A5063673202- Sleep and Wakefulness Research
- Neuroscience and Neuropharmacology Research
- Circadian rhythm and melatonin
- Sleep and related disorders
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Alzheimer's disease research and treatments
- Neural dynamics and brain function
- EEG and Brain-Computer Interfaces
- Cannabis and Cannabinoid Research
- Adipose Tissue and Metabolism
- Photoreceptor and optogenetics research
- Neuroscience of respiration and sleep
- Regulation of Appetite and Obesity
- Olfactory and Sensory Function Studies
- Neurogenetic and Muscular Disorders Research
- RNA Interference and Gene Delivery
- Child Nutrition and Feeding Issues
- Neurogenesis and neuroplasticity mechanisms
- Developmental and Educational Neuropsychology
- Restless Legs Syndrome Research
- Retinal Imaging and Analysis
- Obstructive Sleep Apnea Research
- Protein Kinase Regulation and GTPase Signaling
Universidad Autónoma de Madrid
2013-2024
Cornell University
2001-2022
Instituto de Neurociencias
2006-2022
MIND Research Institute
2013-2022
Weill Cornell Medicine
2022
Manchester Academic Health Science Centre
2020
University of Manchester
2020
Hospital La Paz Institute for Health Research
2010-2016
Hospital Universitario La Paz
2010-2016
We recently identified the thalamic dopaminergic system in human and macaque monkey brains, and, based on earlier reports paucity of dopamine rat thalamus, hypothesized that this was particularly developed primates. Here we test hypothesis using immunohistochemistry against transporter (DAT) adult brains. The extent density DAT-immunoreactive (-ir) axons were remarkably greater dorsal where mediodorsal association nucleus ventral motor nuclei held densest immunolabeling. In contrast, sparse...
The glutamate–glutamine cycle faces a drain of glutamate by oxidation, which is balanced the anaplerotic synthesis and glutamine in astrocytes. De novo astrocytes requires an amino group whose origin unknown. deficiency Aralar/ AGC1, main mitochondrial carrier for aspartate–glutamate expressed brain, results drastic fall brain production but modest decrease levels, not due to decreases neuronal or synaptosomal content. In vivo 13 C nuclear magnetic resonance labeling with 2 acetate (1- C)...
Abstract Opiate‐evoked reward and motivated behaviors reflect, in part, the enhanced release of dopamine produced by activation μ‐opioid receptor (μOR) ventral tegmental area (VTA). We examined functional sites for μOR potential interactions with dopaminergic neurons within rat VTA using electron microscopy immunocytochemical localization antipeptide antisera raised against tyrosine hydroxylase (TH), synthesizing enzyme catecholamines. The cellular subcellular distribution was remarkably...
Hypocretins or orexins (Hcrt/Orx) are hypothalamic neuropeptides that synthesized by neurons located mainly in the perifornical area of posterolateral hypothalamus. These origin an extensive and divergent projection system innervating numerous structures central nervous system. In recent years it has become clear these involved regulation many organic functions, such as feeding, thermoregulation neuroendocrine cardiovascular control, well control sleep-wakefulness cycle. this respect,...
The Hypocretin1/OrexinA (Hcrt1/OxA) neuropeptides are found in a group of posterolateral hypothalamus neurons and involved sleep-wakefulness cycle regulation. Hcrt1/OxA project widely to brainstem aminergic structures, such as the locus coeruleus (LC), which maintenance wakefulness EEG activation through intense projections medial prefrontal cortex (mPFC). Moreover, defects system linked narcolepsy, disorder characterized by excessive diurnal hypersomnia REM state disturbance. We aimed...
Abstract Mesocorticolimbic projections originating from dopaminergic and GABAergic neurons in the ventral tegmental area (VTA) play a critical role opiate addiction. Activation of μ‐opioid receptors (MOR), which are located mainly within inhibitory VTA, results enhanced transmission target regions, including medial prefrontal cortex (mPFC). We combined retrograde tract‐tracing electron microscopic immunocytochemistry to determine if VTA that project mPFC contain MOR or receive input...
