A5085964213- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Endoplasmic Reticulum Stress and Disease
- RNA regulation and disease
- Heat shock proteins research
- Enzyme Structure and Function
- NF-κB Signaling Pathways
- Genetic Neurodegenerative Diseases
- Autophagy in Disease and Therapy
- Invertebrate Immune Response Mechanisms
- Protein Tyrosine Phosphatases
- Viral gastroenteritis research and epidemiology
- Cancer-related Molecular Pathways
- Cell death mechanisms and regulation
- Cellular transport and secretion
- Insect Resistance and Genetics
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Insect-Plant Interactions and Control
- Protein purification and stability
Universidade Nova de Lisboa
1996-2024
Instituto de Tecnología Química
2024
Instituto Português Da Qualidade
2010
Université Toulouse III - Paul Sabatier
2009
Centre National de la Recherche Scientifique
2009
Summary In this paper we show that RNase R is a cold shock protein induced seven‐ to eightfold by and its expression tightly regulated temperature. Transcriptional studies reveal the rnr gene co‐transcribed with flanking genes as an operon under shock. The induction of levels mainly result stabilization transcripts. transient stability transcripts shown be PNPase at end acclimation phase. Studies mutant revealed cold‐shock phenotype showing contributes growth low temperatures. We have can...
In nature, bacteria remain mostly in the stationary phase of life cycle. Although mRNA is a major determinant gene expression, little known about decay phase. The results presented herein demonstrate that RNase R induced and involved post-transcriptional regulation ompA mRNA. This work first report activity on full length absence single rnr mutant, higher levels are found as consequence stabilization transcript. effect growth-phase-specific not growth-rate-dependent event. These were...
Abstract The unfolded protein response (UPR) maintains homeostasis of the endoplasmic reticulum (ER). Residing in ER membrane, UPR mediator Ire1 deploys its cytoplasmic kinase-endoribonuclease domain to activate key transcription factor Xbp1 through non-conventional splicing mRNA. also degrades diverse ER-targeted mRNAs regulated Ire1-dependent decay (RIDD), but how it spares mRNA from this is unknown. Here, we identify binding sites for RNA-binding Pumilio 3′UTR Drosophila . In developing...
Summary PNPase and RNase II are the key regulatory exo‐nucleases controlling mRNA decay in Escherichia coli The rnb transcripts were found to proceed through terminator was be involved 3’to 5’degradation of mRNA. Analysis these longer 3’termini revealed that they located UA‐rich regions. Comparison single double mutants suggested could have different roles degradation unstructured We shown levels can vary over a fivefold range haploid cells its expression depends on levels. PNPase‐deficient...
In Escherichia coli , ribonucleases are effectors that rapidly modulate the levels of mRNAs for adaptation to a changing environment. Factors involved in regulation these can be relevant mRNA stability. RNase II is one main responsible exonucleolytic activity E. extracts. We have identified and characterized new gene, which was named gmr ( g ene m odulating R Nase II). The results demonstrate deletion associated with changes activity. Western analysis exoribonuclease assays showed threefold...
In mammals, AU-rich elements (AREs) are critical regulators of mRNA turnover. They recruit ARE-binding proteins that inhibit or stimulate rapid degradation in response to stress developmental cues. Using a bioinformatics approach, we have identified AREs Drosophila melanogaster 3' untranslated regions and validated their cross-species conservation distant genomes. We generated ARE database (D-ARED) established about 16% D. genes contain the mammalian signature, an AUUUA pentamer A/U-rich...
Abstract The major 3′–5′ pathway of RNA degradation in eukaryotic cells involves the exosome, which is a multi‐protein complex exoribonucleases. exoribonucleases within this are highly conserved and closely related to prokaryotic ribonucleases. We have identified characterised expression pattern Drosophila tazman ( taz ), component exosome Escherichia coli RNaseR yeast Rrp44p. transcripts differentially expressed during development, with maximum levels 6–8 hr embryos. In situ hybridisation...
RNA degradation is important in the post-transcriptional control of gene expression. The processing, and quality performed by many different classes ribonucleases. Ribonuclease II (RNase II) a 643-amino-acid enzyme that degrades single-stranded from its 3′-end, releasing ribonucleoside 5′-monophosphates. RNase was expressed both as wild type D209N mutant form. latter also produced an SeMet derivative. various protein forms were crystallized using vapour-diffusion method. Wild-type two...
The Unfolded Protein Response (UPR) is composed by homeostatic signaling pathways that are activated the accumulation of misfolded proteins in Endoplasmic Reticulum (ER), a condition known as ER stress. Prolonged stress and activation UPR causes cell death, mechanisms remain poorly understood. Here, we report regulation Ataxin-2 Fbxo42 crucial step during UPR-induced death. From genetic screen Drosophila, identified loss function mutations suppress death retinal degeneration induced...
SUMMARY The unfolded protein response (UPR) maintains homeostasis of the endoplasmic reticulum(ER). Residing in ER membrane, UPR mediator Ire1 deploys its cytoplasmic kinase-endoribonuclease domain to activate key transcription factor Xbp1 through non-conventional splicing mRNA. also degrades diverse ER-targeted mRNAs regulated Ire1-dependent decay (RIDD), but how it spares mRNA from this is unknown. We identified binding sites for RNA-binding Pumilio 3’UTR Drosophila Xbp1. In developing eye...