A5018568072- Cardiac electrophysiology and arrhythmias
- MicroRNA in disease regulation
- Angiogenesis and VEGF in Cancer
- Breast Cancer Treatment Studies
- RNA modifications and cancer
- Cell Adhesion Molecules Research
- Aortic aneurysm repair treatments
- Autophagy in Disease and Therapy
- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- Monoclonal and Polyclonal Antibodies Research
- Circular RNAs in diseases
- Ion channel regulation and function
- HER2/EGFR in Cancer Research
- Endoplasmic Reticulum Stress and Disease
- Coronary Interventions and Diagnostics
- Cardiac Fibrosis and Remodeling
- Acute Myocardial Infarction Research
- Platelet Disorders and Treatments
- Protease and Inhibitor Mechanisms
- Cancer survivorship and care
- Cardiac Arrhythmias and Treatments
- Peptidase Inhibition and Analysis
- Cardiovascular Effects of Exercise
- Receptor Mechanisms and Signaling
Shanghai Jiao Tong University
2008-2025
Jiangsu Cancer Hospital
2012-2024
Huazhong University of Science and Technology
2015-2024
Nanjing Medical University
2015-2024
Ruijin Hospital
2023
Shanghai Ninth People's Hospital
2023
Institute of Medical Biology
2021
Chinese Academy of Medical Sciences & Peking Union Medical College
2010-2021
Boehringer Ingelheim (Germany)
2021
Shandong Agricultural University
2019
Endoplasmic reticulum stress is an evolutionarily conserved cell response associated with numerous diseases, including cardiac hypertrophy and heart failure. The major endoplasmic signaling pathway causing involves sensor PERK (protein kinase-like kinase) eIF2α-ATF4-CHOP signaling. Here, we describe a non-canonical, AGGF1-mediated regulatory system for increased p-eIF2α ATF4 decreased sXBP1 CHOP. Specifically, see reduced AGGF1 level consistently induction of in mouse models human patients...
Abstract Aims Cardiac fibrosis is a major cause of heart failure (HF), and mediated by the differentiation cardiac fibroblasts into myofibroblasts. However, limited tools are available to block fibrosis. ADAMTS16 member ADAMTS superfamily extracellular protease enzymes involved in matrix (ECM) degradation remodelling. In this study, we aimed establish as key regulator Methods results Western blot qRT–PCR analyses demonstrated that was significantly up-regulated mice with transverse aortic...
The aim of this study was to identify the molecular mechanism for hyperglycaemia-induced metabolic memory in endothelial cells (ECs), and show its critical importance development cardiovascular dysfunction diabetes.
Background: Currently, exosomes that act as mediators of intercellular communication are being researched extensively. Our previous studies confirmed these contain microRNAs (miRNAs) could alter chemo-susceptibility, which is partly attributed to the successful transfer multidrug resistance (MDR)-specific miRNAs. We also β-elemene influence MDR-related miRNA expression and regulate target genes PTEN Pgp, may lead reversal chemoresistant breast cancer (BCA) cells. first report findings, we...
AGGF1 is an angiogenic factor with therapeutic potential to treat coronary artery disease (CAD) and myocardial infarction (MI). However, the underlying mechanism for AGGF1-mediated angiogenesis unknown. Here, we show first time that activates autophagy, a housekeeping catabolic cellular process, in endothelial cells (ECs), HL1, H9C2, vascular smooth muscle cells. Studies Atg5 small interfering RNA (siRNA) autophagy inhibitors bafilomycin A1 (Baf) chloroquine demonstrate required EC...
T helper 17 cells and interleukin-17A have been implicated in the progression of abdominal aortic aneurysm (AAA). Retinoic acid-related orphan receptor gamma thymus, master transcription factor cell differentiation, is selectively antagonized by digoxin. However, effect antagonizing retinoic thymus on AAA has not investigated.We used human sample analysis 2 different experimental models: (a) Angiotensin II (Ang II)-induced ApoE(-/-) male mice II/APOE model) (b) porcine pancreatic elastase...
Abstract In type 1 and 2 diabetes mellitus, increased cardiac fibrosis, stiffness associated diastolic dysfunction may be the earliest pathological phenomena in diabetic cardiomyopathy. Endothelial‐mesenchymal transition (EndMT) endothelia cells (ECs) is a critical cellular phenomenon that increases fibroblasts (CFs) fibrosis hearts. The purpose of this paper to explore molecular mechanism miR‐21 regulating EndMT perivascular vivo, hyperglycaemia up‐regulated mRNA level , aggravated collagen...
