A5090007785- Molecular Sensors and Ion Detection
- Advanced biosensing and bioanalysis techniques
- Electron Spin Resonance Studies
- Mass Spectrometry Techniques and Applications
- Redox biology and oxidative stress
- Carbohydrate Chemistry and Synthesis
- Advanced Fluorescence Microscopy Techniques
- Sulfur Compounds in Biology
- ATP Synthase and ATPases Research
- Enzyme Structure and Function
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Eicosanoids and Hypertension Pharmacology
- Yeasts and Rust Fungi Studies
- Click Chemistry and Applications
- Enzyme Production and Characterization
- Luminescence and Fluorescent Materials
- Biofuel production and bioconversion
- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Viral gastroenteritis research and epidemiology
- Chemical Reactions and Isotopes
- Nicotinic Acetylcholine Receptors Study
University of Virginia
2018-2023
Oak Ridge National Laboratory
2021
University of California, Riverside
2015-2017
Redox signaling and homeostasis are important for all forms of life on Earth. There has been great interest in monitoring redox dynamics living cells organisms as a mean to better understand biology physiological pathological conditions. Herein we report our recent results the development genetically encoded redox-sensitive red fluorescent protein (rxRFP). We first identified circularly permuted RFP (cpRFP) scaffold, which maintained its autocatalytic fluorescence, from Ca2+ sensor, R-GECO1....
Hydrogen sulfide (H2S) is an important gasotransmitter. Although a large number of fluorescent probes for cellular H2S have been reported, only few can detect in mitochondria, organelle connecting with mitochondrial function and metabolic pathways. We hereby describe novel near-infrared probe, nimazide, by introducing sulfonyl azide to the core structure QSY-21 dark quencher. Nimazide responded quickly H2S, resulting robust fluorescence turn-off changes. This conversion displayed high...
Boronic acid-containing fluorescent molecules have been widely used to sense hydrogen peroxide and peroxynitrite, which are important reactive oxygen nitrogen species in biological systems. However, it has challenging gain specificity. Our previous studies developed genetically encoded, green peroxynitrite biosensors by incorporating a boronic noncanonical amino acid (ncAA),
There is great interest in developing boronolectins that are synthetic lectin mimics containing a boronic acid functional group for reversible recognition of diol-containing molecules, such as glycans and ribonucleotides. However, it remains significant challenge to gain specificity. Here, we present genetically encoded boronolectin which hybrid protein consisting noncanonical amino (ncAA)
There is great interest in developing boronolectins, which are synthetic lectin mimics containing a boronic acid functional group for reversible recognition of diol-containing molecules, such as glycans and ribonucleotides. However, it remains significant challenge to gain specificity. Here, we present genetically encoded boronolectin, hybrid protein consisting noncanonical amino (ncAA) p-boronophenylalanine (pBoF), natural-lectin-derived peptide sequences, circularly permuted red...
ABSTRACT Boronic acid-containing fluorescent molecules have been widely used to sense hydrogen peroxide and peroxynitrite, which are important reactive oxygen nitrogen species in biological systems. However, it has challenging gain specificity. Our previous studies developed genetically encoded, green peroxynitrite biosensors by incorporating a boronic noncanonical amino acid (ncAA), p -boronophenylalanine ( BoF), into the chromophore of circularly permuted proteins (cpGFPs). In this work,...