A5023012124- Marine Sponges and Natural Products
- Microbial Natural Products and Biosynthesis
- Synthesis and Biological Activity
- Marine Biology and Environmental Chemistry
- Lepidoptera: Biology and Taxonomy
- Spider Taxonomy and Behavior Studies
- Coral and Marine Ecosystems Studies
- Leech Biology and Applications
- Phytochemical compounds biological activities
- Aquaculture disease management and microbiota
- Carbohydrate Chemistry and Synthesis
- Myxozoan Parasites in Aquatic Species
- Venomous Animal Envenomation and Studies
- Traditional and Medicinal Uses of Annonaceae
- Chemical synthesis and alkaloids
- Synthetic Organic Chemistry Methods
- Entomological Studies and Ecology
- Beetle Biology and Toxicology Studies
- Echinoderm biology and ecology
- Natural product bioactivities and synthesis
- Synthesis and Characterization of Pyrroles
- Advanced Chemical Sensor Technologies
- Metabolomics and Mass Spectrometry Studies
- Amphibian and Reptile Biology
- Microbial infections and disease research
University of South Florida
2024-2025
Ollscoil na Gaillimhe – University of Galway
2017-2024
New technology has opened opportunities for research and exploration of deep-water ecosystems, highlighting deep-sea coral reefs as a rich source novel bioactive natural products. During our ongoing investigation the chemodiversity Irish deep sea soft Anthothela grandiflora, we report 12 unreported cadinene-like functionalized sesquiterpenes, anthoteibinenes F–Q. The metabolites were isolated using both bioassay- 1H NMR-guided approaches. One-/two-dimensional NMR spectroscopy high-resolution...
Since the introduction of online open-source GNPS, molecular networking has quickly become a widely applied tool in field natural products chemistry, with applications from dereplication, genome mining, metabolomics, and visualization chemical space. Studies have shown that data dependent acquisition (DDA) parameters affect network topology but are limited number studied. With an aim to optimize LC-MS2 for integrating GNPS-based into our marine workflow, design experiment (DOE) was used...
Abstract The false widow spider Steatoda nobilis is associated with bites which develop bacterial infections that are sometimes unresponsive to antibiotics. These could be secondary derived from opportunistic bacteria on the skin or directly vectored by spider. In this study, we investigated whether it plausible for S. and other synanthropic European spiders vector during a bite, seeking identify pathogenic potential spiders. 11 genera of were identified through 16S rRNA sequencing body...
Chemical investigation of extracts from the Irish deep-sea soft coral Anthothela grandiflora revealed cadinene-like sesquiterpenes, anthoteibinenes A–E, bearing unusual dimethylamine substitution. Structure elucidation was accomplished using 1D/2D NMR spectroscopy and high-resolution mass spectrometry, while NOESY experiments, gauge invariant atomic orbital (GIAO) calculations coupled with DP4+ probabilities measures, ECD comparisons were incorporated to propose their relative absolute...
Objective: The noble false widow Steatoda nobilis is the only medically significant spider known to occur in British Isles and Ireland, with a single case of steatodism ever reported from Great Britain. We present here five new cases envenomations by S. nobilis, three Ireland two Britain describe symptoms not previously for genus Steatoda.Case presentation: Four adult males one female confirmed bites their authors. General practitioner chart was obtained #3. In all cases, were immediately...
Chronic diseases characterized by bone and cartilage loss are associated with a reduced ability of progenitor cells to regenerate new tissues in an inflammatory environment. A promising strategy treat such is based on tissue repair mediated human mesenchymal stem (hMSCs), but therapeutic outcomes hindered the absence small molecules efficiently modulate cell behaviour. Here, we applied high-throughput drug screening technology bioprospect large library extracts from Irish deep-sea organisms...
The chemical investigation of marine invertebrates from the deep Northeastern Atlantic revealed new lipoglycotripeptides named characellides isolated tetractinellid sponge Characella pachastrelloides. This family natural products features a central tripeptide linked to rare sugar unit and long alkyl chain ending with 2,3-dimethyltetrahydropyran. configurations all 13 chiral centers were determined by extensive use NMR data circular dichroism spectra combined calculations.
As exploration of ocean depths >1000 m is only possible by expensive remotely operated underwater vehicles, deep-sea invertebrates represent a largely untapped source marine metabolites for potential applications in medicine. Our current study aims to investigate these Ireland discover new biological and chemical diversity. Here, we the bubble gum coral,
Sponges are at the forefront of marine natural product research. In deep sea, extreme conditions have driven secondary metabolite pathway evolution such that we might expect deep-sea sponges to yield a broad range unique products. Here, investigate chemodiversity tetractinellid sponge, Characella pachastrelloides, collected from ~800 m depth in Irish waters. First, analyzed MS/MS data obtained fractions this sponge on GNPS public online platform guide our exploration its chemodiversity....
Shallow water black corals were harvested as long ago the ancient Greek civilization, for which they used medicines and jewelry. More recently, international conservation measures have been enacted to preserve their habitats due overharvesting. Medicinal usage of may stem from need chemical defenses withstand predation since are unable withdraw polyps. With aim discovering novel anti-cancer drugs increase conservational awareness these organisms, deep-sea Irish continental margin screened...
New technology has opened opportunities for research and exploration of deep-water ecosystems, highlighting deep-sea coral reefs as a rich source novel bioactive natural products. During our ongoing investigation the chemodiversity Irish soft Anthothela grandiflora, we report 12 unreported cadinene-like functionalized sesquiterpenes, anthoteibinenes F-Q. The metabolites were isolated using both bioassay- 1H NMR-guided approaches. 1D/2D NMR spectroscopy high-resolution mass spectrometry used...
The noble false widow Steatoda nobilis (Thorell, 1875) has established thriving populations in urban centres throughout England and Wales since it was accidentally imported from the Canary Islands Madeira to Britain over a century ago. In recent years, colonised parts of Western Europe, California, Chile Middle East. Ireland, first recorded 1999 single location Co Wicklow. present study examines current range main habitats Ireland assesses its potential as an invasive species by documenting...
Marine sponges and their associated microbiota are multicellular animals known to produce metabolites with interesting pharmacological properties playing a pivotal role against plethora of pathologic disorders such as inflammation, cancer infections. Characellide A B belong novel class glycolipopeptides isolated from the deep sea marine sponge Characella pachastrelloides. In this study, we have evaluated effects characellide on cytokine chemokine release human peripheral blood mononuclear...