A5060106760- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Lymphatic System and Diseases
- Atherosclerosis and Cardiovascular Diseases
- Nuclear Receptors and Signaling
- Sympathectomy and Hyperhidrosis Treatments
- Mast cells and histamine
- Vitamin C and Antioxidants Research
- Apelin-related biomedical research
- TGF-β signaling in diseases
- Immune Cell Function and Interaction
- Angiogenesis and VEGF in Cancer
- NF-κB Signaling Pathways
- Vitamin D Research Studies
- Blood Coagulation and Thrombosis Mechanisms
- Urticaria and Related Conditions
Universität Ulm
2009-2019
University Medical Center
2018-2019
University of Tübingen
2009
University of Bern
2009
University of Concepción
2003
Human cells acquire vitamin C using two different transporter systems, the sodium-ascorbic acid co-transporters with specificity for ascorbic acid, and facilitative glucose transporters dehydroascorbic acid. There is no information on mechanism of transport across intestinal barrier, a step that determines bioavailability in humans. We used colon carcinoma cell line CaCo-2 as an<i>in vitro</i> model enterocyte-like cells. The results kinetics, sodium dependence, inhibition studies, reverse...
Previous work has shown that c-Myc is required for adequate vasculogenesis and angiogenesis. To further investigate the contribution of Myc to these processes, we conditionally expressed in embryonic endothelial cells using a tetracycline-regulated system. Endothelial overexpression resulted severe defects vascular Myc-expressing embryos undergo widespread edema formation multiple hemorrhagic lesions. They die between days 14.5 17.5. The changes permeability are not caused by deficiencies...
Abstract Objective Determine the effect of bradykinin on solute permeability and cellular junctional proteins in human dermis microvascular endothelial cells. Methods Cells were characterized by immunofluorescence fluorescence‐activated cell sorting. Macromolecular transport dextran albumin was monitored. Junctional protein expression phosphorylation determined immunoblot analyses. Intracellular calcium cAMP levels evaluated. Target gene at mRNA determined. Results Human cells comprised 97%...
Angioedema is a known adverse effect of renin-angiotensin-aldosterone system blockers. It characterized by non-inflammatory swelling the head and neck region, increased plasma tissue levels bradykinin (BK). In-depth elucidation bradykinin-dependent mechanisms microvascular destabilization are still needed to comprehend molecular pathways causing angioedema for rational design anti-edematous therapy. Therefore, BK-induced changes in endothelial permeability cellular architecture were...
Some forms of angioedema (AE) – like hereditary and ACE inhibitor induced AE are mainly mediated by increased levels the tissue hormone bradykinin (BK). We elucidated molecular pathways activated prior BK treatment in lymphatic endothelial cells (EC) to analyze their possible participation development BK-mediated AE.