A5103054815- Genetic and phenotypic traits in livestock
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Glioma Diagnosis and Treatment
- Mycobacterium research and diagnosis
- Helicobacter pylori-related gastroenterology studies
- Cancer, Hypoxia, and Metabolism
- ATP Synthase and ATPases Research
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Environmental DNA in Biodiversity Studies
- Genetic Mapping and Diversity in Plants and Animals
- MicroRNA in disease regulation
- Nutrition, Genetics, and Disease
- Genetic diversity and population structure
- RNA modifications and cancer
Hormel (United States)
2024-2025
University of Minnesota
2024-2025
University of Vermont
2018-2023
Heterozygous histone H3.3K27M mutation is a primary oncogenic driver of Diffuse Midline Glioma (DMG). inhibits the Polycomb Repressive Complex 2 (PRC2) methyltransferase activity, leading to global reduction and redistribution repressive H3 lysine 27 tri-methylation (H3K27me3). This epigenomic rewiring thought promote gliomagenesis, but precise role K27M in gene regulation tumorigenesis remains incompletely understood. We established isogenic DMG patient-derived cell lines using CRISPR-Cas9...
The Ovine Functional Annotation of Animal Genomes (FAANG) project, part the broader livestock species FAANG initiative, aims to identify and characterize gene regulatory elements in domestic sheep. Regulatory element annotation is essential for identifying genetic variants that affect health production traits this important agricultural species, as greater than 90% underlying effects are estimated lie outside transcribed regions. Histone modifications distinguish active or repressed...
Abstract Background Heterozygous histone H3.3K27M mutation is a primary oncogenic driver of Diffuse Midline Glioma (DMG). inhibits the Polycomb Repressive Complex 2 (PRC2) methyltransferase complex, leading to global reduction and redistributing repressive H3 lysine 27 tri-methylation. This rewiring epigenome thought promote gliomagenesis. Methods We established novel, isogenic DMG patient-derived cell lines that have been CRISPR-Cas9 edited H3.3 WT or alone in combination with EZH2 EZH1...
Johne’s Disease (JD), caused by Mycobacterium avium subsp paratuberculosis (MAP), is an incurable disease of ruminants and other animal species characterized imbalance gut immunity. The role MAP infection on the epigenetic modeling immunity during progression JD still unknown. This study investigated DNA methylation patterns in ileal (IL) lymph node (ILLN) tissues from cows diagnosed with persistent subclinical over a one to 4 years period. samples IL ILLN negative (MAPneg) ( n = 3) or...
Abstract Genetic diversity is critical to a population’s ability overcome gradual environment change. Large-bodied wildlife existing in regions with relatively high human population density are vulnerable isolation-induced genetic drift, bottlenecks, and loss of diversity. Moose ( Alces americanus ) eastern North America have complex history drastic changes. Current potential threats moose populations this region could be exacerbated by connectivity among subpopulations. Existing diversity,...
The 5’AMP-activated protein kinase (AMPK) gene family comprises an evolutionary conserved serine/threonine heterotrimeric kinase. primary function of this is regulation cellular energy and metabolism. activation primarily occurs when there a depletion ATP due to environmental nutritional stressors, which associated with rise AMP levels. Subsequently, the genes initiate energy-conserving measures within cell protect it from decrease in these has been linked epigenetic mechanism methylation....
Abstract Diffuse midline glioma (DMG), a highly aggressive pediatric brain cancer, and glioblastoma multiforme (GBM), an intractable disease, pose significant clinical challenges. DMG is characterized by dishearteningly short median survival period of 15 months, GBM low five-year rate merely 6.9 percent. A critical player in these malignancies Enhancer zeste homologue 2 (EZH2), subunit the polycomb repressive complex (PRC2) that catalyzes histone 3 Lysine 27 tri-methylation (H3K27Me3). This...
Abstract Background: Diffuse Midline Glioma (DMG), a severe pediatric brain tumor, is characterized by its aggressive nature and poor prognosis. Common in midline structures like the thalamus brainstem, DMGs often harbor H3K27M mutations, leading to reduced histone trimethylation at lysine 27 loss of serine 31 phosphorylation. Building on these findings, our laboratory has undertaken novel approach engineering knockout Polycomb Repressive Complex 2 (PRC2) selected cell lines. This was...
Abstract Heterozygous H3.3 K27M mutations are central to the pathogenesis of diffuse midline gliomas (DMG), causing a global reduction in H3K27 trimethylation (H3K27me3) and subsequent epigenetic dysregulation. contains unique Serine 31 (S31) phosphorylation site crucial for mitotic checkpoint regulation. In DMG cells, reduced S31 correlates with chromosomal instability aneuploidy. Using mouse model, we demonstrate that both H3.3K27M H3.3S31A promote high-grade glioma development, whereas...
Abstract Johne’s Disease (JD), caused by Mycobacterium avim spp paratuberculosis (MAP), is a chronic and incurable disease of ruminants with devastating consequences to the dairy industry. MAP can alter expression genes biological processes during progression JD. While some studies have examined role gene regulators like microRNA in pathogenesis JD, no study has explored DNA methylation. This therefore effect on methylation profile ileum lymph node (ILLN) cows subclinical infection. from...
Abstract Several investigations on disease progression of Mycobacterium avium subsp. paratuberculosis (MAP) infection in dairy cows have revealed molecular mechanisms including genes and pathways implicated MAP pathogenesis. Epigenetic processes DNA methylation are known to regulate the expression many biological processes. However, limited studies examined role pathogenesis Johne’s (JD). This study impact subclinical profile ileum cows, site initial interaction between host. from tissues...
Abstract Characterizing the locations of genetic regulatory elements is critical for understanding mechanisms complex phenotypic traits related to production and health in livestock species. The Ovine Functional Annotation Animal Genomes (FAANG) Project aims characterize transcriptional across sheep genome facilitate a better biological influencing sheep. Assays including sequencing messenger RNA (mRNA-seq), cap analysis gene expression (CAGE), chromatin immunoprecipitation histones...