A5050319678- MicroRNA in disease regulation
- Chromium effects and bioremediation
- Heavy Metal Exposure and Toxicity
- Cancer-related molecular mechanisms research
- BRCA gene mutations in cancer
- Circular RNAs in diseases
- Drug Transport and Resistance Mechanisms
- Pharmacogenetics and Drug Metabolism
- DNA Repair Mechanisms
- Carcinogens and Genotoxicity Assessment
- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- Estrogen and related hormone effects
- Potato Plant Research
- Thyroid Cancer Diagnosis and Treatment
- Heavy metals in environment
- Amyotrophic Lateral Sclerosis Research
- Salivary Gland Tumors Diagnosis and Treatment
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Computational Drug Discovery Methods
- biodegradable polymer synthesis and properties
- RNA modifications and cancer
- Youth, Drugs, and Violence
- Effects and risks of endocrine disrupting chemicals
National Institute of Health Dr. Ricardo Jorge
2019-2024
Universidade Nova de Lisboa
2011-2024
Universidade Lusófona
2024
Inova Design Solutions (United Kingdom)
2024
Universidade Federal de Roraima
2024
University of Lisbon
2009-2023
Mayo Clinic
2022
WinnMed
2022
Goethe University Frankfurt
2022
NILU
2022
The EU human biomonitoring initiative, HBM4EU, aims to co-ordinate and advance (HBM) across Europe. Within its remit, the project is gathering new, policy relevant, EU-wide data on occupational exposure relevant priority chemicals developing new approaches for biomonitoring. In this manuscript, hexavalent chromium [Cr(VI)] study design presented as first example of HBM4EU approach. This involves eight European countries plans recruit 400 workers performing Cr(VI) surface treatment e.g....
Exposure to hexavalent chromium [Cr(VI)] may occur in several occupational activities, e.g., welding, Cr(VI) electroplating and other surface treatment processes. The aim of this study was provide EU relevant data on exposure support the regulatory risk assessment decision-making. In addition, capability validity different biomarkers for were evaluated. involved nine European countries 399 workers industry sectors with exposures such as bath plating, applying or removing paint tasks. We also...
A study was conducted within the European Human Biomonitoring Initiative (HBM4EU) to characterize occupational exposure Cr(VI). Herein we present results of biomarkers genotoxicity and oxidative stress, including micronucleus analysis in lymphocytes reticulocytes, comet assay whole blood, malondialdehyde 8-oxo-2′-deoxyguanosine urine. Workers from several Cr(VI)-related industrial activities controls (within company) non-industrial (outwith environments were included. The significantly...
The altered activity of drug metabolism enzymes (DMEs) is a hallmark chemotherapy resistance. Cytochrome P450s (CYPs), mainly CYP3A4, and several oxidoreductases are responsible for Phase I doxorubicin (DOX), an anthracycline widely used in breast cancer (BC) treatment. This study aimed to investigate the role DMEs involved first stages acquisition DOX-resistance BC cells. For this purpose, expression 92 DME genes specific CYP-complex activities were assessed either sensitive (MCF-7 parental...
Abstract Amyotrophic lateral sclerosis (ALS) is a progressive motor neuron disease that affects nerve cells in the brain and spinal cord, causing loss of muscle control, atrophy later stages, death. Diagnosis has an average delay 1 year after symptoms onset, which impairs early management. The identification specific biomarker could help decrease diagnostic delay. MicroRNA (miRNA) expression levels have been proposed as ALS biomarkers, altered function reported pathogenesis. aim this study...
In recent years numerous studies have indicated the importance of microRNAs (miRNA/miRs) in human pathology. Down syndrome (DS) is most prevalent survivable chromosomal disorder and attributed to trisomy 21 subsequent alteration dosage genes located on this chromosome. A number miRNAs are overexpressed down syndrome, including miR‑155, miR‑802, miR‑ 125b‑2, let‑7c miR‑99a. This overexpression may contribute neuropathology, congenital heart defects, leukemia low rate solid tumor development...
NADPH cytochrome P450 oxidoreductase (CPR) is the obligatory electron supplier in sustaining activity of microsomal (CYP). The variant nature isoform specific proximal interface CYPs implies that CPR capable multiple degenerated interactions with for transfer, through different binding mechanisms, which are still not well understood. Recently, we showed dynamics allows formation open conformers can be sampled by its structurally diverse redox partners a CYP-isoform dependent manner. To...
