A5059682321- Listeria monocytogenes in Food Safety
- Food Safety and Hygiene
- Salmonella and Campylobacter epidemiology
- Essential Oils and Antimicrobial Activity
- Microbial Inactivation Methods
- Parasitic Infections and Diagnostics
- Viral gastroenteritis research and epidemiology
- Identification and Quantification in Food
- Amoebic Infections and Treatments
- Clostridium difficile and Clostridium perfringens research
- Spectroscopy and Chemometric Analyses
- Biosensors and Analytical Detection
- Bacillus and Francisella bacterial research
- Microbial infections and disease research
- Probiotics and Fermented Foods
- Milk Quality and Mastitis in Dairy Cows
- Travel-related health issues
- Antimicrobial Resistance in Staphylococcus
- Parasite Biology and Host Interactions
- Medical Device Sterilization and Disinfection
- Plant Pathogenic Bacteria Studies
- Bacterial Identification and Susceptibility Testing
- Pediatric health and respiratory diseases
- Meat and Animal Product Quality
- Mycobacterium research and diagnosis
UK Health Security Agency
2021-2024
Public Health England
2013-2021
University of Liverpool
2016-2019
Special Olympics
2012
John Radcliffe Hospital
2009
Health Protection Agency
2005
CHU Dinant Godinne UCL Namur
2004
Wildfowl & Wetlands Trust
2004
University of Aberdeen
2004
University Hospital of Wales
2002
Cryptosporidium wrairi was isolated from guinea pigs during a spontaneous outbreak of cryptosporidiosis. Despite the morphological and antigenic similarities to C. parvum, displayed different host range site infection may represent separate species or sub-species. We used polymerase chain reaction clone two distinct 550 bp-long DNA fragments, Wc-I Wc-II, gene encoding oocyst wall protein (COWP) wrairi, which showed 98% identity parvum homologue. Within Wc-I, polymorphic Rsal restriction...
Cryptosporidium present in 1,705 fecal samples from humans and 105 livestock animals were analyzed by PCR-restriction fragment length polymorphism of the oocyst wall protein. Overall, genotype 1 (human exclusive type) was detected 37.8% humans, 2 (broad host range) 61.5%, a third designated 3 (Cryptosporidium meleagridis) 0.3%, both genotypes recovered 0.4%. All yielded 2. Among 469 patients infected during eight drinking water-related outbreaks, five outbreaks predominantly due to 1, three...
To study trends in human listeriosis and determine possible sources of infection.Descriptive analysis laboratory reports together with a survey subtypes Listeria monocytogenes isolated from patients foodstuffs an interview to obtain food histories.United Kingdom Republic Ireland 1985 1990.There was near doubling the incidence England, Wales, Northern between mid-1989 followed by sharp decline. The upsurge cases caused largely two strains L monocytogenes, which accounted for 30-54% annual...
Clinical information was collected on 722 cases of Listeria monocytogenes infections in humans occurring Britain between 1967 and 1985: 248 (34%) were associated with pregnancy (maternal, foetal, neonatal), comprised 9 (4%) maternal bacteraemia without infection the foetus; 42 (19%) intra-uterine deaths; 118 (54%) neonatal diagnosed within 2 days post-partum; 50 (23%) as ill after post-partum. An overall mortality 50% recorded. The unassociated are described elsewhere (see accompanying paper).
The 2006 Community Summary Report from the European Food Safety Authority (EFSA) and Centre for Disease Prevention Control (ECDC) was published recently with latest trends figures on occurrence of zoonotic infections agents, antimicrobial resistance foodborne outbreaks in then 25 Union (EU) Member States five non-EU countries. This article seeks to expand further upon reports human listeriosis (Listeria monocytogenes infections) changes epidemiology this disease, order inform important...
Non-typhoidal Salmonella enterica is a common cause of diarrhoeal disease; in humans, consumption contaminated poultry meat believed to be major source. Brazil the world’s largest exporter chicken globally, and previous studies have indicated introduction serovars through imported food products from Brazil. Here we provide an in-depth genomic characterisation evolutionary analysis investigate most prevalent antimicrobial resistance (AMR) Brazilian chickens assess impact public health with S...
ABSTRACT Samples of whole feces in which Cryptosporidium oocysts were recognized by hospital laboratories collected from 218 patients with diarrhea. All samples reexamined light microscopy, and detected 211 samples. A simple rapid procedure for the extraction DNA was developed, this used to amplify fragments outer wall protein (COWP), thrombospondin-related adhesive C1 (TRAP-C1), 18S rRNA genes PCR. For seven not microscopy failed be amplified three PCR procedures. Among “positive”...
Clinical information was collected on 722 cases of Listeria monocytogenes infections in humans occurring Britain between 1967 and 1985. This series comprised 34% (248 cases) associated with pregnancy 66% (474 non-pregnant adults juveniles. The not comprised: 76% patients severe underlying illness (of which had central nervous system infections, 42% bacteraemia without involvement the system); 21% previously healthy individuals whom 18% meningitis); 3% or system. Cases occurred most often...
The genetic basis of tetracycline resistance was studied in 25 clinical isolates Listeria monocytogenes. Resistance to associated with minocycline and due the presence tet(M) gene 24 strains. Association int-Tn, encoding protein required for movements Tn1545-like conjugative transposons, found all Cotransfer int-Tn among L. monocytogenes cells from Enterococcus faecalis detected 7 12 strains at frequencies similar those obtained prototype element Tn1545. tet(L), second most prevalent...
We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by unnested 17.6% COWP procedure. The gene was amplified from 2,128 samples: 71 livestock animals 2,057 humans. This series included 706 cases seven drinking water-associated outbreaks 51 five swimming pool-associated outbreaks, as well 1,300 sporadic cases.
Within the genus Listeria, Listeria monocytogenes is major pathogenic species. In humans disease from this bacterium (listeriosis) generally presents as an extremely severe systemic infection, although subclinical infection may also occur mild gastrointestinal or influenza-like illness. It believed that consumption of contaminated food principal route transmission. Epidemiological data indicate foodborne listeriosis can have variable incubation period (1->90 days), and attack rate for...
Strains of Listeria monocytogenes from 475 cases human listeriosis collected during 1967-1984, belonged to one three serogroups (1/2, 3 or 4). They were phage typed with a set 28 phages investigate aspects the epidemiology listeriosis. Three patients each had two episodes listeriosis, months 2 years apart, strains same serogroup and indistinguishable by typing. Ten possible cross-infection between pairs neonates in hospital occurred; first baby was ill at within 1 day birth, second became...