Adipose-derived mesenchymal stem cells (ASCs) release factors beneficial for islets in vitro and protect against hyperglycemia rodent models of diabetes. Oxygen tension has been shown to induce metabolic changes alter ASCs' soluble factors. The effects hypoxia on the antidiabetic properties ASCs have not explored. To investigate this, we incubated human 48 h 21% (normoxia) or 1% O2 (hypoxia) compared viability, cell growth, surface markers, differentiation capability, conditioned media (CM)....

Coronavirus disease 2019 (COVID-19) displays clinical heterogeneity, but little information is available for patients with mild or very early disease. We aimed to characterize biomarkers that are useful in discriminating the hospitalization risk a COVID-19 cohort from Northern Italy during first pandemic wave. enrolled and followed four weeks 76 symptomatic SARS-CoV-2 positive age/sex-matched healthy controls. Patients were discharged (n.42), remaining hospitalized (n.34). Blood was...

BACKGROUND: This study aimed to compare platelet (PLT) quality during storage of buffy coat (BC) PLT concentrates (PCs), prepared either manually or by the automated OrbiSac system (Gambro BCT). STUDY DESIGN AND METHODS: Following overnight at 20 22°C, five BCs were pooled with 300 mL additive solution. Twenty‐one PCs produced (M‐PCs) and 21 (A‐PCs). Swirling, count, mean volume, blood gas analyses, potassium, glucose, lactate assessed. Expression activation markers CD42a, CD62P, PAC‐1 was...

ChAdOx1 nCoV-19 vaccination has been associated with the rare side effect; vaccine-induced immune thrombotic thrombocytopenia (VITT). The mechanism of thrombosis in VITT is high levels neutrophil extracellular traps (NETs). present study examines whether key markers for NETosis, such as H3-NETs and calprotectin, well syndecan-1 endotheliopathy, can be used prognostic factors to predict severity complications vaccination. Five patients VITT, 10 prolonged symptoms cutaneous hemorrhages but...

AbstractA large part of native meningococcal lipopolysaccharide (LPS), i.e., LPS integrated in the outer cell membrane, is released form 'blebs' from surplus membrane material. In present study we investigated effects purified vesicles (OMVs) on blood platelet-platelet and platelet-leukocyte interactions. Citrated whole was stimulated vitro with equal amounts (on a weight basis) OMV-integrated LPS, (P-LPS) same strain E. coli -LPS. The samples were analyzed by flow cytometry. Upon OMV...

Background The novel A/J Min/+ mouse, which is a model for human Familial Adenomatous Polyposis (FAP), develops spontaneously multiple adenocarcinomas in the colon as well small intestine. Agaricus blazei Murill (AbM) an edible Basidiomycetes mushroom that has been used traditional medicine against cancer and other diseases. contains immunomodulating β-glucans shown to have antitumor effects murine models. Andosan™ water extract based on AbM (82%), but it also medicinal mushrooms Hericeum...

Since Agaricus blazei Murill (AbM) extract reduced specific IgE and ameliorated a skewed Th1/Th2 balance in mouse allergy model, it was tested blood donors with self-reported, IgE-positive, birch pollen and/or asthma. Sixty recruited were randomized placebo-controlled, double-blinded study pre-seasonal, 7-week, oral supplementation the AbM-based AndosanTM. Before after season, questionnaires answered for allergic rhino-conjunctivitis, asthma, medication; serum measured, Bet v 1-induced...

Two novel enzyme-linked immunosorbent assays (ELISAs), designed to detect complexes containing DNA, leucocyte calprotectin and S100A12 proteins, were generated for improved specificity rapid measurement of neutrophil extracellular traps (NETs). The applied on plasma serum samples from blood donors establishment reference values, patients with multiple myeloma (MM) or rheumatoid arthritis (RA) in order examine putatively increased values the two different inflammatory conditions. Although...

Coronavirus disease 2019 (COVID-19) displays clinical heterogeneity, but little information is available for patients with mild or very early disease. We aimed to characterize biomarkers that are useful discriminating the hospitalization risk in a COVID-19 cohort from Northern Italy during first pandemic wave. enrolled and followed four weeks 76 symptomatic SARS-CoV-2 positive age/sex-matched healthy controls. Patients were discharged (n.42), remaining hospitalized (n.34). Blood was...

To improve platelet (PLT) quality, hyperconcentrated PLT concentrates (hcPCs) were compared to standard (stdPCs) in two different additive solutions, T-Sol and PAS-27a. PAS-27a differs from by containing glucose, phosphate, potassium, magnesium, bicarbonate.PLTs harvested apheresis twice 14 donors; each unit was divided into two. Four units donor produced: hcPCs, 2000 x 10(9) per L or PAS-27a; stdPCs, 1400 65 percent 35 acid citrate dextrose-plasma. On Days 1 through 4, swirling scored...

