The aim of this study was to investigate the influence hi-maize, inulin, and rice bran in survival Lactobacillus acidophilus LA-5 pectin microparticles obtained by internal gelation subjected freeze-drying. For this, development a matrix capable extending viability develop new functional foods emphasized. Microparticle size, encapsulation efficiency, probiotic survivability after gastrointestinal simulation, storage stability were analyzed. + inulin presented highest efficiency (68.1%)...

Microcapsules containing B. lactis were produced by complex coacervation in gelatin and gum Arabic followed freeze drying characterized optical microscopy scanning electron microscopy, as well the resistance of probiotics to in-vitro release simulated gastrointestinal tract storage under different temperature conditions. Therefore, it formed microcapsules with high encapsulation efficiency (86.04% 99.52%) size between 100.12 203.32 μm. In addition, microencapsulated probiotics, both wet dry...

Emulsification/internal gelation is an encapsulation technique with great potential for protection of probiotics use in food products. In this study, microparticles containing Bifidobacterium BB-12 produced by emulsification using sodium alginate as wall material a slow release calcium ions were evaluated. Probiotics survival was studied resistance to simulated gastrointestinal conditions and stability during 120 days storage. The characterization the buffer solutions at different pH (4.5,...

The aim of this study was to develop and evaluate the physicochemical antioxidant stability nanoemulsions containing a Physalis peruviana calyx extract (CPp-NE) free extracts under different storage conditions (7 25 °C) with absence or incidence light for 120 days. were prepared ethanol 60% characterized later preparation by spontaneous emulsification. formulations presented nanometric sizes, low polydispersity index, negative zeta potential, acid pH, rutin content (11 μg·mL−1),...

ABSTRACT: Lactobacillus acidophillus La-5 (ML) and Bifidobacterium Bb-12 (MB) microparticles were produced at different temperatures by spray dryer. The influence of on the viability, encapsulation efficiency, water activity moisture evaluated. Microparticles that presented more viability submitted to thermal resistance, gastrointestinal simulation, storage stability, morphology particle size analyses. Drying temperature 130°C showed higher 84.61 79.73% for microparticles, respectively. In...

ABSTRACT: This study produced pectin microcapsules containing Lactobacillus acidophilus by external ionic gelation, followed the adsorption of whey protein and to form multilayers. The viability free microencapsulated lactobacilli was evaluated after in vitro exposure gastrointestinal conditions. They were also assessed heat treatment, stability examined at -18 °C, 5 °C 25 for 120 days. Exposure different pHs, simulating passage through tract, showed that treatment with only (LA/P0) one two...

Development and validation of LC-UV method to quantify daptomycin in injectable form, accordance with the ICH requirements.

ABSTRACT: The high intensity ultrasound-assisted extraction (HIU) is one of the most simple, quick and efficient techniques for phenolic other antioxidant compounds from plants. This first application HIU these goldenberry fruit. conventional showed similar results regarding to capacity. However, time required (5min) was 24 times lower than (120min). Phenolic reported were chlorogenic acid, caffeic acid rutin. In vitro cytotoxicity assays used evaluation extracts that in a wide range...

Abstract A set of chemometric tools including in silico simulation and design experiments (DOE) was used to develop validate an RP-LC/UV method for darifenacin hydrobromide. Retention factor (k′) from resolution (R) between degradation product were assessed by exploratory study using organic modifier type, content, mobile phase pH, flow rate, column oven temperature as factors a full factorial 25 design. The significant (organic temperature) selected applied central composite (CCD) with...

An accurate, simple, reproducible, and sensitive liquid chromatographic method was developed validated for the determination of omeprazole in powder injection pellets. The analyses were performed at room temperature on a reversed-phase C18 column 250 x 4.6 mm id, 5 microm particle size. mobile phase, composed methanol-water (90 + 10, v/v), pumped constant flow rate 1.5 mL/min. Detection UV detector 301 nm. terms linearity, precision, accuracy, ruggedness. response linear range 32-48...

A stability-indicating liquid chromatographic method has been developed for the quantitative determination of lodenafil carbonate in tablets. The employs a Synergi Fusion C18 column (250 × 4.6 mm, i.d., 4 µm particle size), with mobile phase consisting mixture methanol-acetic acid 0.1 % pH 4.0 (65:35, v/v) and UV detection at 290 nm, using photodiode array detector. linear response (r = 0.9999) was observed range 10-80 µg/mL. showed good recoveries (average 100.3%) also intra inter-day...

