A5008286093- Ocular Surface and Contact Lens
- SARS-CoV-2 and COVID-19 Research
- Corneal Surgery and Treatments
- COVID-19 Clinical Research Studies
- Allergic Rhinitis and Sensitization
- Ocular Infections and Treatments
- Glaucoma and retinal disorders
- Infectious Diseases and Mycology
- Microbial infections and disease research
- COVID-19 epidemiological studies
- Eosinophilic Disorders and Syndromes
- Advanced Drug Delivery Systems
- Inflammasome and immune disorders
- Immune responses and vaccinations
- interferon and immune responses
- Mast cells and histamine
- Advanced Glycation End Products research
- Yersinia bacterium, plague, ectoparasites research
- Immunotherapy and Immune Responses
- Bacterial Identification and Susceptibility Testing
- Carbon Nanotubes in Composites
- Herpesvirus Infections and Treatments
- Immune Response and Inflammation
- Intraocular Surgery and Lenses
- Influenza Virus Research Studies
University of Naples Federico II
2007-2025
Technobiochip (Italy)
2020
Aims/Purpose: To evaluate the efficacy of correlated Scanning Electron Microscopy (SEM)/Optical (OM) analysis conjunctival scrapings in diagnosing infectious keratitis with prior negative culture tests or ineffective broad‐spectrum antibiotic therapy. Methods: 102 eyes 87 patients (45M, 42F) affected by were evaluated cytological examination superior tarsal conjunctiva OM (Nikon Eclipse 50i) and SEM (JEOL), after 72 h wash‐out. Inclusion criteria: age > 18 years, corneal epithelial...
Aims/Purpose: The aim of the study is to evaluate with SEM residence time and ultrastructural effects on ocular surface cells in patients suffering from dry eye disease (DED) treated a multiple‐acting tear substitute based 0.15% cross‐linked HA, cationic liposomes 3% trehalose (Trimix®, Offhealth SpA, Firenze, Italy). Methods: We examined 10 eyes previous clininal diagnosis DED (Schirmer test 1 < mm, TBUT s, OSDI > 13). Exclusion criteria: topical or systemic therapies 30 days;...
Conjunctival microvilli, small protrusions of the plasma membrane on top surface epithelial cells, play a key role in keeping eye balanced. They help to steady tear film and keep epithelium moist. New advances scanning electron microscopy (SEM) when used with impression cytology (IC), have improved our ability study these tiny structures. SEM provides detailed 3D images conjunctival microvilli making it easier spot early changes structure linked problems like dry syndrome, Sjögren's...
ESKAPE pathogens are responsible for complicated nosocomial infections worldwide and usually resistant to commonly used antibiotics in clinical settings. Among these bacteria, vancomycin-resistant Enterococcus faecium methicillin-resistant Staphylococcus aureus the two most important Gram-positive which alternative treatments preventions urgently needed. We previously designed a multi-presenting antigen, embedding main epitope displayed by AdcA protein of E. faecium, that conferred...
65 consecutive patients with presumed microbial keratitis and negative culture tests for bacteria fungi obtained by corneal curettage were evaluated. All had undergone local broad spectrum antibiotic therapy at least 5 days no clinical improvement. After 48–72 h of wash-out they underwent scraping the superior tarsal conjunctiva cytological examination cellular morphology in Scanning Electron Microscopy (SEM). The presence pathogenic microorganisms was detected this method 62 these patients,...
The authors performed an ex vivo and in evaluation of the ultrastructural effects on conjunctival epithelial cells a new multiple-action tear substitute containing cross-linked hyaluronic acid, lipids trehalose (Trimix®), using scanning electron microscopy (SEM) with impression cytology. study highlights persistence distribution product at 5 60 min monolayer increase microvilli density evaluation. In examination was conducted three subjects different grades ocular surface inflammation,...
The common approach of the diagnosis Alzheimer’s Disease (AD) is made with an analysis cerebrospinal fluid or study retinal fundus and plaques formation through optical corneal tomography (OCT), more simply a camera. Tears widely discussed in literature as essential method to describe molecular biochemical alterations different diseases. aim our was identification immunocytochemistry Amyloid Beta-42 tears from patients without familiarity for Alzheimer Disease, order make earlier accessible...
Nasal polyposis is characterized by benign, non-cancerous and painless growths originating in the tissue of nasal cavities paranasal sinuses. Polyps arise from chronic inflammation due to asthma, recurrent infections, allergies, drug sensitivity or immune disorders. They can obstruct thus cause respiratory problems, a reduction sense smell susceptibility infections. Furthermore, polyps recur. Hence importance using valid diagnostic methods. In this work, investigation carried out scanning...
Human perpetual exposure to several pollutants such as smoke, radiation, and toxic chemical substances, causes an excessive production of reactive oxygen species (ROS), that leads the onset neurodegeneration diseases. This knowledge has highlighted importance frequent monitoring people health with innovative biosensors able detect pathological conditions at initial stage. Indeed, it is mainly stated neurodegenerative diseases can be effectively treated only if diagnosed very early. In this...
SARS-CoV-2 is a new pandemic infection that affects at the beginning upper respiratory system, and, successively, all organisms, due to cytokine storm, with serious consequences can reach death. The aim of this work was observation nasal mucosa enrolled 60 patients, resulting negative for two weeks molecular swab SARS-CoV-2, versus control group. Rhino-fibroscopy and cytology were performed both investigated groups. samples showed occurrence plasmablastic lymphocytes Downey II type. former...
Abstract Introduction : The common approaches at the diagnosis of Alzheimer Disease(AD) is made with an analysis cerebrospinal fluid or last techniques use study retinal fundus and plaques formation, through OCT more simply a camera. Tears’ widely discussed in literature as essential method to describe molecular biochemical alterations different diseases. aim our was identification Immunocytochemistry Amyloid Beta-42 tears from patients without familiarity for disease, order make earlier...
Purpose: This work is aimed at demonstrating that scraping cytology and scanning electron microscopy can successfully assist in the diagnosis of nontuberculous mycobacteria infection.For this purpose, we report use both these techniques cornel ulcer a previously healthy young man.Methods: Cytological samples were achieved by technique on mucosa, sub palpebral temporal area eye tarsal conjunctiva.The obtained sample was affi xed to sanded rectangular slide, stained with Pappenheim method,...
The Hypothesis born on a simple clinical data noted by some Chinese Reserchers during the starting point of epidemic began in dicember 2019, for novel member human coronavirus, officially named as SARS‐CoV‐2 (severe acute respiratory syndrome coronavirus 2) International Committee Taxonomy Viruses (ICTV) is new strain RNA viruses that has not been previously identified humans [1].