A5100605878- Click Chemistry and Applications
- Estrogen and related hormone effects
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Hepatitis B Virus Studies
- Prostate Cancer Diagnosis and Treatment
- Computational Drug Discovery Methods
- Genetics, Bioinformatics, and Biomedical Research
- Peptidase Inhibition and Analysis
- Cervical Cancer and HPV Research
- HER2/EGFR in Cancer Research
- Neuroendocrine Tumor Research Advances
- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- Liver Disease Diagnosis and Treatment
- Advanced Biosensing Techniques and Applications
- Lysosomal Storage Disorders Research
- Hepatitis C virus research
- Gastric Cancer Management and Outcomes
- Surgical Simulation and Training
- Pancreatic function and diabetes
- Immune cells in cancer
- Genetic Syndromes and Imprinting
- Cancer, Hypoxia, and Metabolism
Enzo Life Sciences (United States)
2022-2024
Eisai (United States)
2009-2024
WuXi AppTec (China)
2024
Duke University
2024
University of Virginia Cancer Center
2021-2023
University of Virginia
2021-2023
China Medical University Hospital
2010-2023
China Medical University
2010-2023
Virginia Cancer Institute
2023
Public Health Ontario
2019-2021
Abstract Human papillomavirus (HPV) is a significant contributor to the global cancer burden, and its carcinogenic activity facilitated in part by HPV early protein 6 (E6), which interacts with E3-ligase E6AP, also known as UBE3A, promote degradation of tumor suppressor, p53. In this study, we present single-particle cryoEM structure full-length E6AP complex HPV16 E6 (16E6) p53, determined at resolution ~3.3 Å. Our reveals extensive protein-protein interactions between 16E6 explaining their...
Abstract Paxillin (PXN) gene mutations are associated with lung adenocarcinoma progression and PXN is known to be a target of microRNA-218 (miR-218). On this basis, we hypothesized that overexpression via miR-218 suppression may promote tumor metastasis predict survival relapse in non–small cell cancer (NSCLC). Expression 124 surgically resected tumors were evaluated by real-time PCR immunohistochemical analysis. The prognostic value expression on overall (OS) relapse-free (RFS) was analyzed...
Mutations in estrogen receptor alpha (ERα) that confer resistance to existing classes of endocrine therapies are detected up 30% patients who have relapsed during treatments. Because a significant proportion therapy-resistant breast cancer metastases continue be dependent on ERα signaling, there remains critical need develop the next generation antagonists can overcome aberrant activity. Through our drug-discovery efforts, we identified H3B-5942, which covalently inactivates both wild-type...
In this report the authors describe use of hemostatic agent recombinant activated factor VII (rFVIIa) in perioperative treatment hemorrhages central nervous system that are associated with warfarin therapy.Two patients sustained within spinal canal, and other two had acute intracranial subdural hematomas. All normal platelet counts, partial thromboplastin times, fibrinogen levels, all received fresh frozen plasma conjunction rFVIIa. The initial international normalized ratios (INRs) ranged...
Abstract Inactivation of p53 by human papillomavirus 16/18 E6 plays a crucial role in cervical tumorigenesis. To investigate the involvement HPV16/18 lung tumorigenesis, association between HPV16 or HPV18 and protein expression 122 tumors was evaluated immunohistochemistry, data showed that correlated inversely with expression, which further confirmed tissue situ immunostaining. Real-time reverse transcription-PCR analysis indicated E6-positive had lower p21WAF1/CIP1 mdm2 mRNA levels than...
DDX3 alteration has been shown to participate in hepatocellular tumorigenesis via p21(WAF1/CIP1) (p21) deregulation. We observed that and p21 expression lung tumors was negatively associated with E6 expression. Therefore, the aim of this study clarify whether deregulation by an E6-inactivated p53 pathway would enhance tumor progression HPV-associated cancers.Real-time PCR, luciferase assays, immunoprecipitation, chromatin immunoprecipitation (ChIP) were performed determine regulated...
// Po-Chun Chen 1,2 , Hsu-Chen Cheng 3 John Wang 4 Shin-Wei 5 Huai-Ching Tai 6,7 Chiao-Wen Lin 8,9 and Chih-Hsin Tang 1,10,11 1 Graduate Institute of Basic Medical Science, China University, Taichung, Taiwan 2 Department Research, Chung Shan University Hospital, Life Sciences, National Hsing Pathology, Taichung Veterans General Medicine, Mackay College, New Taipei City, 6 Urology, Taipei, 7 Clinical 8 Oral 9 Dentistry, 10 Pharmacology, School 11 Biotechnology, College Health Asia...
