A5058045699- Plant Virus Research Studies
- Plant and Fungal Interactions Research
- Insect-Plant Interactions and Control
- Animal Virus Infections Studies
- Agricultural Science and Fertilization
- Crop Yield and Soil Fertility
- Rice Cultivation and Yield Improvement
- Plant Disease Resistance and Genetics
- Analytical Methods in Pharmaceuticals
- Agricultural Economics and Practices
- Plant Pathogenic Bacteria Studies
- Natural Antidiabetic Agents Studies
- Insect symbiosis and bacterial influences
- Plant Parasitism and Resistance
- Soil Carbon and Nitrogen Dynamics
- Bioenergy crop production and management
- Peanut Plant Research Studies
- Ethnobotanical and Medicinal Plants Studies
- Drug Solubulity and Delivery Systems
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Agronomic Practices and Intercropping Systems
- Viral gastroenteritis research and epidemiology
- Phytoplasmas and Hemiptera pathogens
- Nitrogen and Sulfur Effects on Brassica
Agricultural Research Service
2016-2025
University of Nebraska–Lincoln
2016-2025
United States Department of Agriculture
2015-2025
International Plant Nutrition Institute
2014-2020
K+S (Germany)
2020
Andhra University
2006-2017
International Institute of Tropical Agriculture
2016-2017
Dr. Reddy's Laboratories (India)
2017
National Alliance on Mental Illness
2015
University of Florida
2001-2012
Huanglongbing (HLB) is one of the most devastating diseases citrus worldwide, and caused by a phloem-limited fastidious prokaryotic alpha-proteobacterium that yet to be cultured. In this study, combination traditional polymerase chain reaction (PCR) real-time PCR targeting putative DNA 16S rDNA sequence 'Candidatus Liberibacter asiaticus,' respectively, were used examine distribution movement HLB pathogen in infected tree. We found 'Ca. asiaticus' was distributed bark tissue, leaf midrib,...
Citrus tristeza virus (CTV) is a phloem-limited whose natural host range restricted to citrus and related species. Although the has killed millions of trees, almost destroying whole industries, continually limits production in many growing areas, most isolates are mild or symptomless their range. There little understanding how causes severe disease some none others. Movement distribution CTV differs considerably from that well-studied viruses herbaceous plants where movement occurs largely...
Reduction of excess nutrient application and balanced fertilizer use are the key mitigation options in agriculture. We evaluated Nutrient Expert (NE) tool-based site-specific management (SSNM) rice wheat crops by establishing 1594 side-by-side comparison trials with farmers' fertilization practices (FFP) across Indo-Gangetic Plains (IGP) India. found that NE-based can lower global warming potential (GWP) about 2.5% rice, between 12 20% over FFP. More than 80% participating farmers increased...
Citrus tristeza virus (CTV) populations in citrus trees are unusually complex mixtures of viral genotypes and defective RNAs developed during the long-term vegetative propagation by additional mixing aphid transmission. The replication process allows maintenance minor amounts disparate these populations. CTV is a member Closteroviridae possessing positive-stranded RNA genome ≈20 kilobases that expresses replicase-associated genes as an ≈400-kDa polyprotein remaining 10 3′ through subgenomic...
The long flexuous virions of the Closteroviridae have a unique bipolar architecture incorporating two coat proteins, with most helical nucleocapsid encapsidated by major protein (CP) and small portion one end minor (CPm). It is not known whether CPm encapsidates genomic RNA and, if so, which what effects transition between proteins. Two other virus-encoded an HSP70 homolog (HSP70h) ≈61-kDa protein, are required to augment virion assembly. In this work, we examine in vivo encapsidation Citrus...
ABSTRACT The first Citrus tristeza virus (CTV) genomes completely sequenced (19.3-kb positive-sense RNA), from four biologically distinct isolates, are unexpectedly divergent in nucleotide sequence (up to 60% divergence). Understanding of whether these large differences resulted recent evolution is important for the design disease management strategies, particularly use genetically engineered mild (essentially symptomless)-strain cross protection and RNA-mediated transgenic resistance....
Triticum mosaic virus (TriMV), the type member of newly proposed Poacevirus genus, and Wheat streak (WSMV), Tritimovirus genus family Potyviridae, infect wheat naturally in Great Plains are transmitted by curl mites. In this study, we examined ability these viruses to selected cereal hosts, found several differential hosts between TriMV WSMV. Additionally, interaction WSMV three cultivars at two temperature regimens (19 20 26 degrees C), quantified concentration single double infections...
