A5064072738- Corneal surgery and disorders
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Ocular Surface and Contact Lens
- Ocular Infections and Treatments
- Intraocular Surgery and Lenses
- Ophthalmology and Visual Impairment Studies
- Ocular Diseases and Behçet’s Syndrome
- Bacterial biofilms and quorum sensing
- Herpesvirus Infections and Treatments
- Retinal and Macular Surgery
- Retinal and Optic Conditions
- Retinal Diseases and Treatments
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Ophthalmology and Eye Disorders
- Antibiotics Pharmacokinetics and Efficacy
- Autoimmune Bullous Skin Diseases
- Vascular Malformations Diagnosis and Treatment
- Optical Coherence Tomography Applications
- Retinal Imaging and Analysis
- Visual perception and processing mechanisms
- Angiogenesis and VEGF in Cancer
- Retinopathy of Prematurity Studies
- Ocular Oncology and Treatments
Royal Liverpool University Hospital
2016-2025
University of Liverpool
2016-2025
Aintree University Hospitals NHS Foundation Trust
2021-2025
Liverpool Hospital
2024
Royal Liverpool and Broadgreen University Hospital NHS Trust
1998-2023
St. Paul's Co-educational College
2003-2023
Vision Eye Institute
2021-2022
University College London
2021
Moorfields Eye Hospital
2021
NIHR Moorfields Biomedical Research Centre
2021
Abstract Aims/hypothesis Corneal confocal microscopy is a rapid non-invasive ophthalmic imaging technique that identifies peripheral and central neurodegenerative disease. Quantification of corneal sub-basal nerve plexus morphology, however, requires either time-consuming manual annotation or less-sensitive automated image analysis approach. We aimed to develop validate an artificial intelligence-based, deep learning algorithm for the quantification fibre properties relevant diagnosis...
To report early corneal biomechanical changes after cross-linking (CXL) in patients with keratoconus.Thirty-four eyes of 34 undergoing CXL for progressive keratoconus were included this prospective clinical study. Dynamic response (DCR) parameters obtained the Corvis ST (OCULUS Optikgeräte GmbH; Wetzlar, Germany) assessed at baseline (day CXL) and 1 month follow-up; conversely, tomography Pentacam GmbH) was 1, 3, 6 months CXL.At last follow-up visit (123.7 ± 69.6 days), all morphological...
To examine the efficacy and safety of corneal cross-linking (CXL) for stabilization progressive keratoconus.Observer-masked, randomized, controlled, parallel-group superiority trial.Sixty participants 10 to 16 years age with keratoconus, one eye each deemed study eye.The was randomized either CXL plus standard care or alone, spectacle contact lens correction as necessary vision.The primary outcome steep keratometry (K2) in a measure steepness cornea at 18 months. Secondary outcomes included...
Abstract A proportion of people with fibromyalgia demonstrate small fibre pathology (SFP). However, it is unclear how SFP directly relates to pain phenomenology. Thirty-three individuals FMS and ten healthy volunteers underwent assessment sensory phenotyping using corneal confocal microscopy, validated questionnaires quantitative testing (QST). Corneal nerve length was used stratify participants into [SFP+] without [SFP−]. detected in 50% the cohort. Current score QST parameters did not...
Background: This study aims to evaluate the repeatability of Pentacam HR, comparing two different measurement modes (50-cornea fine and 25-3D scan) in patients affected by keratoconus. Methods: Multicenter retrospective study, conducted at Eye Clinic ASST-Spedali Civili-University Brescia, Italy, St. Paul’s Unit, Royal Liverpool University Hospital, United Kingdom. A total 72 eyes from with keratoconus underwent six consecutive measurements, three using scan mode 50-Cornea mode. Measurements...
To compare outcomes after penetrating keratoplasty (PK) and deep anterior lamellar (DALK) for keratoconus in the United Kingdom.Patient outcome data were collected at time of transplantation 1, 2, 5 years surgery. Data analyzed by Kaplan-Meier survival curves, Cox regression, binary logistic regression to determine influence surgical procedure on graft visual outcome.The risk failure DALK was almost twice that PK (P = 0.02). Nineteen percent failures occurred first 30 postoperative days...
