A5074485063- Aquaculture disease management and microbiota
- Environmental Toxicology and Ecotoxicology
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Synthesis and biological activity
- Pharmaceutical and Antibiotic Environmental Impacts
- Drug Transport and Resistance Mechanisms
- Vitamin C and Antioxidants Research
- Aquaculture Nutrition and Growth
- Synthesis and Reactions of Organic Compounds
- Microbial infections and disease research
- Animal Virus Infections Studies
- Genetic and phenotypic traits in livestock
- Vitamin K Research Studies
- Crystal structures of chemical compounds
- Parasite Biology and Host Interactions
- Vitamin D Research Studies
- Metabolism, Diabetes, and Cancer
- Antioxidant Activity and Oxidative Stress
- Adipose Tissue and Metabolism
- Invertebrate Immune Response Mechanisms
- Synthesis of heterocyclic compounds
- Pancreatic function and diabetes
- Myxozoan Parasites in Aquatic Species
- Animal Nutrition and Physiology
Austral University of Chile
2016-2025
Pfizer (United States)
2024-2025
Interdisciplinary Center for Aquaculture Research
2016-2025
Pearl River Community College
2024
Millennium Nucleus of Ion Channel Associated Diseases
2015
Instituto de Química y Fisicoquímica Biológicas
2011
Memorial Sloan Kettering Cancer Center
1993-2004
University of Concepción
2002-2003
Howard Hughes Medical Institute
1993
Cellular glutathione levels may exceed vitamin C by 10-fold, generating the question about real antioxidant role that low intracellular concentrations of can play in presence a vast molar excess glutathione. We characterized metabolism and its relationship with primary cultures human endothelial cells oxidatively challenged treatment hydrogen peroxide or activated undergoing respiratory burst, analyzed manner which interacts to increase capacity cells. Our data indicate that: (i) express...
Genistein is a dietary-derived plant product that inhibits the activity of protein-tyrosine kinases. We show here it potent inhibitor mammalian facilitative hexose transporter GLUT1. In human HL-60 cells, which express GLUT1, genistein inhibited transport dehydroascorbic acid, deoxyglucose, and methylglucose in dose-dependent manner. Transport was not affected by daidzein, an inactive analog does inhibit kinase activity, or general protein staurosporine. uptake deoxyglucose acid Chinese...
DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 97:197-205 (2012) - DOI: https://doi.org/10.3354/dao02403 Broth medium for successful culture fish pathogen Piscirickettsia salmonis A. J. Yañez1,*, K. Valenzuela1, H. Silva1, Retamales2, Romero3, R. Enriquez3, Figueroa1, Claude1, Gonzalez4, Avendaño-Herrera2, G. Carcamo1 1Laboratorio de Enzimología, Instituto...
Piscirickettsia salmonis is a fastidious intracellular pathogen responsible for high mortality rates in farmed salmonids, with serious economic consequences the Chilean aquaculture industry. Oxytetracycline and florfenicol are most frequently used antibiotics against P. salmonis, but routine use could contribute to drug resistance. This study identified differentiated susceptibilities two strains, LF-89 AUSTRAL-005. The less susceptible isolate, AUSTRAL-005, also showed ethidium bromide...
Human cells acquire vitamin C using two different transporter systems, the sodium-ascorbic acid co-transporters with specificity for ascorbic acid, and facilitative glucose transporters dehydroascorbic acid. There is no information on mechanism of transport across intestinal barrier, a step that determines bioavailability in humans. We used colon carcinoma cell line CaCo-2 as an<i>in vitro</i> model enterocyte-like cells. The results kinetics, sodium dependence, inhibition studies, reverse...
We characterized the human Na+-ascorbic acid transporter SVCT2 and developed a basic model for transport cycle that challenges current view it functions as Na+-dependent transporter. The properties of are modulated by Ca2+/Mg2+ reciprocal functional interaction between Na+ ascorbic defines substrate binding order stoichiometry. increased rate in cooperative manner, decreasing Km without affecting Vmax, thus converting low affinity form into high Inversely, affected bimodal...
Piscirickettsia salmonis is the etiological agent of salmonid rickettsial septicemia, a disease that seriously affects industry. Despite efforts to genomically characterize P. salmonis, functional information on life cycle, pathogenesis mechanisms, diagnosis, treatment, and control this fish pathogen remain lacking. To address knowledge gap, present study conducted an in silico pan-genome analysis 19 strains from distinct geographic locations genogroups. Results revealed expected open 3,463...
Abstract The rapid growth of the salmon farming industry in Chile has led to appearance various viral, bacterial, parasitic, and fungal pathogens affecting farmed fish. Chilean suffered several health crises associated with high fish mortalities, such as caused by infectious anaemia virus (2007) harmful algal blooms (2016). In addition these events, marine farms are continuously affected outbreaks pathogens, including bacteria Piscirickettsia salmonis and, most recently, a reappearance...
Adhesion of E. coli to the urinary tract epithelium is a critical step in establishing infections. FimH an adhesin positioned on fimbrial tip which binds mannosylated proteins via its lectin domain (FimH LD ). interest as target vaccines prevent infections (UTI). Previously, difficulties obtaining purified recombinant from along with poor inherent immunogenicity have hindered development effective vaccine candidates. To overcome these challenges, we devised novel production method using...
The synthesis of (E)-1-(1-benzyl-5-methyl-1H-1,2,3-triazol-4-yl)-3-phenyl-2-propen-1-one derivatives was carried out in two steps, using benzylic chloride as starting material. structural determination intermediates and final products performed by spectroscopic methods: infrared spectroscopy, nuclear magnetic resonance spectroscopy mass spectrometry (IR, NMR, MS). In vitro evaluation cytotoxic activity on adherent non-adherent cells showed that triazole chalcones exhibited significant...
Piscine orthoreovirus (PRV) is a pathogen that causes heart and skeletal muscle inflammation in Salmo salar has also been linked to circulatory disorders other farmed salmonids, such as Oncorhynchus kisutch mykiss. The virus segmented, double-stranded RNA genome, which makes it possible undergo genetic reassortment increase its genomic diversity through point mutations. In this study, PRV was assessed using the full genome sequences available public databases. This study used were...
The development of oil and gas production together with the fruit in nearby areas North Patagonia, Argentina, suggests aquatic pollution scenarios which include permanent combined short events pesticides application. It has been reported that hydrocarbons activate aryl hydrocarbon receptor (AhR) pathway rainbow trout, Oncorhynchus mykiss, insecticide Chlorpyrifos (CPF) interacts these effects. Thus, it is interesting to investigate whether insecticides, applied by separate or combined, can...
We report here the draft genome sequence of a lethal pathogen farmed salmonids, Piscirickettsia salmonis strain AUSTRAL-005. This virulent was isolated in 2008 from Oncorhynchus mykiss farms, and multiple genes involved pathogenicity, environmental adaptation, metabolic pathways were identified.
Gossypol is a natural disesquiterpene that blocks the activity of mammalian facilitative hexose transporter GLUT1. In human HL-60 cells, which express GLUT1, Chinese hamster ovary cells overexpressing and erythrocytes, gossypol inhibited transport in concentration-dependent fashion, indicating blocking GLUT1 independent cellular context. With exception red blood inhibition was instantaneous. effect specific for since it did not alter uptake nicotinamide by erythrocytes. affects...