A5017193518- Microtubule and mitosis dynamics
- Genetic and Kidney Cyst Diseases
- Fungal and yeast genetics research
- Genetic Neurodegenerative Diseases
- Micro and Nano Robotics
- Photosynthetic Processes and Mechanisms
- Nuclear Structure and Function
- Cellular Mechanics and Interactions
Delft University of Technology
2019-2025
Centrioles are microtubule-based organelles required for the formation of centrosomes and cilia. Centriolar microtubules, unlike their cytosolic counterparts, stable grow very slowly, but underlying mechanisms poorly understood. Here, we reconstituted in vitro interplay between proteins that cap distal centriole ends control elongation: CP110, CEP97, CPAP/SAS-4. We found whereas CEP97 does not bind to microtubules directly, CP110 autonomously binds microtubule plus ends, blocks growth,...
Abstract Growing microtubule ends organize end-tracking proteins into comets of mixed composition. Here using a reconstituted fission yeast system consisting end-binding protein Mal3, kinesin Tea2 and cargo Tip1, we found that these can be driven liquid-phase droplets both in solution at under crowding conditions. In the absence agents, cryo-electron tomography revealed motor-dependent consist disordered networks where multivalent interactions may facilitate non-stoichiometric accumulation...
Abstract Centrioles are microtubule-based organelles required for the formation of centrosomes and cilia. Centriolar microtubules, unlike their cytosolic counterparts, grow very slowly stable. The complex centriolar proteins CP110 CEP97 forms a cap that stabilizes distal centriole end prevents its over-elongation. Here, we used in vitro reconstitution assays to show whereas does not interact with microtubules directly, specifically binds microtubule plus ends, potently blocks growth induces...
Abstract Growing microtubule ends provide platforms for the accumulation of plus-end tracking proteins that organize into comets mixed protein composition. Using a reconstituted fission yeast system consisting end-binding Mal3, kinesin Tea2 and cargo Tip1, we found these can be driven liquid phase droplets both in solution at under crowding conditions. In absence agents, cryo-electron tomography revealed motor-dependent consist disordered networks where multivalent interactions appear to...