A5082930636- Pharmacogenetics and Drug Metabolism
- Microbial Metabolic Engineering and Bioproduction
- Computational Drug Discovery Methods
- Coronary Interventions and Diagnostics
- Cardiac Imaging and Diagnostics
- Drug Transport and Resistance Mechanisms
- Acute Myocardial Infarction Research
- Fungal and yeast genetics research
- Enzyme Catalysis and Immobilization
- Steroid Chemistry and Biochemistry
- Analytical Chemistry and Chromatography
- Cardiac Valve Diseases and Treatments
- Biochemical Acid Research Studies
- Chemical Reactions and Isotopes
- Electron Spin Resonance Studies
- Amino Acid Enzymes and Metabolism
- Protein Structure and Dynamics
- Bioinformatics and Genomic Networks
- Cardiovascular Effects of Exercise
- Metal-Catalyzed Oxygenation Mechanisms
- Eicosanoids and Hypertension Pharmacology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Coenzyme Q10 studies and effects
- Mass Spectrometry Techniques and Applications
- Chronic Disease Management Strategies
Centre National de la Recherche Scientifique
2009-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Université de Toulouse
2014-2025
Biotechnology Institute
2022-2025
Institut National des Sciences Appliquées de Toulouse
2015-2024
Hôpital Européen
2020
Assistance Publique – Hôpitaux de Paris
2020
Hôpital Européen Georges-Pompidou
2020
Centre de Génétique Moléculaire
1997-2018
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2013-2018
Two NADPH-cytochrome P450 reductase-encoding cDNAs were isolated from an <i>Arabidopsis</i> cDNA library by metabolic interference in a <i>Saccharomyces cerevisiae</i> mutant disrupted for its endogenous <i>cpr1</i>gene. <i>ATR1</i> encodes protein of 692 amino acids, while<i>ATR2</i> either 712-residue (ATR2-1), or 702-residue (ATR2-2) depending on the choice initiation codon. Comparative analysis ATR1 and ATR2-1 indicates 64% acid sequence identity absence conservation third base conserved...
The standard redox potential of ubiquinone in submitochondrial particles is measured by equilibration with the succinate/fumarate system to about 65 mV at pH 7, ratio, n = 2. dependence ‐60 mV/Δ pH. lower value for bound discussed comparison found isolated (104 112 mV, 7) determined ethanol‐HCl. In parallel experiments cytochrome b was 72.5mV 7. Δ E/Δ 0 < 6.8 and ‐ 60 ΔHp > 6.8.
Helianthus tuberosus cinnamate 4‐hydroxylase (CYP73 or CA4H), a member of the P 450 superfamily which catalyses first oxidative step phenylpropanoid pathway in higher plants by transforming into p ‐coumarate, was expressed yeast Saccharomyces cerevisiae . The PCR‐amplified CA4H open reading frame inserted pYeDP60 under transcriptional control galactose‐inducible artificial promoter. Engineered S. cerevisiæ strains producing human reductase normal overproduced amounts were transformed to...
OBJECTIVE: To decrease pre-hospital delay in patients with chest pain. DESIGN: Population based, prospective observational study. SETTING: A province of Switzerland 380000 inhabitants. SUBJECTS: All 1337 who presented pain to the emergency department Hôpital Cantonal Universitaire Geneva during 12 months a multimedia public campaign, and 1140 came similar symptoms before campaign started. MAIN OUTCOME MEASURES: Pre-hospital time number admitted hospital for acute myocardial infarction (AMI)...
Between September 1985 and April 1987, 110 consecutive patients who had successful coronary angioplasty were included in a randomised prospective controlled evaluation of the effects warfarin on restenosis. The (n = 56) control 54) groups not different terms age, sex, previous bypass surgery or balloon angioplasty, severity symptoms, frequency multivessel disease total occlusions. Warfarin was started day procedure dosage adjusted to maintain thromboplastin international normalised ratio...
