Two versatile yet simple methods, colorimetric and spectrofluorimetric, were utilized for the quantitation of nonchromophore neomycin using silver nanoparticles modified with fluorescein. Fluorescein was excited at 485 nm (emission 515 nm); when it is deposited on surface nanoparticles, its fluorescence intensity quenched. Neomycin restores level by displacing fluorescein from nanoparticle binding sites. When reacts fluorescein, causes reduction nanoparticles' plasmon resonance band 395...

ABSTRACT The implementation of core–shell particles in HPLC has many advantages analytical methodologies, including enhancement sensitivity and resolution. Moreover, high flow rates short analysis times could be used to achieve efficient separations. A sensitive specific UHPLC–MS/MS method was developed validated for the simultaneous quantification apixaban (APX) rivaroxaban (RVX) rat plasma. This designed investigate potential pharmacokinetic (PK) interactions between dexamethasone (DEX)...

ABSTRACT One of the greatest threats to human health in modern times and a significant global concern is antibiotic resistance. The combination carbapenemase inhibitor ceftazidime with tebipenem may be high benefit for fighting resistant bacteria. By using rapid extremely sensitive HPLC‐UV technique, simultaneous estimation rats was possible. Acetonitrile used protein precipitation procedure prepare samples. best chromatographic conditions were established by applying C 18 INERTSIL ODS‐3...

The endocrine system of vitamin D regulates about 3 % the human genome. Vitamin exerts its actions via a nuclear receptor (VDR) which in turn insulin secretion from pancreas. VDR gene polymorphisms could have an impact on how autoimmune illnesses like Type 1 diabetes mellitus (T1DM) develop. We aimed to explore relation between T1DM and Egyptian diabetic children their siblings. Enzyme-linked immunosorbent assay was used quantify 25(OH) study, had 179 participants (group = 85 children, group...

Abstract In this study, the simultaneous determination of aspirin, clopidogrel, and either atorvastatin or rosuvastatin in their fixed‐dose combination (FDC) formulations has been reported. As a straightforward substitute for employing distinct models each component, UV spectrophotometry was applied with chemometric approaches artificial neural networks to achieve this. Three techniques, including principal component regression (PCR), partial least‐squares (PLS), classical (CLS), were...

Abstract Three-levels Box-Behnken design was used in the experimental approach for optimization of chromatographic parameters to achieve optimum resolution and sharp peak shape within a reasonable run time. A method that is sensitive, reliable, selective constructed validated simultaneous measurement combination therapy contains blood-thinning cholesterol-lowering compounds. The four cited drugs namely, aspirin (ASP), clopidogrel (CLP), atorvastatin (ATV) rosuvastatin (ROS) were estimated...

Helicobacter pylori infection is a common cause of peptic ulcer disease and dyspepsia. In addition, it may result in gastric cancer mucosa associated lymphoid tissue lymphoma. First-line therapy usually consists triple containing clarithromycin or amoxicillin, one the proton pump inhibitors, metronidazole tinidazole. addition to therapy, an analgesic required relieve pain such as naproxen.A sensitive selective method needs be developed validated for simultaneous determination four drugs...

Hearing impairment is frequent in patients with end-stage renal disease (ESRD). We aimed to assess the prevalence of hearing children on regular hemodialysis and transplantation.Transient-evoked otoacoustic emissions (TEOAEs) has been done for 80 40 transplant.In group, TEOAEs showed that 53.8% had affection, it was significantly related dialysis duration, adequacy, vascular access infection, hepatitis C virus (HCV) ototoxic drugs (p = 0.001, 0.037, 0.011, 0.004, 0.030, 0.007, 0.044,...

Abstract The green profile of the developed method is assessed and compared with previously reported methods. Percutaneous coronary intervention a procedure where catheter utilized to place stent in order facilitate opening blood vessels heart. Triple antithrombotic therapy includes oral anticoagulation as warfarin dual antiplatelet (composed aspirin clopidogrel bisulfate). aim current study was evaluate pharmacokinetic parameters ASP, WAR CLP investigate possible interaction between three...

