Infection with human papillomaviruses (HPVs) is one of the most common sexually transmitted infections and can lead to development head neck, skin, anogenital cancer, including cervical which represents world's significant health problems. In this study, we analyze G-rich regions in all known HPV genomes order evaluate their potential fold into G-quadruplex structure. Interestingly, loci fulfilling criteria for formation were found only 8 types HPV. Nevertheless, viral G-quadruplexes 7...

The present study expands the notion that simple oligonucleotide sequence d(TG(4)T) in solution forms a tetramolecular G-quadruplex having parallel orientation of four strands and G-quartets with all residues anti orientation. NMR experiments have revealed equilibrium two monomeric ratio between 85:15 70:30 presence K(+), Na(+), (15)NH(4)(+) ions. major form consists G-quartets, whereas minor exhibits an additional T-quartet at 5' end. An analogous U end adopts dimeric structure units K(+)...

We herein report on the formation and high-resolution NMR solution-state structure determination of a G-quadruplex adopted by d[G 3 ATG ACACAG 4 ACG ] comprised four G-tracts with third one consisting guanines that are intervened non-G streches different lengths. A single intramolecular antiparallel (3+1) exhibits three stacked G-quartets connected propeller, diagonal edgewise loops The propeller well structured, whereas longer loop is more flexible. To best our knowledge, this first where...

Abstract The G 4 C 2 hexanucleotide repeat expansion mutation (HREM) in C9ORF72 , represents the most common associated with amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration (FTLD). Three main disease mechanisms have been proposed to date: haploinsufficiency, RNA toxicity accumulation of dipeptide proteins. Pure GC content HREM potentially enables formation various non-B DNA structures such as G-quadruplexes i-motifs. These are act promoters regulatory elements...

NMR study has shown that DNA oligonucleotide d(G3T4G4) adopts an asymmetric bimolecular G-quadruplex structure in solution. The of d(G3T4G4)2 is composed three G-quartets, overhanging G11 residue and G3, which part the loop. Unique structural feature fold orientation two loops. Thymidine residues T4-T7 form a diagonal loop, whereas T15-T18 edge type core consists stacked G-quartets with syn-anti-anti-anti alternation dG one G-quartet syn-syn-anti-anti alternation. Another unusual leap...

Cocrystallisation of sildenafil with acetylsalicylic acid yields a 1 : sildenafil–acetylsalicylic-acid cocrystal, as well cocrystal salt involving cation, acetylsalicylate and salicylic acid. The acetylsalicylic-acid-based exhibits an enhanced intrinsic dissolution rate, compared to citrate (the active ingredient Viagra®).

Abstract A four‐repeat human telomere DNA sequence without the 3′‐end guanine, d[TAGGG(TTAGGG) 2 TTAGG] ( htel1‐ΔG23 ) has been found to adopt two distinct G‐quartet antiparallel basket‐type G‐quadruplexes, TD and KDH + in presence of KCl. NMR, CD, UV spectroscopy have demonstrated that topology form is distinctive with unique protonated T18⋅A20 ⋅G5 base triple other capping structural elements provide novel insight into polymorphism heterogeneity G‐quadruplexes general. Specific stacking...

<italic>In situ</italic> Raman monitoring revealed the first cocrystal as an intermediate in a mechanochemical organic reaction.

Abstract I-motifs are non-canonical nucleic acids structures characterized by intercalated H-bonds between hemi-protonated cytosines. Evidence on the involvement of i-motif in regulation cellular processes human cells has been consistently growing recent years. However, i-motifs within non-human genomes have never investigated. Here, we report characterization long terminal repeat (LTR) promoter HIV-1 proviral genome. Biophysical and biochemical analysis revealed formation a predominant with...

Tannin-based foams have gained attention as a potential bio-based alternative to conventional synthetic foams. Traditionally, namely condensed tannins (CT) been used, leaving the of hydrolysable (HT) largely unexplored. This study compared performance chestnut and quebracho in tannin-protein-based at different tannin ratios. Using soy protein isolate (SPI) hexamine under acidic conditions, series were produced through mechanical foaming method analyzed for cell structure, compression...

Background: In this study, two chalcone analogs were synthesized through in silico and experimental methods, their potential to inhibit the lipoxygenase enzyme, which plays a role inflammation pathway, was assessed. Specifically, study is continuation of previous research derivatives characterized. Objectives/Methods: current work, we present re-synthesis chalcones, with focus on docking studies, NMR analysis, dynamic simulations. The structure each elucidated combination Nuclear Magnetic...

Chloroplast thylakoid membranes contain virtually all components of the energy-converting photosynthetic machinery. Their energized state, driving ATP synthesis, is enabled by bilayer organization membrane. However, their most abundant lipid species a non-bilayer-forming lipid, monogalactosyl-diacylglycerol; role polymorphism in these poorly understood. Earlier 31P-NMR experiments revealed coexistence and non-bilayer, isotropic phase spinach thylakoids. Packing molecules, tested fluorescence...

