A5089784232- Parasitic Diseases Research and Treatment
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- Parasite Biology and Host Interactions
- Rabies epidemiology and control
- Mosquito-borne diseases and control
- Insects and Parasite Interactions
- Trypanosoma species research and implications
- Dermatological diseases and infestations
- Parasites and Host Interactions
- Vector-Borne Animal Diseases
- Bartonella species infections research
- Parasitic Infections and Diagnostics
- Blood properties and coagulation
- Research on Leishmaniasis Studies
- Insect and Pesticide Research
- Helminth infection and control
- Antifungal resistance and susceptibility
- Leptospirosis research and findings
- Mollusks and Parasites Studies
- Complement system in diseases
- Insect Utilization and Effects
- Virology and Viral Diseases
- Genetics, Aging, and Longevity in Model Organisms
IDEXX Laboratories (United States)
2014-2023
Institute of Medical Sciences
2018
Baxter (United States)
2011-2017
North Carolina State University
2011-2017
Colorado State University
2015
University of Rhode Island
2015
IDEXX Laboratories (France)
2004-2014
Town and Country Veterinary Clinic
2014
Oklahoma State University
2014
Heska (United States)
1997-2002
Rhipicephalus sanguineus is a ubiquitous tick responsible for transmitting Ehrlichia canis and most likely Anaplasma platys to dogs, as either single or co-infections. The objective of this study was assess the effects simultaneous sequential experimental infections with E. A. on hematological serological parameters, duration infection, efficacy doxycycline therapy in dogs infected one both organisms. Six per group were uninfected, infected, co-infected, challenged at day 112 post-infection...
A population of 731 naturally exposed pet dogs examined at a private practice in Baxter, Minnesota, an area endemic for Lyme disease and anaplasmosis, was tested by serological molecular methods evidence exposure to or infection with selected vector-borne pathogens. Serum samples were enzyme-linked immunosorbent assay (ELISA) Anaplasma phagocytophilum, Borrelia burgdorferi, Ehrlichia canis antibodies Dirofilaria immitis antigen. Blood from 273 also analyzed polymerase chain reaction (PCR)...
To evaluate the performance of an in-clinic ELISA designed for detection heartworm antigen and antibodies against 5 tick-borne pathogens.Validation study.1,601 serum or matched serum, plasma, blood samples from dogs.Samples were tested Dirofilaria immitis (heartworm) Anaplasma phagocytophilum, platys, Borrelia burgdorferi, Ehrlichia canis, ewingii by means ELISA. Evaluation assay sensitivity specificity, agreement results among sample types, cross-reactivity E canis antigens in with...
To evaluate the sensitivity and specificity of a commercially available in-clinic ELISA for detection heartworm infection tick-borne diseases in dogs.846 serum, plasma, or blood samples obtained from dogs.Samples were evaluated via to detect antibodies against Anaplasma phagocytophilum, Ehrlichia canis, Borrelia burgdorferi Dirofilaria immitis (heartworm) antigen. True immunologic status was assessed by use results necropsy, an antigen assay D immitis, immunofluorescence western blot...
The geographic distribution of canine infection with vector-borne disease agents in the United States appears to be expanding. To provide an updated assessment trends Dirofilaria immitis, Borrelia burgdorferi, Ehrlichia spp., and Anaplasma we evaluated results from average 3,588,477 dogs tested annually by veterinarians throughout 2010 – 2012. As earlier summary report, percent positive test varied agent region, antigen D. immitis antibody spp. most commonly identified Southeast (2.9% 3.2%,...
Canine vector-borne diseases (CVBDs) represent a wide group of major significance for canine health. In addition to their veterinary importance, many these are great zoonotic concern, posing risk potential transmission humans. To date, there has been scant knowledge regarding the prevalence, distribution and factors CVBDs in Greece. Therefore, objectives present study were update current on seroprevalence Dirofilaria immitis, Anaplasma spp., Ehrlichia spp. Borrelia burgdorferi (sensu lato)...
Background Long‐term microscopic agglutination test ( MAT ) results after vaccination with 4‐serovar L eptospira vaccines are not available for all used in client‐owned dogs. Hypothesis/Objectives To determine antibody responses of dogs given 1 4 commercially vaccines. Animals Healthy (n = 32) no history at least the previous year. Methods Dogs were on week 0 and then approximately 3 52. Sera collected before vaccine administration within days 3, 2 4, weeks 7, 15, 29, 52, 56. Antibody titers...