More than 2.9 million people have died as a result of the global demographic impact coronavirus illness 2019 (COVID-19). Numerous antiviral and anti-inflammatory medications FDA approval to treat COVID-19 patients. For simultaneous determination utilized (Remdesivir, Moxifloxacin, Dexamethasone, Apixaban, paracetamol) in their dosage forms, sensitive technique has been developed validated. The aforementioned were separated quantified with help experimental design. Box-Behnken design was used...

Abstract Status epilepticus (SE) is considered the second most frequent neurological emergency. Its therapeutic management performed using sequential antiepileptic drug regimens. Diazepam (DIA), midazolam (MID), phenytoin (PHT) and phenobarbital (PB) are four drugs of different classes used sequentially in SE. A sensitive, selective, accurate precise method was developed validated for simultaneous determination human plasma. Their separation quantification were achieved ultra‐performance...

Abstract Background Rufinamide is a triazole derivative that structurally dissimilar to other marketed antiepileptic drugs, has been assumed marketing authorization, by the European Union and FDA, for use as complementary therapy seizures associated with Lennox-Gastaut syndrome. Objective This work concerned development of two methods determination rufinamide (RUF) in presence 1-[(2,6-difluorophenyl)methyl]-1H-1,2,3-triazole-4 carboxylic acid its alkaline degradation product dosage form....

Objective: Lennox–Gastaut syndrome (LGS) is mainly treated with antiepileptic drugs (AEDs) but using one AED not sufficient to relieve all or even most patients. A combination of agents usually preferred. In the current study, an isocratic, selective, sensitive, precise, and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for simultaneous determination rufinamide (RUF), lamotrigine (LAM), clonazepam (CLO), valproic acid (VAL), diazepam (DIA)...

Abstract Rufinamide (RF), antiepileptic drug, is biotransformed to inactive metabolite. Frequent plasma monitoring required for dose adjustment. This work concerned with the development and validation of a sensitive selective RP-HPLC method quantitative determination RF in spiked human presence its main Lacosamide was selected as internal standard. Preparation samples involved precipitation proteins using methanol. Isocratic elution mode applied chromatographic separation performed on...

Objectives: The purpose of this research was to determine how COVID-19 infection affects the sexual health and menstrual cycle reproductive-aged women. Patients Methods: Beginning in May 2022, 384 female patients from Obstetrics Gynecology department's outpatient clinic at Beni Suef University were included cross-sectional study. study lasted for six months. Results: In present study, a total 148 sexually active women, or 40.7% sample, exhibited dysfunction, as determined by FSFI score cut...

Abstract Artificial neural networks (ANNs) are biologically inspired algorithms designed to simulate the way in which human brain processes information. In sample preparation for bioanalysis, liquid–liquid extraction (LLE) represents an important step with solvent selection is key laborious step. current work, a robust and reliable ANNs model LLE prediction was generated could predict suitable analyte extraction. The developed takes set of chosen descriptors cited as input predicts...

Aim: An eco-friendly ultra-performance liquid chromatography-tandem mass spectrometry method was developed to study the pharmacokinetics of rivaroxaban and ticagrelor in rat plasma, utilizing moxifloxacin as an internal standard. The food-drug interaction between grapefruit juice these drugs also investigated. Methods: Liquid-liquid extraction used. A nonporous stationary phase Agilent® Poroshell 120EC C18 column used with methanol: 0.1% aqueous formic acid (95:5 v/v) a mobile phase....

Abstract Background Neonatal sepsis is the major cause of morbidity and mortality in neonates. Frequency bacterial affected by seasonal variations. This study was done to rule out effects variation on neonatal determine isolated agents their antibiotic sensitivity profile. Results cross-sectional included100 neonates with suspected sepsis, conducted during summer winter seasons. High frequencies morbidities were observed like poor feeding (98%), Jaundice (82%), pallor (76%), respiratory...

A brand-new class of anti-infective drugs that work against bacteria, viruses, and protozoan parasites is nitazoxanide related thiazolides. Thiazolides have also been shown to cause cell cycle arrest apoptotic death in cancer cells recent years. In this study, an eco-friendly, spectrofluorimetric technique verified, easy, sensitive has proposed for quantifying (NTZ), a broad-spectrum antiparasitic drug. When NTZ reduced with zinc (Zn) powder acidic media, highly fluorescent product produced....