Human papillomavirus (HPV) infections account for nearly all cervical cancer cases, which is the fourth most common in women worldwide. High-risk variants, including HPV16, drive tumorigenesis part by promoting degradation of tumor suppressor p53. This mediated HPV early protein 6 (E6), recruits E3 ubiquitin ligase E6AP and redirects its activity towards ubiquitinating Targeting interaction interface between E6 a promising modality to mitigate HPV-mediated In this study, we designed covalent...
Abstract Purpose: Fas ligand (FasL) −844T/C polymorphism (rs763110) has a demonstrated association with lung cancer risk. FasL −844CC higher expression been suggested to contribute tumor progression via immune escape. However, the impact of on clinical outcome non–small cell (NSCLC) remains be identified. Experimental Design: A total 385 adjacent normal tissues from patients NSCLC were collected determine by PCR-based restriction fragment length polymorphism. mRNA and protein in tumors...
To analyze the learning curve for cancer control from an initial 250 cases (Group I) and subsequent II) of robotic-assisted laparoscopic radical prostatectomy (RALP) performed by a single surgeon. Five hundred consecutive patients with clinically localized prostate received RALP were evaluated. Surgical parameters perioperative complications compared between groups. Positive surgical margin (PSM) biochemical recurrence (BCR) assessed as outcomes. Patients in Group II had significantly more...
Metabolic risk factors, such as obesity, fatty liver, high lipidemia, and diabetes mellitus are associated with increased for nonviral hepatocellular carcinoma (HCC); however, few HCC studies have stratified patients according to underlying etiologies. From 2005 2011, 3,843 were recruited into the Taiwan Liver Cancer Network. Of these patients, 411 (10.69%) who negative hepatitis B virus (HBV), surface antigen, HBV DNA, anti‐hepatitis C (HCV) antibody classified non‐HBV non‐HCV (NBNC)‐HCC....
We report a unique case of combined primary neuroendocrine carcinoma (NEC) and hepatocellular (HCC) the liver in 65-year-old male patient. The patient underwent segmental resection regional lymph node dissection for tumor mass that measured 7.5 cm diameter right lobe, with lymphadenopathy. Histologically, hepatic was composed predominantly small-cell NEC, but admixed small island moderately differentiated HCC. speculate NEC originated from poorly clone an HCC differentiation, this now at end...
Objectives We analyze and discuss the importance of intrarenal color duplex ultrasonography (CDUS) in diagnosis differential vascular complications renal transplants. Methods retrospectively reviewed results CDUS, especially 102 consecutive inpatients with transplants from January 1, 2000, to December 31, 2006. Correlations between CDUS magnetic resonance angiography, digital subtraction surgical findings, clinical diagnoses were studied. Results Abnormal findings on clearly represent...
Hepatocellular carcinoma (HCC) is the leading cancer death in Taiwan. Chronic viral hepatitis infections have long been considered as most important risk factors for HCC The previously published reports were either carried out by individual investigators with small patient numbers or large endemic studies limited marker data. Through collaboration 5 medical centers across Taiwan, Taiwan liver network (TLCN) was established 2005. All participating followed a standard protocol to recruit...
Diabetic nephropathy is characterized as the progressive development of renal insufficiency in a setting hyperglycemia. Previous studies indicate that reactive oxygen species (ROS) play an important role high glucose-induced injury. Cilostazol was reported to lower production superoxide significantly situ. We hypothesized cilostazol administration streptozotocin-induced diabetic rats exerts effects via improving oxidative stress. Male Sprague–Dawley were fed with (5 mg/kg or 25 mg/kg) for 12...
Transcutaneous pressure of oxygen (PtcO2) correlates with arterial (PaO2) in nonshock states, but shock PtcO2 approximates cardiac output no response to increasing fraction inspired (FiO2) and PaO2. An incremental change more than 21 mmHg an FiO2 1.0 (identified as the challenge test [OCT]) implies adequate tissue perfusion, lack has been associated mortality. Patients severe sepsis septic requiring pulmonary artery catheters were randomized two groups: delivery (DO2) group was treated a DO2...
Abstract α‐Klotho, an aging‐related protein found in the kidney, parathyroid gland, and choroid plexus, acts as essential co‐receptor with fibroblast growth factor 23 receptor complex to regulate serum phosphate vitamin D levels. Decreased levels of α‐Klotho are a hallmark age‐associated diseases. Detecting or labeling biological milieu has long been challenge, however, hampering understanding its role. Here, we developed branched peptides by single‐shot parallel automated fast‐flow...
The aim of this study was to assess the relationship between intrarenal Doppler parameters and histopathologic changes shown on kidney biopsy in renal transplant dysfunction.We retrospectively reviewed records 113 patients (61 men 52 women; age range, 22-76 years; mean ± SD, 50.9 12.7 years) who underwent both transplanted sonography from May 1, 2007, 31, 2009. interlobar arteries, including peak systolic velocity (PSV), end-diastolic (EDV), resistive index (RI), were compared with findings....