplasm. We previously found that AMLs with acquired resistance TAG were re-sensitized by DNA hypomethylating agent azacitidine (AZA) and TAG-exposed cells became more dependent antiapoptotic molecule BCL-2. Here, we report...
During a 14-month period, unique strain of Corynebacterium striatum that produces diffusible brown pigment was isolated from purulent sputa nine patients and nonrespiratory sites two additional patients. Seven nonpigmented clinical isolates the same period three reference strains C. were compared with isolates. Most had multiple sputum cultures no coryneforms before emerged, suggesting organism hospital acquired. DNA restriction fragment patterns Southern hybridization att site probe...
A rapid polymerase chain reaction (PCR) method for the direct detection of staphylococcal mecA gene from BACTEC blood culture bottles (Becton Dickinson, Sparks, MD) was developed. Published primer sequences and sample preparation using Achromopeptidase cell lysis were adapted to use Idaho Technology Air Thermocycler 1605 (Idaho Technologies, Falls, ID). The validated with 80 strains coagulase-positive coagulase-negative geographically diverse methicillin-resistant susceptible isolates...
An outbreak of methicillin-resistant Staphylococcus aureus infections at the University Utah Health Sciences Center occurred over a 7-month period. While isolates phenotypically appeared to be similar in gross morphology and have Vitek antibiotic susceptibility patterns, two additional methods strain characterization were evaluated enhance epidemiological investigation: pulsed-field gel electrophoresis gas chromatography with MIDI Sherlock system. uses qualitatively quantitatively analyze...
The Rapid CORYNE system for identification of aerobic, nonsporeforming, gram-positive rods was evaluated according to the manufacturer's instructions with 177 organisms. After inoculation a heavy suspension growth, strips containing 20 cupules were incubated 24 h, reagents added, and results 21 biochemical reactions recorded as numerical profiles. strains consisted pathogenic species genus Corynebacterium, primarily C. diphtheriae (n = 29), opportunistic Corynebacterium including jeikeium...
Antibiotic-resistant Corynebacterium striatum strains were isolated from 14 Harborview and one Veterans Administration Hospital patients in Seattle during the period 1987–90. These clindamycin-erythromycin resistant shown to hybridize with ermCd gene, which was cloned a diph-theriae plasmid encodes for rRNA methylase. Thirteen of these also hybridized tetM gene probes, tetracycline The could be transferred, by conjugation, while not transferable.
Attempts to identify coryneform isolates resembling Corynebacterium xerosis can lead clinical microbiologists identification schemes with conflicting descriptions which result in confusing C. striatum. For the present study we purchased all available American Type Culture Collection and National of Cultures reference cultures (n = 10) striatum 4) analyzed them as follows: (i) analysis biochemical reactions conventional tests Rapid CORYNE system, (ii) whole-cell fatty acid by using gas-liquid...
Shigellosis is hyperendemic in Utah. Most isolates are Shigella sonnei, making it difficult to identify epidemiologic clustering. To better define transmission, molecular markers and data were examined for 90 cases. Plasmid analysis pulsed-field gel electrophoresis (PFGE) of the S. sonnei identified 11 4 patterns, respectively. pattern I infections occurred 8 day care centers over a 6-month period, suggesting spread between centers. III was isolated from children at 3 additional IV...
Abstract Purpose: Patients with relapsed/refractory (R/R) acute myeloid leukemia (AML) have poor outcomes and require new therapies. In AML, autocrine production of hepatocyte growth factor (HGF) drives MET signaling that promotes myeloblast survival, making an attractive therapeutic target. inhibition exhibits activity in AML preclinical studies, but HGF upregulation by the FGFR pathway is a common mechanism resistance. Methods: We performed studies followed Phase I trial to investigate...
To adapt pulsed-field gel electrophoresis (PFGE) to a busy clinical laboratory, we evaluated the use of achromopeptidase (ACP) as sole agent for lysis variety gram-positive cocci. Growth in an appropriate broth media was adjusted 3.0 McFarland density before aliquotting washing and plug preparation. ACP incorporated into agarose plugs, or added buffer solution, lysed cells <1 h when suspended Tris-NaCl buffer. Two 30-min washes Tris-EDTA were sufficient remove cellular debris without...
The description of Corynebacterium striatum(Chester 1901) Eberson 1918ALin Bergey's Manual Systematic Bacteriology has many inconsistencies with the identification scheme from Centers for Disease Control and Prevention. We have studied four C. striatum reference strains available American Type Culture Collection National Cultures found that they fit Prevention this species. However, it appears wrong were deposited species, because none fits descriptions in original publications. This is...