BACKGROUND: The objective of this study was to evaluate the suitability cord blood (CB) as a source red cells (RBCs) for autologous transfusion. STUDY DESIGN AND METHODS: CB collected in 150‐mL storage containers with citrate phosphate dextrose (CPD) anticoagulant and stored either saline, adenine, glucose, mannitol (SAG‐M; n = 18) or phosphate, guanosine, (PAGGS‐M; 35 days at 4°C. Hematologic status hemolysis were studied. lipopolysaccharide (LPS)‐induced production tumor necrosis factor...

Persisting inflammation has been discovered in lungs and other parenchymatous organs of some COVID-19 convalescents. Calprotectin, neutrophil extracellular traps (NETs), syndecan-1 neopterin are general key inflammatory markers, systemically enhanced levels them may remain after the infection. These markers were therefore measured serum samples 129 convalescent 27 healthy blood donors or employees at Oslo Blood bank, Norway. Also antibodies against SARS-CoV-2 nucleocapsid antigen measured,...

BACKGROUND: Lipids and other biologically active substances accumulate in platelet concentrates (PCs) during storage. Some of these have been suggested to modulate immune responses play a pathogenic role the development transfusion‐related acute lung injury. This study compared content impact some biological response modifiers PCs treated with pathogen reduction (PR) technology nontreated PCs. STUDY DESIGN AND METHODS: Apheresis (n = 12) were split two: one was subjected PR treatment...

Abstract Background Refractory patients need to be provided with HLA‐matched platelets (PLTs), which require time‐consuming cross‐matching. Treatment of PLTs citric acid leads denaturation the HLA Class I complexes without significant damage PLTs. depleted could alternatively used for transfusion. These have verified normal function up 4–6 h after treatment. Materials and Methods Buffy coat (BC) PLT concentrates were by incubation in acid. The days acid‐treatment, surface expression...

Abstract Background Patients can form antibodies to foreign human leukocyte antigen (HLA) Class I antigens after exposure allogeneic cells. These anti‐HLA class bind transfused platelets (PLTs) and mediate their destruction, thus leading PLT refractoriness. with refractoriness need HLA‐matched PLTs, which require expensive HLA typing of donors, antibody analyses patient sera and/or crossmatching. An alternative approach is reduce expression using a brief incubation in citric acid on ice at...

Platelets contain three types of secretory granules, dense α-granules and lysosomes, which are characterized by their different contents. Dense granule α-granule secretion appear to be similar in responsiveness dose agonists, whereas lysosomal is observed only with higher doses strong agonists such as thrombin. Recently, the advent flow cytometry, surface expression membrane proteins, claimed specific for type, has come into use a monitor secretion. Expression CD62 (PADGEM) particular become...

Agaricus blazei Murill is an edible mushroom of the Basidiomycetes family, which has been found to contain a number compounds with antitumor properties, such as proteoglycans and ergosterol. In present investigation, we show that commercial product Andosan, contains 82.4% Murill, together medicinal mushrooms Hericium erinaceus (14.7%) Grifola frondosa (2.9%), cytotoxic effect on primary myeloma cells, other cell lines, leukemia lines in vitro. Although exact content hence mechanisms action...

Abstract Introduction Human hematopoietic stem cells (HSCs) have been clinically used for transplantation and gene cellular therapy more than 4 decades. However, this use is limited because of the challenges in ex vivo culturing HSCs. The major hurdle to amplify these without losing their self-renewing property. Methods In our study, we tested 3′,4′-dimethoxyflavone (3′4′-DMF) valproic acid (VPA) on expansion HSCs under both normoxic (20% O 2 ) hypoxic (1% conditions. 3′4′-DMF a widely...

Flow cytometry was used to study the expression of leukocyte adhesion molecules CD11a, CD11b, CD11c, CD14, and CD62L (L‐selectin) production reactive oxygen species (ROS) in an ex vivo human whole‐blood system stimulated with lipopolysaccharide‐containing outer membrane vesicles (LPS‐OMV) from N. meningitidis. Results demonstrated a dose‐dependent increase surface CD11c CD14 granulocytes monocytes (maximal at 30–120 min) upon OMV‐LPS challenge, whereas heavily downregulated min). The...

Alloimmunization is a common phenomenon after transfusion, with an estimated incidence of 0.5% increasing to 20-60% in chronically transfused patients. In recently patients, serological typing can be hampered by mixed field agglutination. We established RT-PCR methods for RHD, RHC/c and RHE/e using mRNA from reticulocytes. Molecular was performed soon 51 separate mismatched transfusion events involving 30 Accurate identification the patients' phenotype confirmed all cases. Reticulocyte...