A5063285966- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- Hypothalamic control of reproductive hormones
- Reproductive tract infections research
- Circular RNAs in diseases
- Neurogenesis and neuroplasticity mechanisms
- Genomics and Chromatin Dynamics
- Aquaculture Nutrition and Growth
- Reproductive System and Pregnancy
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- Cleft Lip and Palate Research
- Extracellular vesicles in disease
- Parasitic Infections and Diagnostics
- Congenital gastrointestinal and neural anomalies
- Prenatal Screening and Diagnostics
- Developmental Biology and Gene Regulation
- Hedgehog Signaling Pathway Studies
- Estrogen and related hormone effects
- Cancer-related gene regulation
- RNA modifications and cancer
- FOXO transcription factor regulation
- Cancer Cells and Metastasis
Instituto Tecnológico de Chascomús
2016-2025
Intech (Slovakia)
2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2024
National University of General San Martín
2005-2024
California Institute of Technology
2009-2010
Tokyo University of Marine Science and Technology
2007
The pejerrey <i>(Odontesthes bonariensis)</i> is a teleost fish with strong temperature-dependent sex determination (TSD). Several studies have shown that <i>dmrt1</i> and gonadal aromatase <i>(cyp19a1)</i> are implicated in the differentiation process teleosts but little known on expression balance endocrine regulation of these two genes during TSD. This study was designed to clarify patterns both reared at female-, male- mixed-sex-producing temperatures...
Here, we explore whether silencing via promoter DNA methylation plays a role in neural versus crest cell lineage decisions. We show that methyltransferase3A (DNMT3A) promotes specification by directly mediating repression of genes like Sox2 and Sox3. DNMT3A is expressed the plate border, its knockdown causes ectopic Sox3 expression at expense markers. In vivo chromatin immunoprecipitation folds demonstrates specifically associates with CpG islands regions, resulting their methylation. Thus,...
Abstract Folate is an essential vitamin for vertebrate embryo development. Methotrexate (MTX) a folate antagonist that widely prescribed autoimmune diseases, blood and solid organ malignancies, dermatologic diseases. Although it highly contraindicated pregnant women, because associated with increased risk of multiple birth defects, the effect paternal MTX exposure on their offspring has been largely unexplored. Here, we found treatment adult medaka male fish ( Oryzias latipes ) causes...
Neural crest cells form within the neural tube and then undergo an epithelial to mesenchymal transition (EMT) initiate migration distant locations. The transcriptional repressor Snail2 has been implicated in EMT via as of yet unknown mechanism. We report that adaptor protein PHD12 is highly expressed before EMT. At cranial levels, loss phenocopies knockdown, preventing shutdown adhesion molecule Cad6b (Cadherin6b), thereby inhibiting emigration. Although not directly binding each other, both...
Abstract Cytochrome P450 aromatase (cyp19) is an enzyme that catalyzes the conversion of androgens to estrogens and may play a role in temperature‐dependent sex determination (TSD) reptiles, amphibians, fishes. In this study, ovarian form ( cyp19A1 ) pejerrey Odontesthes bonariensis , teleost with marked TSD, was cloned its expression profile evaluated during gonadal differentiation at feminizing (17°C, 100% females), mixed‐sex producing (24 25°C, 73.3 26.7% females, respectively),...
Trichomoniasis, caused by the parasite Trichomonas vaginalis , is most common non-viral sexually transmitted infection. Current treatment relies exclusively on 5-nitroimidazole drugs, with metronidazole (MTZ) as primary option. However, increasing prevalence of MTZ-resistant strains poses a significant challenge, particularly in current absence alternative therapies. Several studies have revealed that development resistance T. linked to genomic and transcriptional alterations. Given role...
The central nervous system of adult teleost fish is peculiar because the following features: (1) persistence radial glial cells, (2) an important neurogenic activity and (3) a high aromatase expression by cells. In this study, proliferative zones forebrain were described using bromodeoxyuridine (BrdU) treatment in brain pejerrey, Acanthopterygian fish. These cells shown to have morphological immunoreactive characteristics express aromatase. Three different progenitor populations identified...
Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogenital tract. Infections range from asymptomatic to highly inflammatory, depending on host and strain. Different T. strains vary greatly in their adherence cytolytic capacities. These phenotypic differences might be attributed differentially expressed genes as consequence of extra-genetic variation, such epigenetic modifications. In this study, we explored role histone acetylation regulating gene...
Significance Mutations of DNA methyltransferase 3B (DNMT3B) result in facial abnormalities and neurologic defects humans related to abnormal neural crest development. To address the underlying mechanism, we tested role DNMT3B using loss- of-function molecular analyses chick embryos. Neural cells initially form within central nervous system (CNS), then migrate away elements skeleton peripheral (PNS). Our results show that acts by directly methylating promoter region gene Sox10 tube...
ABSTRACT miR-203 is a tumor-suppressor microRNA with known functions in cancer metastasis. Here, we explore its normal developmental role the context of neural crest development. During epithelial-to-mesenchymal transition cells to emigrate from tube, displays reciprocal expression pattern key regulators delamination, Phf12 and Snail2, interacts their 3′UTRs. We show that ectopic maintenance inhibits migration chick, whereas functional inhibition using ‘sponge’ vector or morpholinos promotes...
In vertebrates, the inner ear arises from otic placode, a thickened swathe of ectoderm that invaginates to form vesicle. We report histone demethylase KDM4B is dynamically expressed during early stages chick formation. A loss results in defective invagination and striking morphological changes epithelium, characterized by abnormal localization adhesion cytoskeletal molecules reduced expression several markers, including Dlx3. vivo chromatin immunoprecipitation reveals direct dynamic...
Trichomonas vaginalis is a common sexually transmitted parasite that colonizes the human urogenital tract causing infections range from asymptomatic to highly inflammatory. Recent works have highlighted importance of histone modifications in regulation transcription and pathogenesis. However, nature DNA methylation remains unexplored. Using combination immunological techniques ultrahigh-performance liquid chromatography (UHPLC), we analyzed abundance strains with differential pathogenicity...
The neural plate border (NPB) of vertebrate embryos segregates from the and epidermal regions, it is comprised an intermingled group multipotent progenitor cells. Folate precursor S-adenosylmethionine, main methyl donor for DNA methylation, critical embryonic development, including specification progenitors which reside in NPB. Despite fact that several intersecting signals involved territorial restriction NPB cells are known, role epigenetics, particularly has been a matter debate. Here, we...