A5062647135- Prion Diseases and Protein Misfolding
- Vitamin C and Antioxidants Research
- Vitamin K Research Studies
- Vitamin D Research Studies
- Nicotinic Acetylcholine Receptors Study
- Alzheimer's disease research and treatments
- Ion channel regulation and function
- Neurological diseases and metabolism
- Neuroscience and Neuropharmacology Research
- Trace Elements in Health
- Hepatitis B Virus Studies
- Phytochemistry and biological activities of Ficus species
- Garlic and Onion Studies
- Bioactive Compounds and Antitumor Agents
- Climate Change and Health Impacts
- Fungal Biology and Applications
- Synthesis and biological activity
- Brucella: diagnosis, epidemiology, treatment
- Ziziphus Jujuba Studies and Applications
- Liver physiology and pathology
- Blood properties and coagulation
- Chemical synthesis and alkaloids
- Receptor Mechanisms and Signaling
- Liver Disease and Transplantation
- Tryptophan and brain disorders
University of Concepción
2004-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2017-2020
Virologie et Immunologie Moléculaires
2013-2020
Université Paris-Saclay
2017
Cellular glutathione levels may exceed vitamin C by 10-fold, generating the question about real antioxidant role that low intracellular concentrations of can play in presence a vast molar excess glutathione. We characterized metabolism and its relationship with primary cultures human endothelial cells oxidatively challenged treatment hydrogen peroxide or activated undergoing respiratory burst, analyzed manner which interacts to increase capacity cells. Our data indicate that: (i) express...
Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by cognitive impairment that increasingly affects the elderly. AD's main features have been related to cellular and molecular events, including aberrant aggregation of amyloid beta peptide (Aβ), Ca2+ dyshomeostasis, increased mitochondria-associated membranes (MAMs). Transglutaminase type 2 (TG2) ubiquitous enzyme whose primary role Ca2+-dependent proteins transamidation, Aβ peptide. TG2 activity has closely damage death....
Ionotropic glycine receptors (GlyRs) are chloride-permeable ligand-gated ion channels expressed in the nervous system. Alterations to glycinergic inhibition and generation of dysfunctional GlyRs have been linked chronic pain, a widely prevalent disease. Positive allosteric modulators (PAMs) targeting exerted analgesic effects, motivating research on PAMs as potential pain therapies. Rationally designed tricyclic sulfonamides novel with activity. However, detailed electrophysiological studies...
In the development of vaccines capable providing immunity against brucellosis, Cu-Zn superoxide dismutase (SOD) has been demonstrated to be one protective immunogens Brucella abortus. an earlier study, we provided strong evidence that intramuscular injection with a plasmid DNA carrying SOD gene (pcDNA-SOD) was able induce immune response. The present study designed characterize T-cell responses after intraspleen (i.s.) vaccination BALB/c mice pcDNA-SOD. Animals vaccinated pcDNA-SOD did not...
Abstract Glycine receptors (GlyRs) are anion-permeable pentameric ligand-gated ion channels (pLGICs). The GlyR activation is critical for the control of key neurophysiological functions, such as motor coordination, respiratory control, muscle tone and pain processing. relevance function further highlighted by presence abnormal glycinergic inhibition in many pathophysiological states, hyperekplexia, epilepsy, autism chronic pain. In this context, previous studies have shown that functional...
The Gelsemium elegans plant preparations have shown beneficial activity against common diseases, including chronic pain and anxiety. Nevertheless, their clinical uses are limited by toxicity. Gelsemine, one of the most abundant alkaloids in plants, replicated these therapeutic toxic actions experimental behavioral models. However, molecular targets underlying biological effects remain unclear. profile gelsemine suggests involvement GABAA receptors (GABAARs), which main benzodiazepines...
Glycine receptors (GlyRs) are members of the pentameric ligand-gated ionic channel family (pLGICs) and mediate fast inhibitory neurotransmission in brain stem spinal cord. The function GlyRs can be modulated by positive allosteric modulators (PAMs). So far, it is largely accepted that both extracellular (ECD) transmembrane (TMD) domains constitute primary target for many these PAMs. On other hand, contribution intracellular domain (ICD) to PAM effects on remains poorly understood. To gain...
ABSTRACT Mammalian prions are PrP proteins with altered structures causing transmissible fatal neurodegenerative diseases. They self-perpetuating through formation of beta-sheet-rich assemblies that seed conformational change cellular PrP. Pathological usually forms an insoluble protease-resistant core exhibiting beta-sheet but no more alpha-helical content, loosing the three alpha-helices contained in correctly folded The lack a high-resolution prion structure makes it difficult to...
The data presented in this article are related to the research paper entitled "Increased expression of mitochondrial sodium-coupled ascorbic acid transporter-2 (mitSVCT2) as a central feature breast cancer", available Free Radical Biology and Medicine Journal [1]. In article, we examined SVCT2 transporter various cancer cell lines using RT-PCR Western blot assays. addition, analyzed subcellular localization by immunofluorescence colocalization assays cellular fractionation experiments....
Prions result from a drastic conformational change of the host-encoded cellular prion protein (PrP), leading to formation β-sheet–rich, insoluble, and protease-resistant self-replicating assemblies (PrPSc). The molecular mechanisms involved in spontaneous sporadic inherited human diseases or equivalent animal are poorly understood, part because cell models spontaneously forming prions currently lacking. Here, extending studies on role H2 α-helix C terminus PrP, we found that deletion highly...
Garlic has been used for decades as an important food and additionally its beneficial properties in terms of nutrition ancestral therapeutics. In this work, we compare the fresh (WG) aged (BG) extract obtained from elephant garlic, harvested on Chiloe Island, Chile. BG was prepared WG with a 20-day aging process under controlled temperature humidity conditions. We observed that BG, compounds such diallyl disulfide decrease, interest 5-hydroxymethylfurfural (69%), sulfide (17%),...
Mapping out regions of PrP influencing prion conversion remains a challenging issue complicated by the lack structure. The portion associated with infectivity contains α-helical domain correctly folded protein and turns into β-sheet-rich insoluble core in prions. Deletions performed so far inside this segment essentially prevented conversion. Recently we found that deletion last C-terminal residues helix H2 was fully compatible RK13-ovPrP cell culture model, using 3 different infecting...