Abstract A new folding intermediate of Oxytricha nova telomeric Oxy‐1.5 G‐quadruplex was characterized in aqueous solution using NMR spectroscopy, native gel electrophoresis, thermal differential spectra (TDS), CD and scanning calorimetry (DSC). experiments have revealed that this (i‐Oxy‐1.5) exists two symmetric bimolecular forms which all guanine bases are involved GG N1‐carbonyl base pairs. Kinetic analysis K + ‐induced structural transitions shows from i‐Oxy‐1.5 is much faster proceeds...

Abstract The role of non-bilayer lipids and non-lamellar lipid phases in biological membranes is an enigmatic problem membrane biology. Non-bilayer are present large amounts all membranes; energy-converting they constitute about half their total content—yet functional state a bilayer. In vitro experiments revealed that the functioning water-soluble violaxanthin de-epoxidase (VDE) enzyme plant thylakoids requires presence phase. 31 P-NMR spectroscopy has provided evidence on polymorphism...

Abstract In a growing context of green and circular economy, gaining knowledge the composition every crop is crucial, as this will allow for their full exploitation. Cherry ( Prunus avium L . ) widespread tree particular interest its fruits valuable timber. Its wood rich in extractives characterization to consider other applications feedstock. study, chipped cherry was extracted chemically analysed determine total phenolic content, condensed tannin, antioxidant capacity, polysaccharide...

This study examined the potential of hydrolysable tannin in comparison to condensed tannins for production furanic foams. The results indicate that chestnut presents lower reactivity and requires a stronger acid polymerization. Additionally, foamability density were found be dependent on both surfactant concentration type, allowing densities mimosa thermal conductivities chestnut-based Mimosa was have highest compression strength, followed by quebracho chestnut, promising conductivity around...

Fluconazole cocrystals were prepared with the intention to modify physicochemical properties of antifungal agent. The well-known COOH···Narom heterosynthon was considered key element in design strategy. Cocrystals fluconazole maleic, fumaric, and glutaric acid identified solution evaporation experiments pharmaceutically acceptable dicarboxylic acids their crystal structures are presented. Solid-state NMR as an alternative technique for providing structural information offered additional...

The effect of metal ions on the excited states guanine nanostructures, short d(TG4T)4 quadruplexes and long G4-wires, are studied by fluorescence spectroscopy. steady-state emission spectra show that both systems exhibit a strong cation effect. Fluorescence decays anisotropy decays, recorded from femtosecond to nanosecond time-scale, reveal following picture. In presence K+, arises mainly delocalized ππ* (excitons), whose decay spans several decades times. contrast, in Na+ is dominated...

Nuclear magnetic resonance study of G-quadruplex structures formed by d(TG 3 T) and its modified analogs containing a 5′-5′ or 3′-3′ inversion polarity sites, namely d(3′TG5′-5′G 2 T3′), d(3′T5′-5′G T3′) d(5′TG3′-3′G T5') demonstrates formation with tetrameric topology distinct cation-binding preferences. All oligonucleotides are able to form quadruplex two binding although the also form, in variable amounts, only one bound cation. The inter-quartet cavities at sites bind ammonium ions less...

This work presents a successful application of recently reported supramolecular strategy for stabilization metastable tautomers in cocrystals to monocomponent, non-heterocyclic, tautomeric solids. Quantum-chemical computations and solution studies show that the investigated Schiff base molecule, derived from 3-methoxysalicylaldehyde 2-amino-3-hydroxypyridine (ap), is far more stable as enol tautomer. In solid state, however, all three obtained polymorphic forms it exists solely keto...

Abstract A possible engineering of materials with diverse bio- and nano-applications relies on robust self-assembly oligonucleotides. Bottom-up approach utilizing guanine-rich DNA oligonucleotides can lead to formation G-wires, nanostructures consisting continuous stacks G-quartets. However, G-wire structure process remain poorly understood, although they are crucial for optimizing properties needed specific applications. Herein, we use nuclear magnetic resonance get insights at molecular...

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The thrombin binding aptamer, 15-mer oligonucleotide d[G2T2G2TGTG2T2G2], was folded into the well known antiparallel unimolecular G-quadruplex in presence of 15NH4+ ions. Although formed is thermodynamically less stable than K+ ions, loop conformations and folding topology are same. On other hand, titration Na+ ions an aqueous solution TBA resulted formation one major several minor species G-quadruplexes. Solution-state NMR used to localize between two G-quartets within core structure,...

G-wires—long, continuous G-quadruplexes—are a promising element for use in nanotechnology, particularly molecular electronics. The pairing of G-quadruplex structures through complementary GC-termini was tested. We designed four analogous G-quadruplex-forming oligonucleotides with the same core sequence d(GGTG4TGG) and different number position GC-ends. used circular dichroism to determine topology relative orientation G-quadruplexes, 1H NMR identify Watson–Crick base pairing, dynamic light...