We wished to examine the effectiveness of tibial lengthening using a two ring Ilizarov frame in skeletally immature patients. This is potentially biomechanically unstable construct which risks loss axial control.We retrospectively reviewed consecutive series 24 boys and 26 girls, with mean age 8.6 years (4 14), who underwent 52 procedures follow-up 4.3 (4.0 16.9). Tibial alignment was measured before after treatment joint orientation lines from knee calculation oblique plane axis.The...
NADPH-cytochrome P450 reductase (CPR) is the unique redox partner of microsomal cytochrome P450s (CYPs). CPR exists in a conformational equilibrium between open and closed conformations throughout its electron transfer (ET) function. Previously, we have shown that electrostatic flexibility properties hinge segment are critical for ET. Three mutants human were studied (S243P, I245P R246A) combined with representative drug-metabolizing CYPs (isoforms 1A2, 2A6 3A4). To probe effect these...
Cancer drug resistance (CDR) is a major problem in therapeutic failure. Over 90% of patients with metastatic cancer present CDR. Several mechanisms underlie CDR, including the increased expression efflux ABC transporters and epigenetic phenomena. Nevertheless, topic that not usually addressed mechanism underlying loss CDR once challenge to these cells withdrawn. A KCR cell line (doxorubicin-resistant, expressing ABCB1) was used induce by withdrawing doxorubicin culture medium. ABCB1 activity...
Genetic testing could play a critical role in diagnosis and prognosis of acute pancreatitis (AP) guide effective therapeutic interventions. We hypothesized that genetic polymorphisms apoptosis oxidative stress genes determine incidence or severity AP.We conducted hospital-based case-control study white Portuguese population (133 AP patients 232 age- sex-matched healthy controls) to evaluate the 15 gene (2 deletions 13 single nucleotide [SNPs]) (GSTM1, GSTT1, GSTP1) (CASP7, CASP8, CASP9,...
Experimental and epidemiologic studies have shown that lead (Pb) is able to induce epigenetic modifications, such as changes in DNA methylation profiles, chromatin remodeling, well the expression of non-coding RNAs (ncRNAs). However, very little known about interactions between microRNAs (miRNAs) status individuals exposed metal. The aim present study was investigate impact hsa-miR-148a on status, 85 workers Pb. Blood plasma levels (BLL PLL, respectively) were determined by ICP-MS;...
The incidence of thyroid cancer (TC), particularly well-differentiated forms (DTC), has been rising and remains the highest among endocrine malignancies. Although ionizing radiation (IR) is well established on DTC aetiology, other environmental genetic factors may also be involved. DNA repair single nucleotide polymorphisms (SNPs) could former, helping in explaining high incidence. To further clarify role SNPs susceptibility, we analyzed 36 27 genes a population 106 DTCs corresponding...
Breast cancer (BC) is the most common in women. In contrast, male BC about 100 times less than women, being considered a rare disease. Male may be distinctive subtype of and available data seems to indicate that has higher dependence on genetic variants female BC. Nevertheless, same prognostic predictive markers are used determine optimal management strategies for both Several studies have assessed role polymorphisms (SNPs) DNA repair genes susceptibility. However, scarce. Thus, current...
Aim: ABCB1 is a major player in cancer drug resistance. The purpose of this study was to functionally assess the regulation activity doxorubicin-resistant breast cell line by miR-200c and miR-203. Methods: Human carcinoma lines MCF-7 (Doxorubicin-sensitive not expressing ABCB1) KCR (Doxorubicin-resistant were used evaluate expression levels miR-203 Real-time quantitative PCR (RT-qPCR). effects transient ectopic miRNA-200c on cells verified RT-qPCR Western Blot. extrusion pump analyzed...
Radioiodine therapy with 131I remains the mainstay of standard treatment for well-differentiated thyroid cancer (DTC). Prognosis is good but concern exists that 131I-emitted ionizing radiation may induce double-strand breaks in extra-thyroidal tissues, increasing risk secondary malignancies. We, therefore, sought to evaluate induction and 2-year persistence micronuclei (MN) lymphocytes from 26 131I-treated DTC patients potential impact nine homologous recombination (HR), non-homologous...