Abstract The perifornical (PeF) area in the posterior lateral hypothalamus has been implicated several physiological functions including regulation of sleep–wakefulness. Some PeF neurons, which contain hypocretin, have suggested to play an important role sleep–wake regulation. aim present study was examine effect and hypocretin on electrophysiological activity neurons oral pontine reticular nucleus (PnO), is structure generation maintenance rapid eye movement sleep. PnO were recorded...
Abstract Hypocretinergic/orexinergic neurons, which are known to be implicated in narcolepsy, project the pontine tegmentum areas involved control of rapid eye movement (REM) sleep. Here, we report effects on sleep‐wakefulness produced by low‐volume microinjections hypocretin (Hcrt)1 (20–30 nL, 100, 500 and 1000 μ m ) carbachol 0.1 delivered two oral free‐moving cats with electrodes for chronic sleep recordings: dorsal (DOPT) ventral part reticular nucleus (vRPO). Carbachol DOPT dissociate...
Polymorphisms in the region of calmodulin-dependent kinase isoform D (CaMK1D) gene are associated with increased incidence diabetes, most common polymorphism resulting recognition by transcription factors and protein expression. While reducing CaMK1D expression has a potentially beneficial effect on glucose processing human hepatocytes, there no known selective inhibitors CaMK1 kinases that can be used to validate or translate these findings. Here we describe development series potent,...
The perifornical area in the posterior lateral hypothalamus (PeFLH) has been implicated several physiological functions including sleep-wakefulness regulation. PeFLH contains cell types those expressing orexins (Orx; also known as hypocretins), mainly located PeF nucleus. aim of present study was to elucidate synaptic interactions between Orx neurons and different brainstem involved generation wakefulness sleep stages such locus coeruleus (LC) nucleus (contributing wakefulness) oral pontine...
Muscarinic modulation of mesolimbic dopaminergic neurons in the ventral tegmental area (VTA) plays an important role reward, potentially mediated through M5 muscarinic acetylcholine receptor (M5R). However, key sites for M5R-mediated control dopamine within this region are still unknown. To address question we examined electron microscopic immunocytochemical localization antipeptide antisera against M5R and plasmalemmal transporter (DAT) single sections rat VTA. was located mainly to VTA...
Abstract Acetylcholine can affect cognitive functions and reward, in part, through activation of muscarinic receptors the ventral tegmental area (VTA) to evoke changes mesocorticolimbic dopaminergic transmission. Among known receptor subtypes present VTA, M2 (M2R) is most implicated autoregulation also may play a heteroreceptor role regulation output neurons. We sought determine functionally relevant sites for M2R relation VTA neurons by examining electron microscopic immunolabeling dopamine...
Cortical activation and goal-directed behaviors characterize wakefulness. One cortical region especially involved in these phenomena is the medial prefrontal cortex (mPFC), which receives many inputs from cholinergic-containing neurons brain stem structures implicated arousal wakefulness, such as laterodorsal tegmental nucleus (LDT). Hypocretins/orexins (Hcrt/Ox), whose dysfunction linked to narcolepsy, maintains stabilizes sleep-wakefulness states. We aim determine if Hcrt1/OxA axons (1)...
Hypocretins/orexins (Hcrt/Ox) are hypothalamic neuropeptides involved in sleep-wakefulness regulation. Deficiency Hcrt/Ox neurotransmission results the sleep disorder narcolepsy, which is characterized by an inability to maintain wakefulness. The neurons maximally active during wakefulness and project widely ventral tegmental area (VTA). A dopamine-containing nucleus projecting extensively cerebral cortex, VTA enhances In present study, we used retrograde tracing from medial prefrontal...
There is little information on either the transition state occurring between slow-wave sleep (SWS) and rapid eye movement (REM) sleep, as well about its neurobiological bases. This state, which known intermediate (IS), well-defined in rats but poorly characterized cats. Previous studies our laboratory demonstrated that cholinergic stimulation of perilocus coeruleus α nucleus (PLCα) pontine tegmentum cats induced two states: wakefulness with muscle atonia a dissociated we have called SPGO...