A5100349278- Microbial bioremediation and biosurfactants
- Pesticide and Herbicide Environmental Studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Fuel Cells and Bioremediation
- Microbial Community Ecology and Physiology
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- bioluminescence and chemiluminescence research
- Advanced Measurement and Detection Methods
- Plant Disease Resistance and Genetics
- Analytical Chemistry and Chromatography
- Plant-Microbe Interactions and Immunity
- Pharmacogenetics and Drug Metabolism
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Microbial metabolism and enzyme function
- Enzyme Production and Characterization
- Image and Object Detection Techniques
- Environmental Chemistry and Analysis
- Structural Engineering and Vibration Analysis
- Insect symbiosis and bacterial influences
- Enzyme-mediated dye degradation
- Constructed Wetlands for Wastewater Treatment
- Protist diversity and phylogeny
- Phytochemistry and Bioactivity Studies
Nanjing Agricultural University
2019-2024
Ministry of Agriculture and Rural Affairs
2022-2024
Ningbo University
2024
Chang'an University
2020
Jilin University
2020
Dalian University of Technology
2009
China Tourism Academy
2008
Abstract Strains Rhodococcus qingshengii djl‐6 and jialingiae djl‐6‐2 both harbour the typical carbendazim degradation pathway with hydrolysis of to 2‐aminobenzimidazole (2‐AB) as initial step. However, enzymes involved in this process are still unknown. In study, previous reported hydrolase MheI was found strain djl‐6, but not djl‐6‐2, then another CbmA obtained by a four‐step purification strategy from djl‐6‐2. classified member amidase signature superfamily conserved catalytic site...
Strobilurin fungicides are widely used in agricultural production due to their broad-spectrum and fungal mitochondrial inhibitory activities. However, massive application has restrained the growth of eukaryotic algae increased collateral damage freshwater systems, notably harmful cyanobacterial blooms (HCBs). In this study, a strobilurin fungicide-degrading strain, Hyphomicrobium sp. strain DY-1, was isolated characterized successfully. Moreover, novel esterase gene, strH, responsible for...
Although enzyme-encoding genes involved in the degradation of carbaryl have been reported Pseudomonas sp. strain XWY-1, no regulator has identified yet. In mcbABCDEF cluster responsible for upstream pathway (from to salicylate), mcbA gene is constitutively expressed, while mcbBCDEF induced by 1-naphthol, hydrolysis product McbA. this study, we McbG, a transcriptional activator cluster. McbG 315-amino-acid protein with molecular mass 35.7 kDa. It belongs LysR family regulators and shows...
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In this study, we isolated and characterized the bacterial strain Pseudomonas sp. BYT-1, which is capable of degrading pymetrozine using it as sole carbon source for growth. Strain BYT-1 could degrade 2.30 mM within 20 h under optimal conditions 30 °C pH 7.0. Investigation degradation pathway showed that was oxidatively hydrolyzed to 4-amino-6-methyl-4,5-dihydro-2 H-[1,2,4]triazin-3-one (AMDT) nicotinic acid (NA). The former accumulates end product in culture, whereas latter hydroxylated...
Degradation of the fungicide iprodione by Paenarthrobacter sp. strain YJN-5 is initiated via hydrolysis its N1 amide bond to form N-(3,5-dichlorophenyl)-2,4-dioxoimidazolidine. In this study, another iprodione-degrading strain, YJN-D, which harbours same metabolic pathway as was isolated and characterized. The genes that encode conserved catabolic were identified based on comparative analysis genomes two subsequent experimental validation. These include an amidase gene, ipaH (previously...
Carbaryl is a widely used carbamate pesticide in agriculture. The strain Rhizobium sp. X9 possesses the typical carbaryl degradation pathway which mineralized via 1-naphthol, salicylate, and gentisate. In this study, we cloned hydrolase gene cehA novel two-component 1-naphthol hydroxylase cehC1C2. CehA mediates hydrolysis to CehC1, an FMNH2 or FADH2-dependent monooxygenase belonging HpaB superfamily, hydroxylates presence of reduced nicotinamide-adenine dinucleotide (FMN)/flavin adenine...
Based on the structural characteristic, carbamate insecticides can be classified into oxime carbamates (methomyl, aldicarb, oxamyl, etc.) and N -methyl (carbaryl, carbofuran, isoprocarb, etc.). So far, research degradation of pesticides has mainly focused detoxification step hydrolysis their bond. Several genes, such as cehA , mcbA cahA mcd encoding enzymes have also been reported to involved in step. However, none these hydrolyze methomyl. In this study, a C-N hydrolase gene, ameH...
Carbaryl is the representative of carbamate insecticide. As an acetylcholinesterase inhibitor, it poses potential threat to humans and other non-target organisms. Agrobacterium sp. XWY-2, which could grow with carbaryl as sole carbon source, was isolated characterized. The carH gene, encoding a hydrolase, cloned from strain XWY-2 expressed in Escherichia coli BL21 (DE3). CarH able hydrolyze pesticides including carbaryl, carbofuran, isoprocarb, propoxur fenobucarb efficiently, while...
Owing to the extensive use of carbofuran over past 50 years, bacteria have evolved catabolic pathways mineralize this insecticide, which plays an important role in eliminating residue environment. In study, cfd gene cluster, responsible for catabolism phenol, was predicted by comparing sphingomonad genomes.
Summary Pymetrozine is a synthetic pesticide that can be utilized as the sole carbon source by Pseudomonas sp. strain BYT‐1. However, genes involved in degradation of pymetrozine remain unknown. We used transposon mutagenesis to create mutant unable hydrolyze pymetrozine. The interrupted gene pyzH , which was cloned self‐formed adaptor PCR. PyzH hydrolyzed C=N double bond produce 4‐amino‐6‐methyl‐4,5‐dihydro‐2 H ‐[1,2,4]triazin‐3‐one (AMDT) and nicotinaldehyde; latter inhibits activity....