Tick-borne pathogens cause a spectrum of disease manifestations in both dogs and humans. Recognizing regional temporal shifts exposure are important as tick distributions change. To better delineate to canine tick-borne pathogens, an expanded set species-specific peptides were used detect Anaplasma phagocytophilum (Aph), platys (Apl), Ehrlichia canis (Ec), chaffeensis (Ech), ewingii (Eew), Borrelia burgdorferi (Bb) antibodies serum.Archived serum samples (n=6,582) collected during 2008-2010...
Vaccination with irradiated infective larvae induces partial protective immunity to infection the filarial nematode Brugia malayi in jirds. Prior studies have shown that such immunization stimulates a much stronger antibody response recombinant and native paramyosin than seen after normal infection. To determine whether vaccination could induce larval challenge, jirds were immunized either B. maltose binding protein (BM5-MBP) (fusion of maltose-binding protein), MBP alone, or Dirofilaria...
We evaluated the serological and molecular prevalence of selected organisms in 145 dogs during late spring (May/June) 2005 88 winter (February) 2007 from Hopi Indian reservation. Additionally, 2005, 442 ticks attached to were collected identified as Rhipicephalus sanguineus. Infection with or exposure at least one organism was detected 69% 66% May/June February 2007, respectively. Exposure spotted fever group (SFG) rickettsiae 66.4% (2005) 53.4% (2007) dogs, but rickettsial DNA not using...
Eighteen clinically ill dogs, naturally infected with Anaplasma phagocytophilum, were examined at a veterinary practice in Baxter, Minnesota. A clinical examination, complete blood cell count, enzyme- linked immunosorbent assay (ELISA) for Borrelia burgdorferi, and Ehrlichia canis antibodies Dirofilaria immitis antigen, polymerase chain reaction test phagocytophilum DNA obtained all dogs. Physical examination findings included fever, arthropathy, lymphadenopathy, epistaxis, acute gastritis,...
With the exception of Bartonella spp. or Cytauxzoon felis, feline vector-borne pathogens (FVBP) have been less frequently studied in North America and are generally under-appreciated as a clinical entity cats, compared to dogs people. This study investigated selected FVBP seroreactivity PCR prevalence cats using archived samples. Feline blood samples submitted Vector Borne Diseases Diagnostic Laboratory (VBDDL) at Carolina State University College Veterinary Medicine (NCSU-CVM) between 2008...
Considering the zoonotic potential of tick-borne disease (TBD) agents and fact that dogs may act as sentinels for human infection, aim present study was to determine seroprevalence TBD risk factors exposure in two different canine populations from Parana State, Southern Brazil. A total 138 dog serum samples urban (UA) (n=68) rural (RA) (n=70) areas were tested with commercial ELISA rapid test forAnaplasma phagocytophilum, Ehrlichia canisand Borrelia burgdorferi antibodies indirect...
SUMMARY The aims of this study were to determine the seroprevalence Ehrlichia spp. and risk factors for exposure in a restricted population dogs, horses, humans highly exposed tick bites Brazilian rural settlement using commercial ELISA rapid test two indirect immunofluorescent assays (IFA) with E. canis chaffeensis crude antigens. Serum samples from 132 16 horses 100 used. Fifty-six out (42.4%) dogs seropositive canis. Dogs > one year more likely be than ≤ (p = 0.0051). Ten/16 (62.5%)...
Visceral leishmaniasis (VL) is a vector borne zoonotic disease endemic in humans and dogs Brazil. Due to the increased risk of human infection secondary presence infected dogs, public health measures Brazil mandate testing culling dogs. Despite this important relationship between canine infection, little known about what makes dog reservoir progress clinical illness, significantly tied infectiousness sand flies. Dogs areas are exposed many tick-borne pathogens, which likely alter immune...
Vector-borne pathogens are emerging concerns in multiple regions of Canada. Determining regional prevalence canine vector-borne and documenting change will improve clinician awareness, enable targeted prevention, enhance diagnosis ideally reduce the risk disease. Study objectives were to: (i) estimate positive test results from samples submitted Canada; (ii) assess over time, baseline (2008) to 2015; (iii) pathogen co-infections.This repeat cross-sectional study evaluated 753,468 for D....
The term "endemic normal" in the context of filariasis refers to people who are amicrofilaremic and free clinical signs or symptoms despite regular exposure parasite. Some sera from endemic normals contain soluble Wuchereria bancrofti antigens that detectable by enzyme-linked immunosorbent assay. We now report evidence filarial antigenemia these is not an artifact it indicative active W. infection. Filarial was first detected within one month onset microfilarial patency experimentally...
Abstract A consistent and reproducible method is described for isolating pure populations of microfilariae Litomosoides carinii, Brugia pahangi, B. malayi Dipetalonema viteae , free cells, from blood, by density gradient centrifugation on Percoll in 0.25M sucrose. The recovery the was 85 to 97%.