A5006522331- Food Allergy and Anaphylaxis Research
- CRISPR and Genetic Engineering
- Studies on Chitinases and Chitosanases
- Animal Genetics and Reproduction
- Allergic Rhinitis and Sensitization
- Transgenic Plants and Applications
- Enzyme Production and Characterization
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Biochemical and Structural Characterization
- Insect Resistance and Genetics
- Monoclonal and Polyclonal Antibodies Research
- Vehicular Ad Hoc Networks (VANETs)
- Chromium effects and bioremediation
- Microbial Metabolites in Food Biotechnology
- Legume Nitrogen Fixing Symbiosis
- Hormonal and reproductive studies
- Nematode management and characterization studies
- Agricultural Practices and Plant Genetics
- Microbial Fuel Cells and Bioremediation
- Microbial Community Ecology and Physiology
- Advanced Authentication Protocols Security
- Cancer Risks and Factors
- Sugarcane Cultivation and Processing
- Insect Utilization and Effects
Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies
2014-2025
National Research Council
2015-2025
Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico
2024-2025
University of Milan
2023-2025
European Institute of Oncology
2025
Roma Tre University
2023-2025
University of Bari Aldo Moro
2008-2019
Light-harvesting complex II (LHCII) contains three highly homologous chlorophyll-a/b-binding proteins (Lhcb1, Lhcb2 and Lhcb3), which can be assembled into both homo- heterotrimers. Lhcb1 are reversibly phosphorylated by the action of STN7 kinase PPH1/TAP38 phosphatase in so-called state-transition process. We have developed antibodies that specific for forms Lhcb2. found is more rapidly than Lhcb1: 10 sec 'state 2 light' results phosphorylation to 30% maximum level. Phosphorylated...
The endometrium is a challenging site for metagenomic analysis due to difficulties in obtaining uncontaminated samples and the limited abundance of bacterial population. Indeed, solid correlations between endometrial physio-pathologic conditions bacteria compositions have not yet been firmly established. Nevertheless, study microbiota great interest close profiles, women’s health, successful pregnancies. In this study, we decided tackle through population women subjected elective caesarean...
Abstract The replication of Coronaviridae viruses depends on the synthesis structural proteins expressed through discontinuous transcription subgenomic RNAs (sgRNAs). Thus, detecting sgRNAs, which reflect active viral replication, provides valuable insights into infection status. Current diagnostic methods, such as PCR‐based assays, often involve high costs, complex equipment, and reliance highly trained personnel. Additionally, their specificity can be compromised by technical limitations...
Microorganisms inhabiting saline environments are an interesting ecological model for the study of adaptation organisms to extreme living conditions and constitute a precious resource enzymes bioproducts biotechnological applications. We analyzed microbial communities in nine ponds with increasing salt concentrations (salinity range 4.9-36.0%) Saltern Margherita di Savoia (Italy), largest thalassohaline saltern Europe. A deep-metabarcoding NGS procedure addressing separately V5-V6 V3-V4...
Rhodobacter sphaeroides has for a long time been investigated its adaptive capacities to different environmental and nutritional conditions, including presence of heavy metals, which make it valuable model organism understanding bacterial adaptation metal stress conditions future applications, such as bioremediation polluted sites. To further characterize the capability R. cope with high cobalt ion concentrations, we combined selection defective mutants, carried out by negative transposon...
The artificial introduction in the soil of antagonistic microorganisms can be a successful strategy, alternative to agrochemicals, for control root-knot nematodes (Meloidogyne spp.) and preserving plant health. On other hand, roots associated rhizosphere constitute complex system which contribution microbial community is fundamental health development, since microbes may convert organic inorganic substances into available nutrients. In present study, potential nematicidal activity...
Determination of nucleotide sequences adjacent to a known region is recurring need in many genome scale studies. Various methods have been developed based on PCR techniques order fulfill this aim and overcome the time-consuming approach screening genomic libraries. Usually these protocols rely specific requirements strategies, such as presence suitable restriction sites ligation single- or double-strand linkers, thus limiting their application certain extent. In paper we present an...
Genome Walking (GW) comprises a number of PCR-based methods for the identification nucleotide sequences flanking known regions. The different have been used several purposes: from de novo sequencing, useful unknown regions, to characterization insertion sites viruses and transposons. In latter cases recently boosted by coupling Next Generation Sequencing technologies. This review will focus on development protocols application (NGS) technologies GW, which developed in course analysis...
Background: The endometrium remains a difficult tissue for the analysis of microbiota, mainly due to low bacterial presence and sampling procedures. Among its pathologies, endometrial cancer has not yet been completely investigated relationship with microbiota composition. In this work, we report on possible correlations between dysbiosis cancer. Methods: Women at various stages tumor progression were enrolled together women benign polymyomatous uterus as control. Analyses performed using...
Sucrose transport is the central system for allocation of carbon resources in vascular plants. synthase (SUS), which reversibly catalyzes sucrose synthesis and cleavage, represents a key enzyme control flow into starch biosynthesis. In present study genomic identification characterization Sus2-2A Sus2-2B genes coding SUS durum wheat (cultivars Ciccio Svevo) reported. The were analyzed their expression different tissues at seed maturation stages, four tetraploid genotypes (Svevo, Ciccio,...
Food allergies are recognized as an increasing health concern. Proteins commonly identified food allergens tend to have one of about 30 different biochemical activities. This leads the assumption that must specific structural features which causes their allergenicity. But these not completely understood. Uncovering basis allergenicity would allow improved diagnosis and therapy provide insights for safer production. The availability recombinant can accelerate analysis benefit studies in...
Nickel acts as cofactor for a number of enzymes many bacteria species. Its homeostasis is ensured by proteins working ion efflux or accumulation systems. These mechanisms are also generally adopted to counteract life-threatening high extra-cellular Ni2+ concentrations. Little known regarding nickel tolerance in the genus Sphingobium. We studied response novel Sphingobium sp. ba1 strain, able adapt Differential gene expression cells cultured 10 mM Ni2+, investigated RNA-seq analysis,...
The progressive improvement of lymphoma treatment has led to an important prolongation patient survival and life expectancy. principal international scientific societies oncology now therefore recommend that long-term survivors join fertility programs. Specifically, fertile-age patients should be assisted by a multidisciplinary team, including specialists dedicated preservation in oncology, order support the completion their reproductive project. In general population, use Myo-Inositol...