The development of functional foods by adding bioactive faces many hurdles in the production new foods. Microencapsulation is a useful tool to facilitate protection these compounds foods, particular, probiotics, vitamins, minerals, fatty acids, etc. Various microencapsulation methods have been used order improve use ingredients promoting successful delivery substances gastrointestinal tract. Among techniques compounds, extrusion has employed obtain microparticles. In this review we will...

Abstract Granulocyte colony‐stimulating factor (G‐CSF) is a hematopoietic cytokine produced by recombinant DNA technology and used clinically to treat neutropenia. An isocratic high performance liquid chromatography procedure was developed for the assay of filgrastim in pharmaceutical formulations. HPLC separation carried out size‐exclusion on TSK gel G2000 SW column (60 cm × 7.5 mm I.D.). The mobile phase composed phosphoric acid (pH 2.5; 0.1 M), run at flow rate 1.0 mL min−1 with UV...

The aim of this study was to develop and validate a LC stability-indicating method for the quantitation desonide hair lotion currently marketed in Brazil, as well delineate upon photostability same pharmaceutical product. used RP-18 column (150 x 4.6 mm, 5 mm), with methanol-acetonitrile-triethylamine solution mobile phase (0.3 %, pH 4.0) (50:10:40, v/v/v) UV detection at 244 nm. satisfied all validation requirements, evidenced by good linearity (r > 0.9999, range from 100 μg mL-1), accuracy...

This work aimed to develop and validate a simple, fast low cost analytical method for the quantification of bioactive piperine in nanoemulsions by high performance liquid chromatography with UV detection. Nanoemulsions were prepared spontaneous emulsification their physicochemical properties evaluated. Considering chromatographic conditions, mobile phase was composed methanol:water (70:30, v/v), Gemini® C18 column, detection at 343 nm. The linear concentration range 5-50 μg mL-1 (r =...

ABSTRACT: Oil-in-water (O/W) nanoemulsion containing goldenberry extract was elaborated using a high-energy ultrasonic bath method. Physicochemical characterization of the formulation carried out by determining pH, mean droplet diameter, polydispersity index (PDI) and zeta potential. Nanoemulsion toxicity assessed in vitro assays with tumor non-tumor cell lines, vivo Caenorhabditis elegans. The pH 3.84, diameter 268 ± 7 nm, PDI 0.113 potential -13.94 mV. Results cytotoxicity employing cells...

<p>Three methods are proposed for the quantitative determination of raloxifene hydrochloride in pharmaceutical dosage form: ultraviolet method (UV) high performance liquid chromatography (HPLC) and micellar capillary electrophoresis (MEKC). These were developed validated showed good linearity, precision accuracy. Also they demonstrated to be specific robust. The HPLC MEKC tested regards stability indicating have this attribute. UV used methanol as solvent optimal wavelength at 284 nm,...

Many natural compounds, extracted from fruits and vegetables, have promising potential for maintaining promoting health as well preventing treating some diseases. In the food industry, use of plant extracts, an alternative to chemical or synthetic antioxidants antimicrobials combat foodborne pathogens, inhibiting lipid oxidation thus extending shelf life is increasing trend. However, most bioactive compounds present in these extracts such polyphenols, carotenoids, alkaloids, among others,...

This work describes the development and validation of an LC stability-indicating method for quantitation desonide lotion, followed by a photostability study same pharmaceutical form under exposure to UVA light (352 nm). The used RP-18 column, mobile phase composed methanol:acetonitrile:water pH 5.0 mixture (50:10:40) detection at 244 nm. ICH guidelines, its robustness was evaluated factorial design (24). All requirements were met, evidenced good linearity (r > 0.9999, in range from 10 100...

Microencapsulation is the protection of a compound and modulation its release now, several techniques are available among them there complex coacervation. The coacervation technique involves association two polymers presents some advantages over other such as possibility working with biopolymers, absence organic solvent temperature conditions in mild processing. Functional foods containing probiotic bacteria becoming increasingly popular market due to beneficial health effects attributed...