A5052303363- Ovarian cancer diagnosis and treatment
- BRCA gene mutations in cancer
- Endometrial and Cervical Cancer Treatments
- CRISPR and Genetic Engineering
- Nutrition, Genetics, and Disease
- Transgenic Plants and Applications
- Animal Genetics and Reproduction
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Toxin Mechanisms and Immunotoxins
- Glioma Diagnosis and Treatment
- Brain Metastases and Treatment
- Computational Drug Discovery Methods
- Genetically Modified Organisms Research
- Gene expression and cancer classification
- Cancer-related cognitive impairment studies
- Genetics, Aging, and Longevity in Model Organisms
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Uterine Myomas and Treatments
- Gynecological conditions and treatments
- Statistical Methods in Clinical Trials
- Estrogen and related hormone effects
- Cytokine Signaling Pathways and Interactions
- Cervical Cancer and HPV Research
The Barbara Ann Karmanos Cancer Institute
2006-2025
Wayne State University
2002-2024
Brighton and Sussex Medical School
2004-2006
University of Sussex
2005-2006
Cancer Research UK
2005-2006
The neuropsychological performance of 85 women with early stage breast cancer scheduled for chemotherapy, 43 endocrine therapy and/or radiotherapy and 49 healthy control subjects was assessed at baseline (T1), postchemotherapy (or 6 months) (T2) 18 months (T3). Repeated measures analysis found no significant interactions or main effect group after controlling age intelligence. Using a calculation to examine an individual level, reliable decline on multiple tasks seen in 20% chemotherapy...
This study explores the role of titin, a giant muscle protein, in progression epithelial ovarian cancer (EOC). We examined titin levels tissues and sera from EOC patients across stages I-IV chemoresistant cells. Tissue samples underwent immunohistochemistry, serum were measured using ELISA. Quantitative real-time PCR analyzed mRNA cell lines, including chemosensitive, chemoresistant, normal Notably, elevated detected 90.9% stage I compared to only 14.3% III IV tissues. Serum consistently...
<p>(A) qPCR analysis of Wnt inhibitors Dkk1, Notum, and Axin2 in ID8 mouse ovarian cancer cells. Data are presented as mean +/- SEM (n=3). Ordinary one-way ANOVA with Dunnett’s multiple comparison test was used to calculate statistical significance; (B) Western blot validates upregulation upon loss BRCA2.</p>
<p>Chord diagrams of top 10 differentially regulated Pathways and involved DEGs in BRCA2mt vs HRwt</p>
<p>qPCR analysis for Wnt3A and Ctnnb1 in ID8 mouse ovarian cancer cells. Data are presented as mean +/- SEM (n=3). Ordinary one-way ANOVA was used to calculate statistical significance.</p>
<p>(A) DEGs in BRCA2null vs BRCA1null 4T1 mouse breast cancer cell line reported as log fold-change (logFC); red bars are upregulated and blue downregulated; (B) Perturbation analysis of Wnt signaling pathway comparing line; genes predicted to be downregulated.</p>
<p>Nuclear and cytoplasmic green fluorescence were measured in all cells from cultures represented Figure 3C using image J. Nuclear (left panel) (right quantifications are reported separately for each cell line. Statistical significance between control treated was not achieved.</p>
<p>Chord diagrams of top 10 differentially regulated Pathways and involved DEGs in BRCA1mt vs HRwt</p>
<p>Chord diagrams of top 10 differentially regulated Pathways and involved DEGs in BRCA2mt vs BRCA1mt</p>
16000 Background: Although the majority of women with EC are postmenopausal, 25% under age 50 years at time diagnosis. The aim this study is to describe demographic and survival data for presenting 35 or younger (YOY). Design: Patients a diagnosis were identified from Surveillance, Epidemiology, End Results (SEER) cancer database 1988 2003. Demographic pathologic collected. Chi-square t-tests used examine differences between older women, analyses performed using Kaplan Meier Cox regression...
Abstract The association of BRCA1 and BRCA2 mutations with increased risk for developing epithelial ovarian cancer is well established. However, the observed clinical differences, particularly improved therapy response patient survival in BRCA2-mutant patients, are unexplained. Our objective to identify molecular pathways that differentially regulated upon loss functions cancer. Transcriptomic pathway analyses comparing BRCA1-mutant, BRCA2-mutant, homologous recombination wild-type tumors...
<p>The noncanonical Wnt pathway is activated upon loss of <i>BRCA1</i>. <b>A,</b> Model the signaling pathway. <b>B,</b> ID8 mouse ovarian cancer cells were treated with 100 ng/mL Wnt3A followed by immunofluorescence for F-actin (green). DAPI staining shown in blue. Red arrows point to filaments. Images show both green and channels. <b>C,</b> Biological processes related filament microtubule polymerization are differentially regulated...
<p>Transcriptomic differences between patients with <i>BRCA1</i>mt, <i>BRCA2</i>mt, and HRwt ovarian cancer. RNA-seq was performed on (<i>n</i> = 375), <i>BRCA1</i>mt 16), <i>BRCA2</i>mt 15) <b>A,</b> Volcano plots showing the distribution of DEGs (<i>P</i> 0.05; FC > 1.5) in compared groups as indicated. <b>B,</b> Venn diagram each 163 genes unique to tumors. <b>C,</b>...
<div>Abstract<p>The association of <i>BRCA1</i> and <i>BRCA2</i> mutations with increased risk for developing epithelial ovarian cancer is well established. However, the observed clinical differences, particularly improved therapy response patient survival in <i>BRCA2</i>-mutant patients, are unexplained. Our objective to identify molecular pathways that differentially regulated upon loss functions cancer. Transcriptomic pathway analyses...
<p>(A) qPCR analysis of Wnt inhibitors Dkk1, Notum, and Axin2 in ID8 mouse ovarian cancer cells. Data are presented as mean +/- SEM (n=3). Ordinary one-way ANOVA with Dunnett’s multiple comparison test was used to calculate statistical significance; (B) Western blot validates upregulation upon loss BRCA2.</p>
<p>Chord diagrams of top 10 differentially regulated Pathways and involved DEGs in BRCA2mt vs BRCA1mt</p>
<p>Characterization of the Wnt signaling pathway in isogenic mouse ovarian cancer cells upon loss Brca1 or Brca2. Cellular fractionation was performed to separate cytoplasmic (<b>A</b>) and nuclear (<b>B</b>) fractions control no-treatment treated with 100 ng/mL Wnt3A for 8 hours; ILF3 Cox-IV were used as integrity controls fractions, respectively. <b>C,</b> Levels cellular location β-catenin determined by immunofluorescence (green). DAPI staining is...
<p>qPCR analysis for Wnt3A and Ctnnb1 in ID8 mouse ovarian cancer cells. Data are presented as mean +/- SEM (n=3). Ordinary one-way ANOVA was used to calculate statistical significance.</p>
<p>Decreased tumor growth kinetics and improved survival in mice bearing ID8<sup><i>Trp53−/−</i>;<i>Brca2−/−</i></sup> cells. ID8 mouse ovarian cancer cells were injected intraperitoneally C57BL/6 (<i>n</i> = 6). <b>A,</b> Abdominal width was used as surrogate for intraperitoneal growth; note the significant decrease Data are presented mean ± SEM. Two-way ANOVA with mixed-effect analysis to calculate statistical significance....
<p>Nuclear and cytoplasmic green fluorescence were measured in all cells from cultures represented Figure 3C using image J. Nuclear (left panel) (right quantifications are reported separately for each cell line. Statistical significance between control treated was not achieved.</p>