Abstract Solid-state nanopores allow high-throughput single-molecule detection but identifying and even registering all translocating small molecules remain key challenges due to their high translocation speeds. We show here the same electric field that drives into pore can be redirected selectively pin delay transport. A thin high-permittivity dielectric coating on bullet-shaped polymer permits leakage at tip produce a voltage-dependent surface entry side reversibly edge-pin molecules. This...

Abstract Over the past two decades, dynamic DNA origami structures have emerged as promising candidates for nanoscale signal and cargo transport. walkers, programmable nanostructures that traverse tracks made of DNA, represent a key innovation in this field, enabling controlled directional movement at nanoscale. Despite relatively fast diffusion rates, speed walkers remains constrained by reaction-limited nature strand exchange mechanisms, which depend both on foothold-walker affinity...

The rate and extent of drug release under physiological conditions is a key factor influencing the therapeutic activity formulation. Real-time detection by conventional pharmacokinetics approaches confounded low sensitivity, particularly in case tissue-targeted novel delivery systems, where concentrations reach systemic circulation. We present fluorescence turn-on platform for real-time monitoring from nanoparticles based on reversible quenching fluorescein esters. Fluorescein-conjugated...

The presence of a small number (∼1000) charged nanoparticles or macromolecules on the surface an oppositely perm-selective membrane is shown to sensitively gate ionic current through at particular voltage, thus producing voltage signal much larger than thermal noise. We show that, sufficiently high voltages, vortices appear and sustain ion-depleted boundary layer that controls diffusion length ion current. An asymmetric vortex bifurcation occurs beyond critical reduce differential resistance...

Tethered motion is ubiquitous in nature, offering controlled movement and spatial constraints to otherwise chaotic systems. The enhanced functionality practical utility of tethers has been exploited biotechnology, catalyzing the design novel biosensors molecular assembly techniques. While notable technological advances incorporating tethered motifs have made, a theoretical gap persists within paradigm, hindering comprehensive understanding tethered-based technologies. In this work, we focus...

Biomolecular nanotechnology has helped emulate basic robotic capabilities such as defined motion, sensing, and actuation in synthetic nanoscale systems. DNA origami is an attractive approach for nanorobotics, it enables creation of devices with complex geometry, programmed rapid actuation, force application, various kinds sensing modalities. Advanced functions like feedback control, autonomy, or routines also require the ability to transmit signals among subcomponents. Prior work established...

The evolution of dynamic DNA nanostructures has propelled nanotechnology into a robust and versatile field, offering groundbreaking applications in nanoscale communication, drug delivery, molecular computing. Yet, the full potential this technology awaits further enhancement through optimization kinetic properties governing conformational changes. In work, we introduce mean-field theory to characterize behavior origami hinge where each arm bears complementary single-stranded overhangs...

We verify both theoretically and by simulation that an AC electric field, with a frequency much higher than the dissociation rate, can significantly accelerate rate of biological molecules under isothermal conditions. The cumulative effect field is shown to break key bottleneck reducing entropy (and increasing free energy local minimum) via alignment molecular dipole field. For frequencies below resonant which corresponds inverse Debye relaxation time, be accelerated factor scales as...

Molecular systems with ability to controllably transform between different conformations play pivotal roles in regulating biochemical functions. Here, we report the design of a bistable DNA origami four-way junction (DOJ) molecular system that adopts two distinct stable controllable reconfigurability by using conformation-controlled base stacking. Exquisite control over DOJ's conformation and transformation is realized programming stacking bonds (quasi-blunt-ends) within induce prescribed...

We report a theory for biphasic ionic current signals during DNA and nanoparticle translocation through solid-state nanopore that produces scaling results consistent with continuum simulations, molecular dynamics experiments.

We report the first analytical theory on amplitude of resistive signals during molecular translocation through charged solid-state nanopores with variable cross-sectional area and piecewise-constant surface charge densities. By providing closed-form explicit algebraic expressions for concentration profiles inside nanopores, this allows prediction baseline without need numerical simulation electrokinetic phenomena. A transversely homogenized an asymptotic expansion weakly pores capture DC or...

By treating DNA as a vibrating nonlinear lattice, an activated kinetic theory for melting is developed to capture the breakage of hydrogen bonds and subsequent softening torsional bending vibration modes. With coarse-grained lattice model, we identify key mode with GHz frequency that replaces modes dominant out-of-phase phonon at transition state. associating its modulus universal in-phase equilibrium, can hence estimate entropic change in from near-equilibrium all-atom simulations. This...

Diffusion-mediated binding of molecules under the influence discrete spatially confining potentials is a commonly encountered scenario in systems subjected to explicit fields or implicit arising from tethering restraints. Here, we derive analytical expressions for mean time two random walkers geometrically confined by means harmonic one- and two-dimensional systems, which show excellent agreement with Brownian dynamics simulations. As demonstration its utility, use this theory maximize...

Abstract Solid-state nanopores allow high-throughput single-molecule detection but identifying and even registering all translocating small molecules remain key challenges due to their high translocation speeds. We show here the same electric field that drives into pore can selectively pin delay transport. A thin high-permittivity dielectric coating on slender bullet-shaped polymer permits leakage at tip produce a voltage-dependent surface upper periphery of reversibly edge-pin entering...

Abstract Microtubules in cells consist of functionally diverse subpopulations carrying distinct post-translational modifications (PTMs). Akin to the histone code, tubulin code regulates a myriad microtubule functions ranging from intracellular transport chromosome segregation. Yet, how individual PTMs only occur on subsets microtubules contribute specialization is not well understood. In particular, detyrosination, which removal C-terminal tyrosine α-tubulin subunits, marks stable population...

ABSTRACT Biomolecular nanotechnology has helped emulate basic robotic capabilities such as defined motion, sensing, and actuation in synthetic nanoscale systems. DNA origami is an attractive approach for nanorobotics, it enables creation of devices with complex geometry, programmed rapid actuation, force application, various kinds sensing modalities. Advanced functions like feedback control, autonomy, or routines also require the ability to transmit signals among sub-components. Prior work...