A5012437630- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Glioma Diagnosis and Treatment
- Advanced Chemical Sensor Technologies
- Molecular Biology Techniques and Applications
- Advanced Proteomics Techniques and Applications
- PI3K/AKT/mTOR signaling in cancer
- Renal Transplantation Outcomes and Treatments
- Platelet Disorders and Treatments
- Hemostasis and retained surgical items
- Hemophilia Treatment and Research
- Boron Compounds in Chemistry
- Protein Kinase Regulation and GTPase Signaling
- Orthopedic Infections and Treatments
- Liver Disease and Transplantation
- Cancer, Hypoxia, and Metabolism
- Viral Infectious Diseases and Gene Expression in Insects
- Organ Transplantation Techniques and Outcomes
Nicolaus Copernicus University
2016-2022
Collegium Medicum in Bydgoszcz
2016-2022
Normothermic ex situ liver perfusion (NEsLP) offers the opportunity to assess biomarkers of graft function and injury. We investigated NEsLP parameters (biomarkers markers) for assessment viability in a porcine transplantation model. Grafts from heart-beating donors (HBD), with 30 minutes (donation after cardiac death [DCD]30'), 70 (DCD70'), 120 (DCD120') warm ischemia were studied. The HBD, DCD30', DCD70'-groups had 100% survival. In contrast, 70% developed primary nonfunction (PNF) died...
The development of a fast and accurate intraoperative method that enables the differentiation stratification cancerous lesions is still challenging problem in laboratory medicine. Therefore, it important to find optimize simple effective analytical enabling selection distinctive metabolites. This study aims assess usefulness solid-phase microextraction (SPME) probes as sampling for lipidomic analysis brain tumors. To this end, SPME was applied sample tumors immediately after excision,...
Alterations in the carnitine shuttle system may be an indication of presence cancer. As such, in-depth analyses this pathway different malignant tumors could important for detection and treatment disease. The current study aims to assess profiles acylcarnitines gliomas with respect their grade, isocitrate dehydrogenase (IDH) mutations, 1p/19q co-deletion. Brain obtained from 19 patients were sampled on-site using solid-phase microextraction (SPME) immediately following excision. Analytes...
Glioblastoma multiforme is one of the most malignant neoplasms among humans in their third and fourth decades life, which evidenced by short patient survival times rapid tumor-cell proliferation after radiation chemotherapy. At present, diagnosis gliomas decisions related to therapeutic strategies are based on genetic testing histological analysis tumor, with molecular biomarkers still being sought complement diagnostic panel. This work aims enable metabolomic characterization cancer tissue...
Despite the variety of tools available for cancer diagnosis and classification, methods that enable fast simple characterization tumors are still in need. In recent years, mass spectrometry has become a method choice untargeted profiling discriminatory compound as potential biomarkers disease. Biofluids generally considered preferable matrices given their accessibility easier sample processing while direct tissue provides more selective information about cancer. Preparation tissues analysis...
The development of a fast and accurate intraoperative method that enables the differentiation stratification cancerous lesions is still challenging problem in laboratory medicine. Therefore, it important to find optimize simple effective analytical selection distinctive metabolites. This study aims assess usefulness solid-phase microextraction (SPME) probes as sampling for lipidomic analysis brain tumors. To this end, SPME was applied sample tumors immediately after excision, followed by via...
Introduction: Nowadays, diagnosis of brain tumours is mainly carried out via neuroimaging techniques. The most widespread methods for routine analysis include computer tomography and magnetic resonance imaging. While such are useful to localise tumours, they unable offer a conclusive the tumour type. A final can only be made histological examination tissue after resection, or, in cases where location not amenable biopsy out. Untargeted metabolite relatively new approach diagnostics, capable...
Despite the variety of tools available for cancer diagnosis and classification, methods that enable fast simple characterization tumors are still in need. In recent years, mass spectrometry has become a method choice untargeted profiling discriminatory compound as potential biomarkers disease. Biofluids generally considered preferable matrices given their accessibility easier sample processing while direct tissue provides more selective information about cancer. Preparation tissues analysis...
Abstract BACKGROUND Histopathology is a gold standard for diagnostics of brain tumors but it time consuming, demands removal the part tissue what makes highly invasive. That why seems to be relevant develop new diagnostic methods addressing abovementioned drawbacks. Ideal tool should easy use, as low invasive possible and high clinical value. The aim study was assess usefulness chemical biopsy based on solid phase microextraction sampling method lipidomic analysis tumors. MATERIAL AND...