Abstract Thoracic aortic aneurysm (TAA) is a localized or diffuse dilatation of the thoracic aortas, and causes many sudden deaths each year worldwide. However, there no effective pharmacologic therapy. Here, we show that AGGF1 effectively blocks TAA-associated arterial inflammation remodeling in three different mouse models (mice with transverse constriction, Fbn1 C1041G/+ mice, β-aminopropionitrile-treated mice). expression reduced ascending aortas from human TAA patients. Aggf1 +/- mice...
Atrial fibrillation (AF) is the most common cardiac arrhythmia at clinic. Recent GWAS identified several variants associated with AF, but they account for <10% of heritability. Gene-gene interaction assumed to a significant portion missing Among loci only three were replicated in Chinese Han population, including SNP rs2106261 (G/A substitution) ZFHX3, rs2200733 (C/T near PITX2c, and rs3807989 (A/G CAV1. Thus, we analyzed among these AF loci. We demonstrated between independent populations...
MicroRNA (miR)-146a-5p functions as a tumor suppressor in various types of cancer. However, the role miR-146a-5p development triple-negative breast cancer (TNBC) is unclear. The present study aimed to investigate TNBC. expression level TNBC tissues and cell lines was initially detected using reverse transcription-quantitative polymerase chain reaction. To predict target gene miR-146a-5p, TargetScan software used dual luciferase assay performed verify prediction. Furthermore, order explore...
Background Peripheral arterial disease (PAD) is a common accounting for about 12% of the adult population, and causes significant morbidity mortality. Therapeutic angiogenesis using angiogenic factors has been considered to be potential treatment option PAD patients. In this study, we assessed new factor AGGF1 therapeutic in critical limb ischemia model mice PAD. Methods Results We generated unilateral hindlimb by ligation right iliac artery femoral artery. Ischemic with intrasmuscular...
Aggf1 is the first gene identified for Klippel-Trenaunay syndrome (KTS), and encodes an angiogenic factor. However, in vivo roles of are incompletely defined. Here we demonstrate that essential both physiological angiogenesis pathological tumour vivo. Two lines knockout (KO) mice showed a particularly severe phenotype as no homozygous embryos were observed heterozygous also embryonic lethality (haploinsufficient lethality) only Vegfa Dll4. Aggf1+/− KO caused defective yolk sacs embryos....
Cardiac fibrosis is the primary cause of deteriorated cardiac function in various cardiovascular diseases. Numerous studies have demonstrated that microRNAs (miRNAs) are critical regulators myocardial fibrosis. Specifically, many reported miR-150 downregulated diseases, such as acute infarction (AMI), hypertrophy and However, exact role these pathological processes remains unknown.We used transverse aortic constriction (TAC) mouse model to study induced by pressure overload. After TAC...
The endothelial-to-mesenchymal transition (EMT) of glomerular vascular endothelial cells is considered to be pivotal in diabetic nephropathy (DN). risk DN can decreased by losartan, but the potential molecular mechanism(s) are not fully understood. Extensive data show that EMT occurs proximal tubular resulting an phenotype switch (fibrotic matrix accumulation), consequently enhancing development renal interstitial fibrosis. Here, we found losartan significantly ameliorated DN-induced...
Abstract Hepatocellular carcinoma (HCC) is one of the most common malignant cancers. To elucidate new regulatory mechanisms for heptocarcinogenesis, we investigated regulation p21, a cyclin-dependent kinase (CDK) inhibitor encoded by CDKN1A , in HCC. The expression level p21 decreased with progression Luciferase assays luciferase-p21-3′ UTR reporter and its serial deletions identified 15-bp repressor element at 3′-UTR which contains binding site miR-95-3p . Mutation eliminated effect on...
Background: In recent years, tRFs(transfer RNA-Derived Fragments) and transfer RNADerived Stress-induced RNAs (or tRNA halves) have been shown to vital roles in cancer biology. We aimed reveal the expression profile of tRNA-derived fragments breast tissues study, explore their potential as biomarkers cancer. Methods: characterized from 6 paired clinical adjacent normal samples. Then we selected significantly expressed tRNAderived screened genes for validation by using Quantitative Real-time...
Hyperglycemia-triggered vascular abnormalities are the most serious complications of diabetes mellitus (DM). The major cause dysfunction in DM is endothelial injury and associated with reduced number progenitor cells (EPCs). A challenge to identify key regulators EPCs restore DM-associated dysfunction. We show that from heterozygous knockout Aggf1+/− mice presented impairment proliferation, migration, angiogenesis, transendothelial migration as hyperglycemic fed a high-fat diet (HFD) or...