Purpose.: To investigate the relationship between susceptibility of bacteria to topical antimicrobials and clinical outcome in microbial keratitis. Methods.: Clinical data were collected from patients with keratitis whom a bacterium had been isolated during period 2003 2006. The minimum inhibitory concentration (MIC) was determined for isolates against 10 antimicrobials. determinants primary outcome, ratio healing time (closure epithelial defect) ulcer size (HT/UA), analyzed general linear...
Pseudomonas aeruginosa is a common opportunistic bacterial pathogen that causes variety of infections in humans. Populations P. are dominated by clones can be isolated from diverse clinical and environmental sources. To determine whether specific associated with corneal infection, we used portable genotyping microarray system to analyze set 63 isolates patients ulcers (keratitis). We then population analysis compare the keratitis wider collection various nonocular identified markers...
Purpose.: To determine the minimum inhibitory concentrations (MICs) of 12 antimicrobials in current ophthalmic use and 4 potentially new alternatives against isolates from bacterial keratitis. Methods.: Bacteria were collected cases keratitis six centers United Kingdom between 2003 2006. MICs measured by using susceptibility strips containing a concentration gradient penicillin, cefuroxime, ceftazidime, chloramphenicol, gentamicin, amikacin, vancomycin, teicoplanin, ciprofloxacin, ofloxacin,...
Background/aims Descemet membrane endothelial keratoplasty (DMEK) preparation is technically demanding and a limiting factor for uptake of this kind surgery. Supply methods that simplify the procedure surgeons are key to increasing uptake. This study compares two different shipping protocols DMEK. Methods An 8.5 mm DMEK graft was punched, marked loaded transportation in conditions: (A) endothelium trifolded inwards organ culture conditions (n=7) (B) rolled outwards hypothermic (n=7). Tissues...
To evaluate the accuracy of convolutional neural networks technique (CNN) in detecting keratoconus using colour-coded corneal maps obtained by a Scheimpflug camera.Multicentre retrospective study.We included images keratoconic and healthy volunteers' eyes provided three centres: Royal Liverpool University Hospital (Liverpool, UK), Sedaghat Eye Clinic (Mashhad, Iran) The New Zealand National Center (New Zealand). Corneal tomography scans were used to train test CNN models, which controls....
Abstract Aims/hypothesis We aimed to develop an artificial intelligence (AI)-based deep learning algorithm (DLA) applying attribution methods without image segmentation corneal confocal microscopy images and accurately classify peripheral neuropathy (or lack of). Methods The AI-based DLA utilised convolutional neural networks with data augmentation increase the algorithm’s generalisability. was trained using a high-end graphics processor for 300 epochs on 329 nerve tested 40 (1...
<h3>AIMS</h3> To determine the sensitivity and specificity of culture, immunohistochemistry (IHC), polymerase chain reaction (PCR), in situ hybridisation (ISH) for detecting herpes simplex virus (HSV-1) cornea patients undergoing penetrating keratoplasty. compare incidence HSV-1 with that varicella zoster (VZV), cytomegalovirus (CMV), Epstein-Barr (EBV). <h3>METHODS</h3> The corneas 110 patients, 52 a documented history keratitis (HSK) 58 non-herpetic corneal disease, were investigated using...
Patients undergoing penetrating keratoplasty for prior herpes simplex keratitis (group A) and corneal disease unrelated to B) were investigated assess whether the cornea is a site viral latency. All patients seropositive antibody. Virus was isolated from tear film postoperatively in one patient on cocultivation of another patient. Herpes DNA, however, detected corneas all group A half those B by means polymerase chain reaction primers three well separated regions genome. Three donor had no...
PURPOSE.Mutations in ZEB1 have been reported posterior polymorphous corneal dystrophy (PPCD3; MIM #609141) and Fuchs' endothelial (FECD6; #613270).Although PPCD keratoconus are clinically pathologically distinct, has associated with keratoconus, suggesting a common genetic basis.The purpose of our study was to perform mutational screening the gene patients affected or PPCD.METHODS.Sanger sequencing performed 70 unrelated 18 PPCD.Real-time quantitative PCR (RT-qPCR) on RNA from cultured...
Purpose.: To evaluate indocyanine green angiography (ICGA) and fluorescein (FA) in imaging quantifying corneal neovascularization (CNV). Methods.: Patients with CNV were studied using a standardized protocol of color digital photography, FA, ICGA. Images graded independently by two observers assessed for quality, phases fluorescence, leakage. Areas vasculature geometric properties analyzed quantified an automated program. Results.: Twenty-three patients good quality images included. Mean...