A5048664236- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Wound Healing and Treatments
- Diabetic Foot Ulcer Assessment and Management
- Extracellular vesicles in disease
- Blood Coagulation and Thrombosis Mechanisms
- Mesenchymal stem cell research
- Signaling Pathways in Disease
- Congenital heart defects research
- Pressure Ulcer Prevention and Management
- RNA modifications and cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Ethics in Clinical Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cardiovascular Disease and Adiposity
- RNA Interference and Gene Delivery
- Congenital Heart Disease Studies
- Organ Transplantation Techniques and Outcomes
- Tissue Engineering and Regenerative Medicine
- Cardiac Structural Anomalies and Repair
- Scientific Computing and Data Management
- Bioinformatics and Genomic Networks
- Pulmonary Hypertension Research and Treatments
- Nuclear Structure and Function
Stanford University
2019-2023
Cardiovascular Institute of the South
2019-2023
Harvard University
2019-2021
Brigham and Women's Hospital
2021
Medizinische Hochschule Hannover
2011-2020
Beth Israel Deaconess Medical Center
2018-2020
Stanford Medicine
2019
Imperial College London
2013-2015
Heart and Diabetes Center North Rhine-Westphalia
2015
Ruhr University Bochum
2015
In response to stress, the heart undergoes extensive cardiac remodeling that results in fibrosis and pathological growth of cardiomyocytes (hypertrophy), which contribute failure. Alterations microRNA (miRNA) levels are associated with dysfunctional gene expression profiles many cardiovascular disease conditions; however, miRNAs have emerged recently as paracrine signaling mediators. Thus, we investigated a potential miRNA crosstalk between fibroblasts found secrete miRNA-enriched exosomes....
Pathological growth of cardiomyocytes (hypertrophy) is a major determinant for the development heart failure, one leading medical causes mortality worldwide. Here we show that microRNA (miRNA)-212/132 family regulates cardiac hypertrophy and autophagy in cardiomyocytes. Hypertrophic stimuli upregulate cardiomyocyte expression miR-212 miR-132, which are both necessary sufficient to drive hypertrophic MiR-212/132 null mice protected from pressure-overload-induced whereas cardiomyocyte-specific...
Objective— MicroRNAs are a class of small ribonucleotides regulating gene/protein targets by transcript degradation or translational inhibition. Transforming growth factor-β (TGF-β) is involved in cardiac fibrosis partly stimulation endothelial-to-mesenchymal transition (EndMT). Here, we investigated whether microRNA (miR)-21, enriched fibroblasts and general fibrosis, has role EndMT. Methods Results— TGF-β treatment endothelial cells significantly increased miR-21 expression induced EndMT...
Abstract Despite proven efficacy of pharmacotherapies targeting primarily global neurohormonal dysregulation, heart failure (HF) is a growing pandemic with increasing burden. Treatments mechanistically focusing at the cardiomyocyte level are lacking. MicroRNAs (miRNA) transcriptional regulators and essential drivers disease progression. We previously demonstrated that miR-132 both necessary sufficient to drive pathological cardiomyocytes growth, hallmark adverse cardiac remodelling....
Circulating microRNAs (miRNAs/miRs) are used as novel biomarkers for diseases. miR-21, miR-126, and miR-210 known to be deregulated in vivo or vitro under diabetic conditions.The aim of this study was investigate the circulating plasma urine from pediatric patients with type 1 diabetes link our findings cardiovascular nephropathy risk factors children diabetes.miR-21, concentrations were measured quantitative RT-PCR samples 68 79 sex- age-matched controls.The consisted clinical...
Objective— MicroRNAs (miRNA/miR) are stably present in body fluids and increasingly explored as disease biomarkers. Here, we investigated influence of impaired wound healing on the plasma miRNA signature their functional importance patients with type 2 diabetes mellitus. Approach Results— array profiling identified 41 miRNAs significantly deregulated diabetic controls when compared mellitus–associated peripheral arterial chronic wounds. Quantitative real-time polymerase chain reaction...
Nonhealing diabetic foot ulcers (DFUs) are characterized by low-grade chronic inflammation, both locally and systemically. We prospectively followed a group of patients who either healed or developed nonhealing DFUs. Serum forearm skin analysis, at the protein expression transcriptomic level, indicated that increased factors such as interferon-γ (IFN-γ), vascular endothelial growth factor, soluble cell adhesion molecule-1 were associated with DFU healing. Furthermore, single-cell RNA...
Myocardial fibrosis is a hallmark of cardiac remodeling and functionally involved in heart failure development, leading cause deaths worldwide. Clinically, no therapeutic strategy available that specifically attenuates maladaptive responses fibroblasts, the effector cells heart. Therefore, our aim was to develop novel antifibrotic therapeutics based on naturally derived substance library screens for treatment fibrosis.Antifibrotic drug candidates were identified by functional screening 480...
Transforming growth factor (TGF)-β was linked to abnormal vessel function and can mediate impairment of endothelial angiogenic responses. Its effect on microRNAs downstream targets in this context is not known.To study the role TGF-β-mediated activity.MicroRNA profiling after TGF-β treatment cells identified miR-30a-3p, along with other members miR-30 family, be strongly silenced. Supplementation miR-30a-3p restored TGF-β-treated cells. We epigenetic methyl-CpG-binding protein 2 (MeCP2) a...
Diabetes mellitus predisposes to thrombotic and proliferative vascular remodeling, which thrombin contributes via activation of protease-activated receptor (PAR) 1. However, the use PAR-1 inhibitors suppress remodeling may be limited by severe bleeding. We recently reported upregulation an additional receptor, PAR-4, in human smooth muscle cells exposed high glucose have now examined PAR-4 as a novel mediator linking hyperglycemia, hypercoagulation, diabetes mellitus.PAR-4 expression was...
Biliary complications after liver transplantation remain a major cause of morbidity and reduced graft survival. Ischemic-type biliary lesions (ITBLs) are common difficult to treat. The pathophysiology ITBLs remains unclear, diagnostic markers still missing. analysis microRNA (miRNA) profiles is an evolving field in hepatology. Our aim was identify specific miRNA patterns the bile patients with transplantation. Liver transplant were included cross-sectional study. Patients (n = 37),...
We investigated the antihypertensive effect of a methanol extract (ME) fenugreek seeds and its fraction (MF) in deoxycorticosterone acetate (DOCA)-salt-induced fructose-induced hypertensive rats. In DOCA model, (15 mg/kg, twice week; s.c.) was administered uni-nephrectomized animals for 4 weeks. Methanol (30 mg kg−1 day−1; p.o.) were evaluated their effect. fructose drinking water replaced with 10% solution 6 weeks to induce hypertension. ME (100 assessed model. After completion treatment...
Diabetes is associated with vascular remodeling and increased thrombin generation. Thrombin promotes smooth muscle cell (SMC) mitogenesis migration via protease-activated receptors (PAR)-1, PAR-3, PAR-4. We investigated the effect of high glucose on expression function receptors.In human SMCs, (25 versus 5.5 mmol/L) induced a rapid sustained increase in PAR-4 mRNA, protein, surface expression. PAR-1 PAR-3 were not changed. High pretreatment (48 hours) enhanced or PAR-4-activating peptide but...
Since ancient times aging has also been regarded as a disease, and humankind always strived to extend the natural lifespan. Analyzing genes involved in disease allows for finding important indicators biological markers pathologies possible therapeutic targets. An example of use omics technologies is research regarding rare fatal premature syndrome progeria (Hutchinson-Gilford syndrome, HGPS). In our study, we focused on silico analysis differentially expressed (DEGs) aging, using publicly...