A5058132071- Lipid Membrane Structure and Behavior
- Pancreatitis Pathology and Treatment
- Cholinesterase and Neurodegenerative Diseases
- Erythrocyte Function and Pathophysiology
- Protein Tyrosine Phosphatases
- Cancer, Hypoxia, and Metabolism
- Metabolomics and Mass Spectrometry Studies
- Galectins and Cancer Biology
- Hypothalamic control of reproductive hormones
- Pesticide Exposure and Toxicity
- Adipose Tissue and Metabolism
- Cancer, Lipids, and Metabolism
- Sphingolipid Metabolism and Signaling
- Computational Drug Discovery Methods
- Enzyme function and inhibition
- Pancreatic function and diabetes
- Fatty Acid Research and Health
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Protein Interaction Studies and Fluorescence Analysis
- Lipid metabolism and biosynthesis
- Immune cells in cancer
- Receptor Mechanisms and Signaling
- Peptidase Inhibition and Analysis
- Cholesterol and Lipid Metabolism
- Redox biology and oxidative stress
Instituto de Investigación Biomédica de Salamanca
2015-2024
Universidad de Salamanca
2010-2023
University of Iowa
1992-1994
Universidad de Alcalá
1992
Using a cell line stably transfected with the rat follitropin (FSH) receptor cDNA we demonstrate that FSH becomes phosphorylated when cells are exposed to FSH. Since binding of its results in an increase cAMP and inositol phosphate accumulation, examined potential involvement protein kinase A C mediating phosphorylation. Stimulation does not appear be necessary because hFSH-induced phosphorylation was minimally impaired overexpresses phosphodiesterase. Moreover, stimulation pathway other...
It is well established that the LH/CG receptor expressed in gonadal cells an 85- to 92-kilodalton (kDa) glycoprotein. Additionally, however, a number of reports have noted existence other putative species, but few attempts been made characterize these variant species. A cell line [293L(wt1)] had previously isolated which expresses large numbers high affinity surface receptors. Visualization species and rat luteal using ligand blots revealed 90-kDa receptors, respectively, while immunoblots...
An agonist-induced change in the functional properties of a constant number receptors seems to be ubiquitous phenomenon involved regulation cell surface receptors. Although mechanisms responsible for this (called uncoupling or desensitization) have been studied detail using beta 2-adrenergic it is unclear if models derived from these studies are applicable other members family G protein-coupled Since has shown previously that truncation C-terminal cytoplasmic tail receptor results delay...
Javier De Castroa, Angel Hernández-Hernándezb, Marina C. Rodríguezc, José L. Sardinab, Marcial Llanillob & Jesús Sánchez-Yagüeb*a Radiology Service, Santísima Trinidad Foundation Hospital, Salamanca, Spainb Department of Biochemistry and Molecular Biology, University Spainc Pneumology Service (Neumosalud), Spain
Much of the definitive work on G-protein-coupled receptor phosphorylation and its impact function has been performed with catecholamine receptors. Evidence for is lacking, however, receptors that bind larger ligands, such as LH/CG. Using immunoprecipitation techniques a clonal cell line stably transfected LH/CG receptor, we show here first time exposure cells to hCG induces cognate receptor. The hCG-induced increase in requires activation because it cannot be elicited antagonist mediated at...
To analyze the fatty acid profiles of erythrocyte total lipids from patients with advanced squamous cell lung carcinoma (SCC), adenocarcinoma (ADC), and small cancer (SCLC) benign diseases (chronic obstructive pulmonary disease [COPD] asthma) to reveal acids that could be used as biomarkers.Thirty, 20, 15, 17, 19 SCC, ADC, SCLC, COPD, asthma, respectively, 55 healthy participants were enrolled in our study. Fatty investigated using gas chromatography/mass spectrometry followed by receiver...
New biomarkers for lung cancer would be valuable. Our aim was to analyze the fatty acid profiles of main phospholipid species in erythrocytes from patients with advanced squamous cell carcinoma (SCC), adenocarcinoma (ADC), and small (SCLC) benign diseases (chronic obstructive pulmonary disease (COPD) asthma) determine acids that could use as markers. Twenty-eight, 18, 14, 16, 15 with, respectively, SCC, ADC, SCLC, asthma, COPD 50 healthy subjects were enrolled study. Fatty investigated using...
Acute myeloid leukaemia (AML) is a highly heterogeneous disease, however the therapeutic approaches have hardly changed in last decades. Metabolism rewiring and enhanced production of reactive oxygen species (ROS) are hallmarks cancer. A deeper understanding these features could be instrumental for development specific AML-subtypes treatments. NADPH oxidases (NOX), only cellular system specialised ROS production, also involved leukemic metabolism control. NOX2 shows variable expression AML...
The regulation of hematopoietic stem cells (HSCs) depends on the integration multiple signals received from bone marrow niche. We show relevance protein tyrosine phosphatase PTPN13 and β-catenin as intracellular signaling molecules to control HSCs adhesiveness, cell cycling, quiescence. Lethally irradiated mice transplanted with Lin– in which or had been silenced showed a significant increase long-term (LT) short-term (ST) HSCs. A decrease cycling was also found, together an decreased...
In most experiments done in cell-free systems, the LH/CG-induced desensitization of ovarian LH/CG-responsive adenylyl cyclase has been reported to be dependent on GTP. Little is known, however, about molecular basis this phenomenon or FSH-induced FSH-responsive cyclase. We report here that, contrary previous findings, ATP required for LH/CG- and human kidney cells stably transfected with complementary DNAs rat LH/CG FSH receptor. This requirement does not seem peculiar because under our...
The formation of free radicals and lipid peroxidation products is linked both to carcinogenesis tumor behavior. Blood samples from 50 patients with advanced (Stages III–IV) non-small cell lung cancer (NSCLC), healthy volunteers were used for plasma β-thromboglobulin (β-TG) measurements, red blood (RBC) platelet analyses, fluorescence determinations. Samples 15 randomly selected controls also analysis the expression oxidized proteins. We observed: (a) higher levels β-TG in patients, (b)...