Viruses have evolved as combinations of genes whose products interact with cellular components to produce progeny virus throughout the plants. Some viral genes, particularly those that are involved in replication and assembly, tend be relatively conserved, whereas other for interactions specific host movement counter host–defense systems less conserved. Closteroviridae encode 1–5 nonconserved ORFs. Citrus tristeza (CTV), a Closterovirus , possesses p33, p18, p13 expendable systemic infection...
ABSTRACT Eriophyid mite-transmitted, multipartite, negative-sense RNA plant viruses with membrane-bound spherical virions are classified in the genus Emaravirus . We report here that eriophyid mite-transmitted Wheat mosaic virus (WMoV), an , contains eight genomic segments, most a known virus. Remarkably, two 3 consensus sequences, encoding nucleocapsid protein, were found 12.5% sequence divergence, while no heterogeneity was observed sequences of additional segments. The RNA-dependent...
Abstract Background Wheat streak mosaic virus (WSMV) and Triticum (TriMV) are components of the wheat disease complex in Great Plains region U.S.A. elsewhere. Co-infection with WSMV TriMV causes synergistic interaction more severe symptoms compared to single infections. Plants equipped multiple antiviral mechanisms, which regulation microRNAs (miRNAs) is a potentially effective constituent. In this investigation, we have analyzed total relative expression miRNA transcriptome two cultivars,...
Plant diseases significantly impact food security and safety. It was estimated that production needs to increase by 50% feed the projected 9.3 billion people 2050. Yet, plant pathogens pests are documented cause up 40% yield losses in major crops, including maize, rice, wheat, resulting annual worldwide economic of approximately US$220 billion. Yield due be 21.5% (10.1 28.1%) 30.3% (24.6 40.9%) 22.6% (19.5 41.4%) maize. In March 2023, The American Phytopathological Society (APS) conducted a...
The complete genome sequence of Triticum mosaic virus (TriMV), a member in the family Potyviridae, has been determined to be 10,266 nucleotides (nt) excluding 3' polyadenylated tail. encodes large polyprotein 3,112 amino acids with "hall-mark proteins" potyviruses, including small overlapping gene, PIPO, P3 cistron. TriMV an unusually long 5' nontranslated region 739 nt 12 translation initiation codons and three open reading frames, which resemble those internal ribosome entry site...
Citrus tristeza virus (CTV), a member of the Closteroviridae, has 19.3-kb positive-stranded RNA genome that is organized into 12 open reading frames (ORFs) with 10 3' genes expressed via nested set nine or ten 3'-coterminal subgenomic mRNAs (sgRNAs). Relatively large amounts negative-stranded RNAs complementary to both genomic and sgRNAs accumulate in infected cells. As characteristic viruses, wild-type CTV produced more positive than negative strands, plus-to-minus ratios estimated at 20:1...
ABSTRACT Systemic invasion of plants by viruses is thought to involve two processes: cell-to-cell movement between adjacent cells and long-distance that allows the virus rapidly move through sieve elements unload at growing parts plant. There a continuum proportions these processes determines degrees systemic infection different viruses. We examined distribution Citrus tristeza (CTV) in citrus species with range susceptibilities. By using “pure” culture CTV from cDNA clone green fluorescent...
Citrus tristeza virus (CTV) (genus Closterovirus, family Closteroviridae) causes some of the more important viral diseases citrus worldwide. The ability to map disease-inducing determinants CTV is needed develop better diagnostic and disease control procedures. A distinctive phenotype isolates induce seedling yellows (SY) in sour orange, lemon grapefruit seedlings. In Florida, decline isolate CTV, T36, induces SY, whereas a widely distributed mild isolate, T30, does not. To delimit sequences...
Triticum mosaic virus (TriMV) and Wheat streak (WSMV) infect winter wheat (Triticum aestivum) in the Great Plains region of United States. The two viruses are transmitted by curl mites (Aceria tosichella), which also transmit High virus. In a field study conducted 2011 2012, cultivars Millennium (WSMV-susceptible) Mace (WSMV-resistant) were mechanically inoculated with TriMV, WSMV, TriMV+WSMV, or sterile water at two-leaf growth stage. Chlorophyll meter (soil plant analysis development...