Abstract Understanding protein structure requires studying its dynamics, which is critical to elucidating functional role. Biophysical techniques have revolutionized this field over time, providing remarkable insights into structure‐function relationships. Among these, Site‐Directed Spin Labelling (SDSL) combined with Electron Paramagnetic Resonance (EPR) a powerful method delivering structural data at the residue level, irrespective of size or environment. Traditional nitroxide labels...
Human liver P 450 NF25 (CYP3A4) had been previously expressed in Saccharomyces cerevisiae using the inducible GAL10‐CYC1 promoter and phosphoglycerate kinase gene terminator [Renaud, J. P., Cullin, C., Pompon, D., Beaune, P. Mansuy, D. (1990) Eur. Biochem. 194 , 889–896]. The use of an improved expression vector [Urban, C. Biochimie 72 463–472] increased amounts produced/culture medium by a factor five, yielding up to 10 nmol/l. availability recently developed host cells that simultaneously...
Microsomal epoxide hydrolase (mEH) belongs to the superfamily of alpha/beta-hydrolase fold enzymes. A catalytic triad in active centre enzyme hydrolyses substrate molecules a two-step reaction via intermediate formation an enzyme-substrate ester. Here we show that mEH is composed Asp226, Glu404 and His431. Replacing either these residues with non-functional amino acids results complete loss activity recombinantly expressed Saccharomyces cerevisiae. For His431 mutants, their structural...
The flavoprotein Cytochrome P450 reductase (CPR) is the unique electron pathway from NADPH to (CYPs). conformational dynamics of human CPR in solution, which involves transitions a "locked/closed" an "unlocked/open" state, crucial for transfer. To date, however, factors guiding these changes remain unknown. By Site-Directed Spin Labelling coupled Electron Paramagnetic Resonance spectroscopy, we have incorporated non-canonical amino acid onto flavin mononucleotide (FMN) and adenine...
For several years, acute coronary syndromes have been perceived as causing the most hospital admissions, and even mortality. The syndrome of unstable angina frequently progresses to myocardial infarction but its pathogenesis is poorly understood, prognosis determination still problematic. We tested hypothesis that measurement C-reactive protein in patients admitted for chest pain could be a marker syndromes.We studied 110 with suspected ischaemic heart disease, without elevated serum...
Human microsomal epoxide hydrolase and cytochrome P 450 ( 450) 1A1 were coexpressed in Saccharomyces cerevisiae from expression cassettes integrated respectively into the host chromosomal DNA on a multicopy plasmid strain already overexpressing yeast NADPH‐cytochrome reductase reductase). A styrene‐oxide‐hydrolase activity (2 nmol · min −1 mg protein ) 7‐ethoxyresorufin‐ O ‐deethylase (320 pmol characteristic of detected. The conversion benzo[a]pyrene (B[a]P) to B[a]P‐7,8‐dihydrodiol both...
NADPH cytochrome P450 oxidoreductase (CPR) is the obligatory electron supplier in sustaining activity of microsomal (CYP). The variant nature isoform specific proximal interface CYPs implies that CPR capable multiple degenerated interactions with for transfer, through different binding mechanisms, which are still not well understood. Recently, we showed dynamics allows formation open conformers can be sampled by its structurally diverse redox partners a CYP-isoform dependent manner. To...
Abstract Cytochromes P450 constitute a superfamily of haem‐thiolate mono‐oxygenases that are involved in the oxidative metabolism lipophilic subtrates. These enzymes require association with cytochrome reductase (CPR) to achieve optimal activities. We have expressed human CYP1A1 under POX2 promoter (pPOX2–CYP1A1) Y. lipolytica , or without overproduction CPR ICL (pICL–CPR) (pPOX2–CPR). Activity was analysed by conversion hydroxyresorufin resorufin. Strain JMY330 and JMY330–CPR present no...
4-hydroxybenzoic acid (pHBA) is an important industrial precursor of muconic and liquid crystal polymers whose production based on the petrochemical industry. In order to decrease our dependency fossil fuels improve sustainability, microbial engineering a particularly appealing approach for replacing traditional chemical techniques. The optimization strains, however, still highly constrained by screening stage. Biosensors have helped alleviate this problem decreasing time as well enabling...