The effect of acute exercise at three graded intensities on plasma growth hormone (GH) and prolactin (PRL) concentrations was examined in groups healthy male volunteers. According to their training status these subjects were divided into untrained, moderately trained highly trained. A clear response GH registered already an intensity 50% maximal oxygen uptake (Vo2max) with a 70% Vo2max no further 90% Vo2max. In contrast, PRL observed Vo2max, small rise seen the highest occurred Basal...

The successful therapeutic use of interferon-α (IFN-α) in myeloproliferative disorders offered the possibility to test its acute and long-term effects on hypothalamic-pituitary-adrenal (HPA) axis humans. ACTH cortisol plasma concentrations were measured 8 patients hourly starting from 4 p.m. through 12 three occasions. first time all studied before initiation therapy, when vehicle was injected alone. again day 1 IFN-α therapy (5 million units) once more after 3 weeks therapy. On control day,...

To assess the effect of chronically elevated plasma growth hormone (GH) levels on bone metabolism and mineral density (BMD), 16 patients (10 females 6 males) with a mean age 49.1 +/- 13.2 years (range 33-68) active acromegaly were studied compared to control group sex- age-matched subjects. BMD lumbar spine two different sites proximal femur measured by dual-energy x-ray absorptiometry (Norland XR-26). In acromegalic GH concentration was 30.1 11.1 micrograms/liter, somatomedin C (SMC) 6.5...

Lupins, which belong to the family of fabaceae, are plants with a high nutritional potential due their protein content, symbiotic nitrogen fixation and environmental adaptability. Yet, plant pathogens including Fusairum oxysporum f. sp. lupini considerable problem in lupin cultivation as they can cause yield reduction. To find Lupinus angustifolius accessions enhanced resistance towards Fusarium infection, we screened 20 yet uncharacterized root infection assay. The infected roots were...

Fusarium is a genus that mostly consists of plant pathogenic fungi which are able to produce broad range toxic secondary metabolites. In this study, we focus on type A trichothecene-producing isolate (15-39) sporotrichioides from Lower Austria. We assessed the metabolite profile and optimized toxin production conditions autoclaved rice found in addition large amounts T-2 HT-2 toxins, strain was HT-2-glucoside. The optimal for toxin, HT-2-glucoside were incubation at 12 °C under constant...

Fusarium graminearum is a plant pathogenic fungus which able to infect wheat and other economically important cereal crop species. The role of ethylene in the interaction with host plants unclear controversial. We have analyzed inventory genes putative function production or degradation precursor ACC. F. graminearum, contrast species, does not contain candidate gene encoding ethylene-forming enzyme. Three similarity ACC synthases exist; heterologous expression these did reveal enzymatic...

The emerging mycotoxin fusaproliferin is produced by Fusarium proliferatum and other related species. Several fungi from taxonomic groups were also reported to produce or the deacetylated derivative, known as siccanol terpestacin. Here, we describe identification functional characterization of genes encoding biosynthetic enzymes: a terpenoid synthase, two cytochrome P450s, FAD-oxidase an acetyltransferase. With exception one gene CYP450 (FUP2, FPRN_05484), knock-out mutants candidate could...

Over the past decades, strong evidence has accumulated that growth hormone (GH) immunostimulatory properties. Therefore, an investigation was conducted on 10 acromegalic patients and 9 age- sex-matched healthy controls to determine whether plasma GH concentrations correlate with changes in several immune parameters, including serum of immunoglobulins, lymphocyte subsets, transformation phytohemagglutinin (PHA), natural killer (NK) cell activity as well phagocytic metabolic burst activity....

In vitro and in vivo studies rodents human suggested an immunostimulatory effect of prolactin. The aim the present study was to determine impact chronically elevated serum prolactin concentrations on immune system patients with prolactinomas. For this purpose parameters humoral cellular were studied seven prolactinomas two occasions (1) when their concentration had been normalized through treatment dopamine agonists (2) high. Serum immunoglobulines, interleukin 1, 3 6, TNF-alpha,...

Eleven patients (6 men, 5 women, mean age 42 [25-65] years) with prolactinomas were treated for 4-16 months a new dopamine agonist (CV 205-502), at daily dose of between 0.075 and 0.45 mg. All had previously undergone surgery and/or been bromocriptine or lisuride, but not tolerated this therapy well, shown sufficient suppression serum prolactin levels. Baseline levels 149 4120 ng/ml. During treatment, fell significantly in all patients, to 2.0 683 ng/ml, within the reference range five...

Abstract Proximity labelling that uses promiscuous biotin ligases (BirA) fused to a bait protein is powerful tool identify interaction partners in vivo under different metabolic or developmental conditions. BirA can also be used determine composition and at specific chromatin locations when it with enzymatically-disabled Cas9 (dCas9) then guided the location of interest by sgRNAs. We adapted this method (called CasID) for fungal cells using nitrate assimilation gene cluster A. nidulans as...

Abstract Plant pathogenic fungi have evolved different strategies to interfere with plant defense mechanisms. The well described fungal pathogen Fusarium graminearum is not only able produce trichothecene toxins like deoxynivalenol, but also the hormone auxin. Highly elevated levels of auxin and derivatives such as IAA-glucoside or IAA amino-acid conjugates were observed in wheat cultivar Apogee infected F. . We report that cleave tryptamine-derived hydroxycinnamic acid amides, e.g. compound...

Plasma concentrations of Epidermal Growth Factor (EGF) in healthy control persons do not change significantly during adult lifetime and no diurnal changes could be detected. In addition, alterations plasma EGF occur acute chronic stress all three trimesters pregnancy. Thus, the previously suggested theories that might play a causative role activation hypothalamic-pituitary adrenal axis reaction prevention peptic ulcers pregnancy confirmed by present data.

Trichothecenes are a class of structurally diverse mycotoxins with more than 200 naturally occurring compounds. Previously, new compound, pentahydroxyscirpene (PHS), was reported as byproduct nivalenol producing Fusarium strain, IFA189. PHS contains hydroxy group at C-8 instead the keto type B trichothecenes. In this work, we demonstrate that IFA189 belongs to species kyushuense using molecular tools. Production in vitro also observed for several isolates other nivalenol. Furthermore, report...

It was the aim of this study to test for a possible effect new non ergot dopamine agonist CV 205-502 on plasma growth hormone (GH) concentrations in acromegaly. 10 acromegalic patients received single oral dose 150 micrograms after an overnight fast. As control group 7 undertook same procedure without receiving any drug. Blood samples were drawn hourly up hours thereafter determination GH. Plasma decreased by 48.8 +/- 8.7%. The nadir observed 3 administered and GH remained suppressed...